IUE(胚胎电转).ppt

,*,单击此处编辑母版文本样式,第二级,单击此处编辑母版标题样式,IUE,intro,Figure 1 Anatomical organization of the developing forebrain.(A)Schema of a sagittal section through the brain of an E12.5 mouse showing the main subdivisions of the forebrain,the diencephalon and the telencephalon.In the telencephalon,the pallium is depicted in lighter gray than the subpallium.,(B)Schema of a transversal section through the telencephalon of an E12.5 mouse,indicating some of its main subdivisions.LGE,lateral ganglionic eminence;,MGE,medial ganglionic eminence;POA,anterior preoptic area.,-Oscar Marin,2003 Neurosci,-,Susan K.McConnell,1995 Neuron,-Amitabh Gupta,2002 Nature,-,-Emilie Pacary,2012,Journal of Visualized Experiments,Surgical,-,Renping Zhou,2013,Neural Development:Methods and Protocols,Methods in Molecular Biology,Preparation of the skin(ad)Four continuous steps to prepare the skin for further embryo manipulation,Exposure of the embryos(ad)Four continuous steps to expose the embryos,Injection of DNA and electroporation(ac)Three continuous steps to inject DNA to embryonic brain ventricles followed by electroporation(d),Suturing of muscle and skin(a,b)Two steps to close the abdominal wall,thank you!,