淡水、海水培养基配方.doc

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SE Medium Working solution (g/l) NaNO3 0.25g K2HPO4 . 3H2O 0.075g MgSO4 . 7H2O 0.075g CaCl2 . 2H2O 0.025g KH2PO4 0.175g NaCl 0.025 Soil extract * 40ml FeCl3·6H2O 0.005 Fe—EDTA 1ml A5 solution * 1 distilled water 958 Soil Extract(土壤提取液)配置方法取花园土未施过肥0。5kg置于烧杯或三角瓶中，加入蒸馏水1000毫升，瓶口用透气塞封口，在水浴中沸水加热2小时，冷却数小时，在无菌条件下过滤，取上清液，将灭菌蒸馏水加入上清液至总体积1000毫升。土壤提取液保存在4ºC备用。 * Composition of the A5 solution Add to 100 ml of distilled water: H3BO3 286 mg MnCl2 . 4H2O 181 mg ZnSO4 . 7H2O 22 mg CuSO4 . 5 H2O 7.9 mg (NH4)6Mo7O24 . 4H2O 3.9 mg EDTA-Fe的配置方法：将EDTA和FeCl3·6H2O分别溶于水和HCl(0.1N),混匀即可。 Na2EDTA 1g distilled water 50ml FeCl3·6H2O 81 mg HCl（0.1N） 50ml f/2 Medium (Guillard & Ryther 1962, Guillard 1975) To 950 mL filtered seawater add: Quantity Compound Stock Solution Molar Concentration in Final Medium 1 mL NaNO3 75 g/L dH2O 8.83 x 10-4 M 1 mL NaH2PO4 · H2O 5 g/L dH2O 3.63 x 10-5 M 1 mL * Na2SiO3 · 9H2O* 30 g/L dH2O* 1.07 x 10-4 M* 1 mL f/2 trace metal solution (see recipe below) - 0.5 mL f/2 vitamin solution (see recipe below) - Make final volume up to 1 L with filtered seawater and autoclave. *Note: Autoclaved f/2 medium produces extensive silica precipitate. We delete silicate when it is not required by the alga (see f/2-Si medium below). f/2 Trace Metal Solution (Guillard & Ryther 1962, Guillard 1975) To 950 mL dH2O add: Quantity Compound Stock Solution Molar Concentration in Final Medium 3.15 g FeCl3 · 6H2O - 1 x 10-5 M 4.36 g Na2EDTA · 2H2O - 1 x 10-5 M 1 mL CuSO4 · 5H2O 9.8 g/L dH2O 4 x 10-8 M 1 mL Na2MoO4 · 2H2O 6.3 g/L dH2O 3 x 10-8 M 1 mL ZnSO4 · 7H2O 22.0 g/L dH2O 8 x 10-8 M 1 mL CoCl2 · 6H2O 10.0 g/L dH2O 5 x 10-8 M 1 mL MnCl2 · 4H2O 180.0 g/L dH2O 9 x 10-7 M Make final volume up to 1 L with dH2O. Autoclave.

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