常见酶切位点与保护碱基.doc

不同内切酶对识别位点以外最少保护碱基数目的要求
不同内切酶对识别位点以外最少保护碱基数目的要求
酶
寡核苷酸序列
切割率%
2 hr
20 hr
Acc I
GGTCGACC
CGGTCGACCG
CCGGTCGACCGG
0
0
0
0
0
0
Afl III
CACATGTG
CCACATGTGG
CCCACATGTGGG
0
>90
>90
0
>90
>90
Asc I
GGCGCGCC
AGGCGCGCCT
TTGGCGCGCCAA
>90
>90
>90
>90
>90
>90
Ava I
CCCCGGGG
CCCCCGGGGG
TCCCCCGGGGGA
50
>90
>90
>90
>90
>90
BamH I
CGGATCCG
CGGGATCCCG
CGCGGATCCGCG
10
>90
>90
25
>90
>90
Bgl II
CAGATCTG
GAAGATCTTC
GGAAGATCTTCC
0
75
25
0
>90
>90
BssH II
GGCGCGCC
AGGCGCGCCT
TTGGCGCGCCAA
0
0
50
0
0
>90
BstE II
GGGT(A/T)ACCC
0
10
BstX I
AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA
CTGCAGAACCAATGCATTGGATGCAT
0
25
25
0
50
>90
Cla I
CATCGATG
GATCGATC
CCATCGATGG
CCCATCGATGGG
0
0
>90
50
0
0
>90
50
EcoR I
GGAATTCC
CGGAATTCCG
CCGGAATTCCGG
>90
>90
>90
>90
>90
>90
Hae III
GGGGCCCC
AGCGGCCGCT
TTGCGGCCGCAA
>90
>90
>90
>90
>90
>90
Hind III
CAAGCTTG
CCAAGCTTGG
CCCAAGCTTGGG
0
0
10
0
0
75
Kpn I
GGGTACCC
GGGGTACCCC
CGGGGTACCCCG
0
>90
>90
0
>90
>90
Mlu I
GACGCGTC
CGACGCGTCG
0
25
0
50
Nco I
CCCATGGG
CATGCCATGGCATG
0
50
0
75
Nde I
CCATATGG
CCCATATGGG
CGCCATATGGCG
GGGTTTCATATGAAACCC
GGAATTCCATATGGAATTCC
GGGAATTCCATATGGAATTCCC
0
0
0
0
75
75
0
0
0
0
>90
>90
Nhe I
GGCTAGCC
CGGCTAGCCG
CTAGCTAGCTAG
0
10
10
0
25
50

酶
寡核苷酸序列
切割率%
2 hr
20 hr
Not I
TTGCGGCCGCAA
ATTTGCGGCCGCTTTA
AAATATGCGGCCGCTATAAA
ATAAGAATGCGGCCGCTAAACTAT
AAGGAAAAAAGCGGCCGCAAAAGGAAAA
0
10
10
25
25
0
10
10
90
>90
Nsi I
TGCATGCATGCA
CCAATGCATTGGTTCTGCAGTT
10
>90
>90
>90
Pac I
TTAATTAA
GTTAATTAAC
CCTTAATTAAGG
0
0
0
0
25
>90
Pme I
GTTTAAAC
GGTTTAAACC
GGGTTTAAACCC
AGCTTTGTTTAAACGGCGCGCCGG
0
0
0
75
0
25
50
>90
Pst I
GCTGCAGC
TGCACTGCAGTGCA
AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA
CTGCAGAACCAATGCATTGGATGCAT
0
10
>90
>90
0
0
10
>90
>90
0
Pvu I
CCGATCGG
ATCGATCGAT
TCGCGATCGCGA
0
10
0
0
25
10
Sac I
CGAGCTCG
10
10
Sac II
GCCGCGGC
TCCCCGCGGGGA
0
50
0
>90
Sal I
GTCGACGTCAAAAGGCCATAGCGGCCGC
GCGTCGACGTCTTGGCCATAGCGGCCGCGG
ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA
0
10
10
0
50
75
Sca I
GAGTACTC
AAAAGTACTTTT
10
75
25
75
Sma I
CCCGGG
CCCCGGGG
CCCCCGGGGG
TCCCCCGGGGGA
0
0
10
>90
10
10
50
>90
Spe I
GACTAGTC
GGACTAGTCC
CGGACTAGTCCG
CTAGACTAGTCTAG
10
10
0
0
>90
>90
50
50
Sph I
GGCATGCC
CATGCATGCATG
ACATGCATGCATGT
0
0
10
0
25
50
Stu I
AAGGCCTT
GAAGGCCTTC
AAAAGGCCTTTT
>90
>90
>90
>90
>90
>90
Xba I
CTCTAGAG
GCTCTAGAGC
TGCTCTAGAGCA
CTAGTCTAGACTAG
0
>90
75
75
0
>90
>90
>90
Xho I
CCTCGAGG
CCCTCGAGGG
CCGCTCGAGCGG
0
10
10
0
25
75
Xma I
CCCCGGGG
CCCCCGGGGG
CCCCCCGGGGGG
TCCCCCCGGGGGGA
0
25
50
>90
0
75
>90
>90