1、不同内切酶对识别位点以外最少保护碱基数目的要求 不同内切酶对识别位点以外最少保护碱基数目的要求 酶 寡核苷酸序列 切割率% 2 hr 20 hr Acc I GGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 0 0 0 0 Afl III CACATGTG CCACATGTGG CCCACATGTGGG 0 >90 >90 0 >90 >90 Asc I GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA >90 >90 >90
2、 >90 >90 >90 Ava I CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 50 >90 >90 >90 >90 >90 BamH I CGGATCCG CGGGATCCCG CGCGGATCCGCG 10 >90 >90 25 >90 >90 Bgl II CAGATCTG GAAGATCTTC GGAAGATCTTCC 0 75 25 0 >90 >90 BssH II GGCGCGCC AGGCGCGCCT TTGG
3、CGCGCCAA 0 0 50 0 0 >90 BstE II GGGT(A/T)ACCC 0 10 BstX I AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT 0 25 25 0 50 >90 Cla I CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG 0 0 >90 50 0 0 >90 50 EcoR I GGA
4、ATTCC CGGAATTCCG CCGGAATTCCGG >90 >90 >90 >90 >90 >90 Hae III GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA >90 >90 >90 >90 >90 >90 Hind III CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG 0 0 10 0 0 75 Kpn I GGGTACCC GGGGTACCCC CGGGGTACCCCG 0 >90 >90 0
5、>90 >90 Mlu I GACGCGTC CGACGCGTCG 0 25 0 50 Nco I CCCATGGG CATGCCATGGCATG 0 50 0 75 Nde I CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC 0 0 0 0 75 75 0 0 0 0 >90 >90 Nhe I GGCTAG
6、CC CGGCTAGCCG CTAGCTAGCTAG 0 10 10 0 25 50 酶 寡核苷酸序列 切割率% 2 hr 20 hr Not I TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA 0 10 10 25 25 0 10 10 90 >90 Nsi I TGCATGCATGCA CCAATGCATTGGTTCT
7、GCAGTT 10 >90 >90 >90 Pac I TTAATTAA GTTAATTAAC CCTTAATTAAGG 0 0 0 0 25 >90 Pme I GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG 0 0 0 75 0 25 50 >90 Pst I GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGA
8、ACCAATGCATTGGATGCAT 0 10 >90 >90 0 0 10 >90 >90 0 Pvu I CCGATCGG ATCGATCGAT TCGCGATCGCGA 0 10 0 0 25 10 Sac I CGAGCTCG 10 10 Sac II GCCGCGGC TCCCCGCGGGGA 0 50 0 >90 Sal I GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGG
9、CCATAGCGGCCGCGGAA 0 10 10 0 50 75 Sca I GAGTACTC AAAAGTACTTTT 10 75 25 75 Sma I CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 0 0 10 >90 10 10 50 >90 Spe I GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG 10 10 0 0 >90 >90 50 50 Sph I GGCATG
10、CC CATGCATGCATG ACATGCATGCATGT 0 0 10 0 25 50 Stu I AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT >90 >90 >90 >90 >90 >90 Xba I CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG 0 >90 75 75 0 >90 >90 >90 Xho I CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG 0 10 10 0 25 75 Xma I CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA 0 25 50 >90 0 75 >90 >90






