简述影响羊水细胞培养成功率的因素及羊水中细胞在组织工程领域的研究概况.doc

方法 根据 IL-10 mRNA基因序列，设计共合成引物，利用体外转录系统，制作地高辛标记的RNA探针，建立检测IL-10原位杂交方法
结果 在总共23批的IL-10原位杂交实验中，其中有8次实验出现阳性率较低的阳性结果
结论 在鼻咽组织中检测IL-10的表达是可行的，但在福尔马林固定的组织中，mRNA大部分被破坏，所以在实验中有些组织呈阴性，有些组织呈弱阳性，在临床病理中探究IL-10表达与鼻咽癌的发病机制作用关系仍有很大的研究价值
Methods
Result in situ hybridization experiments, including experiments show positive rates of 8 positive results with lower

Conclusion In the nose pharynx organization expression of detecting IL - 10 is feasible, but in formalin fixed organization, mRNA most damage, so in experiments some organizations negative, some organizations are weak positive in clinical pathology, explore IL - 10 in the pathogenesis of nasopharyngeal carcinoma and express the effect relation still has great value for study

中文摘要
目的 鼻咽组织中IL-10与抑制性T细胞的关系实验体系的一部分，如能成功探索出石蜡切片上的原位杂交方法来检测IL-10阳性，将有助于深化对鼻咽癌发病机制的认识。
方法 根据 IL-10 mRNA基因序列，设计共合成引物，利用体外转录系统，制作地高辛标记的RNA探针，建立检测IL-10原位杂交方法
结果 在总共23批的IL-10原位杂交实验中，其中有8次实验出现阳性率较低的阳性结果
结论 在鼻咽组织中检测IL-10的表达是可行的，但在福尔马林固定的组织中，mRNA大部分被破坏，所以在实验中有些组织呈阴性，有些组织呈弱阳性，在临床病理中探究IL-10表达与鼻咽癌的发病机制作用关系仍有很大的研究价值
关键词：基因表达；原位杂交；IL-10细胞因子

Abstract
Objective the relationship between IL-10 of Nasopharyngeal tissues and inhibited T cell is part of the experimental system, if a method in situ hybridization can be explored successfully to detect IL-10, it will help to deepen the understanding of pathogenesis of nasopharyngeal carcinoma
Methods According to IL - 10 mRNA gene sequences, co-synthetic primer can be designed and through transcription systems in vitro , we can make the RNA probe with digoxin and the method in situ hybridization for detecting IL-10 can be also established .
Results During the 23 batches in total of IL-10 in situ hybridization experiments, 8 appears positive,but the positive was low.
Conclusion Detection the expression of IL-10 gene in the nasopharyngeal tissue is feasible, but in the fixed tissues fixed with formalin, mRNA was largely destroyed, so some organizations in the experiment were negative, and some were weakly positive, but it’s still worth researshing to study the relations between the expression of IL-10 and the pathogenesis of NPC in clinical pathology.