限制性内切酶保护碱基.doc

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限制性内切酶保护碱基A-B 时间:2010-4-18 11:12:04 酶切效率: - 0% - 0-20% - 20-50% - 50-100% * - 反应时间为16小时酶保护碱基数目 1 2 3 4 5 AarI 20-50 50-100 AasI 50-100 AatII 0 0-20 20-50 50-100 Acc65I 0-20 50-100 AdeI 50-100 AluI 0 20-50 50-100 Alw21I 50-100 Alw26I 50-100 Alw44I 0 20-50 50-100 ApaI 50-100 BamHI 50-100 BcnI 20-50 50-100 BclI 0 50-100 BcuI 50-100 BfiI 50-100 BfmI 50-100 BfuI 50-100 BglI 20-50 50-100 BglII 0 50-100 Bme1390I 20-50 50-100 BoxI 0 50-100 BpiI 50-100 Bpu10I 20-50 50-100 Bpu1102I 50-100 BseDI 0 50-100 BseGI 50-100 BseJI 0 50-100 BseLI 0 50-100 BseMI 0-20 50-100 BseMII 50-100 BseNI 0 50-100 BseSI 50-100 BseXI 20-50 50-100 Bsh1236I 50-100 Bsh1285I 0-20 50-100 BshNI 50-100 BshTI 20-50 50-100 Bsp68I 0 50-100 Bsp119I 50-100 Bsp120I 20-50 50-100 Bsp143I 50-100 Bsp143II 0 50-100 Bsp1407I 20-50 50-100 BspLI 50-100 BspPI 0 50-100 BspTI 0 0-20 50-100 Bst1107I 0-20 50-100 BstXI 0 50-100 Bsu15I 50-100 BsuRI 0-20 20-50 50-100 限制性内切酶保护碱基C-E 时间:2010-4-18 11:14:12 酶切效率: - 0% - 0-20% - 20-50% - 50-100% * - 反应时间为16小时酶保护碱基数目 1 2 3 4 5 CaiI 0 0-20 50-100 CfrI 0 50-100 Cfr9I 20-50 50-100 Cfr10I 20-50 50-100 Cfr13I 50-100 Cfr42I 50-100 CpoI 50-100 Csp6I 50-100 DpnI 50-100 DraI 0 0-20 50-100 Eam1104I 0 50-100 Eam1105I 0 50-100 Ecl136II 50-100 Eco24I 50-100 Eco31I 20-50 50-100 Eco32I 20-50 50-100 Eco47I 50-100 Eco47III 0 0-20 50-100 Eco52I 0-20 50-100 Eco57I 50-100 Eco57MI 50-100 Eco72I 0-20 50-100 Eco81I 50-100 Eco88I 50-100 Eco91I 20-50 50-100 Eco105I 20-50 50-100 Eco130I 0 50-100 Eco147I 0 50-100 EcoO109I 50-100 EcoRI 50-100 EherI 20-50 50-100 Esp3rI 50-100 限制性内切酶保护碱基F-N 时间:2010-4-18 11:15:55 酶切效率: - 0% - 0-20% - 20-50% - 50-100% * - 反应时间为16小时酶保护碱基数目 1 2 3 4 5 FaqI 0-20 50-100 FspAI 0-20 20-50 50-100 FspBI 20-50 50-100 GsuI 50-100 Hin1I 50-100 Hin1II 0 0-20 50-100 Hin6I 0 0-20 50-100 HincII 50-100 HindIII 0 0-20 50-100 HinfI 50-100 HpaII 0-20 50-100 HphI 0 50-100 Hpy8I 0-20 50-100 HpyF10VI 50-100 KpnI 50-100 Kpn2I 0 50-100 KspAI 20-50 50-100 LweI 20-50 50-100 MbiI 0 0-20 50-100 MboI 50-100 MboII 50-100 MlsI 0-20 50-100 MluI 20-50 50-100 MnlI 0 50-100 Mph1103I 20-50 50-100 MspI 0-20 50-100 MssI 20-50 50-100 MunI 20-50 50-100 MvaI 0 20-50 50-100 Mva1269I 0 50-100 NcoI 0 50-100 NdeI* 0-20 20-50 50-100 NheI 0 20-50 50-100 NmuCI 20-50 50-100 NotI 20-50 50-100 NsbI 0 0-20 50-100 限制性内切酶保护碱基O-X 时间:2010-4-18 11:17:30 酶切效率: - 0% - 0-20% - 20-50% - 50-100% * - 反应时间为16小时酶保护碱基数目 1 2 3 4 5 OliI 0-20 20-50 50-100 PaeI 0 0-20 20-50 50-100 PagI 20-50 50-100 PauI 0 50-100 PdiI 0-20 50-100 PdmI 50-100 PfeI 50-100 Pfl23II 0-20 20-50 50-100 PfoI 0 20-50 50-100 Psp5II 0 50-100 Psp1406I 0-20 50-100 PsuI 0-20 50-100 PstI 0-20 50-100 PsyI 0 50-100 PvuI 20-50 50-100 PvuII 50-100 RsaI 50-100 SacI 50-100 SalI 20-50 50-100 SatI 0 50-100 ScaI 0-20 50-100 SchI 50-100 SdaI 0-20 50-100 SduI 50-100 SfiI 50-100 SmaI 50-100 SmiI 0-20 50-100 SmuI 50-100 SsiI 50-100 SspI 0-20 50-100 TaaI 20-50 50-100 TaiI 50-100 TaqI 0 20-50 50-100 TasI 0 20-50 50-100 TatI 0-20 20-50 50-100 TauI 20-50 50-100 Tru1I 0 0-20 50-100 Van91I 0 50-100 VspI 50-100 XagI 20-50 50-100 XapI 20-50 50-100 XbaI 20-50 50-100 XceI 0 50-100 XhoI 0-20 50-100 XmaJI 50-100 XmiI 50-100 I-SceI 50-100

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