ATCC菌株活化说明--2019英文版.pdf

TIB.081 Revision J Page 1 of 22 Recommended Culture Methods for Microorganisms Selection of Growth Requirements 1.Primary growth on a nonselective agar medium is preferred.Primary growth in a fluid medium should only occur in special instances or when recommended.Because of the manipulations required during hydration,it is difficult to obtain purity of a lyophilized strain in a fluid medium.A contaminant may completely overgrow and obscure the presence of the lyophilized strain.2.The following information lists which method should be used to grow the various microorganism species.Descriptions of methods follow the microorganism list.Microorganism Method Notes Acetobacter species Method 36 Achromobacter species Method 1 Acinetobacter species Method 1 Actinobacillus species Method 3 Actinomyces species Method 4 Aerococcus species Method 1 Aeromonas species Method 2 Exceptions are Aeromonas hydrophila,Microbiologics 0870 and Aeromonas salmoncida Aeromonas hydrophila,Microbiologics 0870 Method 31 Aeromonas salmonicida Method 32 Aggregatibacter species Method 3 Alcaligenes species Method 1 Alicyclobacillus species Method 12 An exception is Alicyclobacillus acidoterrestris.Alicyclobacillus acidoterrestris Method 45 Alloiococcus species Method 2 Alternaria species Method 5 Amylomyces species Method 5 Aneurinibacillus species Method 1 Aquaspirillum species Method 20.Arcanobacterium species Method 34 Arthrobacter species Method 21 Aspergillus species Method 5 An exception is Aspergillus flavus.Aspergillus flavus Method 46 Aureobasidum species Method 5 Bacillus species Method 49 Bacteroides species Method 4 An exception is Bacteroides ureolyticus.Bacteroides ureolyticus Method 38 Bifidobacterium species Method 4 An exception is Bifidobacterium animalis subsp.animalis.TIB.081 Revision J Page 2 of 22 Microorganism Method Notes Bifidobacterium animalis subsp.animalis.Method 39 Bordetella bronchiseptica Method 15 Bordetella parapertussis Method 16 Bordetella pertussis Method 16 Exceptions are Bordetella pertussis,Microbiologics catalog numbers 0100 and 0843.Bordetella pertussis Microbiologics 0100 and 0843 Method 57 Brevibacillus species Method 1 Brevundimonas species Method 1 Brochothrix species Method 21 Budvicia species Method 21 Burkholderia species Method 1 Campylobacter species Method 6 Candida species Method 5 Capnocytophaga species Method 3 Cedecea species Method 1 Cellulosimicrobium species Method 1 Chaetomium species Method 5 Chryseobacterium species Method 1 An exception is Chryseobacterium shigense.Chryseobacterium shigense Method 22 Citrobacter species Method 1 Cladosporium species Method 5 Clostridium species Method 40 Exceptions are Clostridium difficile,Clostridium perfringens,Clostridium sordellii,and Clostridium tetani.Clostridium difficile Method 4 Clostridium perfringens Method 41 An exception is Clostridium perfringens,Microbiologics 0318.Clostridium perfringens Microbiologics 0318 Method 63 Clostridium sordellii Method 4 Clostridium tetani Method 4 Corynebacterium species Method 1 An exception is Corynebacterium urealyticum.Corynebacterium urealyticum Method 2 Cronobacter species Method 1 Cryptococcus species Method 62 An exception is Cryptococcus gattii.Cryptococcus gattii Method 47 Curtobacterium species Method 1 Deinococcus species Method 1 An exception is Deinococcus radiophilus,Microbiologics 01184.Deinococcus radiophilus Microbiologics 01184 Method 23 Delftia species Method 1 TIB.081 Revision J Page 3 of 22 Microorganism Method Notes Desulfotomaculum species Method 17 Edwardsiella species Method 1 Eggerthella species Method 4 Eikenella species Method 3 Elizabethkingia species Method 1 Enterobacter species Method 1 Enterococcus species Method 1 Erysipelothrix species Method 2 Escherichia coli Method 1 Eurotium rubrum Method 5 Exiguobacterium species Method 1 Finegoldia species Method 42 Fluoribacter species Method 8 Fusarium species Method 5 Fusobacterium species Method 4 An exception is Fusobacterium mortiferum,Microbiologics 01191.Fusobacterium mortiferum Microbiologics 01191 Method 39 Gardnerella species Method 9 Gemella species Method 4 Geobacillus species Method 24 An exception is Geobacillus stearothermophilus,Microbiologics 0137.Geobacillus stearothermophilus Method 64 Geotrichum species Method 5 Granulicatella adiacens Method 19 Haemophilus species Method 3 Hafnia species Method 1 Hanseniaspora species Method 5 Herminiimonas species Method 20 Issatchenkia species Method 5 Kingella species Method 33 Klebsiella species Method 1 Kloeckera species Method 5 Kocuria species Method 1 An exception is Kocuria rosea.Kocuria rosea Method 21 Lactobacillus species Method 65 Exceptions are Lactobacillus acidophilus,Lactobacillus casei,Lactobacillus gasseri,and Lactobacillus leichmanni.Lactobacillus acidophilus Method 11 Lactobacillus casei Method 11 Lactobacillus gasseri Method 11 Lactobacillus leichmannii Method 11 TIB.081 Revision J Page 4 of 22 Microorganism Method Notes Lactococcus species Method 2 Leclercia species Method 1 Legionella species Method 8 Listeria species Method 1 Lysinibacillus species Method 1 Macrococcus species Method 1 Magnusiomyces capitatus Method 5 Malassezia species Method 14 Mannheimia species Method 1 Methylobacterium species Method 25 An exception is Methylobacterium extorquens.Methylobacterium extorquens Method 26 Meyerozyma guilliermondii Method 5 Microbacterium species Method 22 Micrococcus species Method 1 An exception is Micrococcus luteus,Microbiologics 0337 and Micrococcus luteus,Microbiologics 0689.Micrococcus luteus,Microbiologics 0337 Method 27 Micrococcus luteus,Microbiologics 0689 Method 67 Microsporum species Method 5 Exceptions are Microsporum canis and Microsporum gypseum.Microsporum canis Method 48 Microsporum gypseum Method 73 Moraxella species Method 2 Morganella species Method 1 Mucor racemosus Method 5 Mycobacterium species Method 13 Exceptions are Mycobacterium fortuitum,Mycobacterium peregrinum and Mycobacterium haemophilum,and Mycobacterium smegmatis.Mycobacterium fortuitum Method 7 Mycobacterium haemophilum Method 18 Mycobacterium peregrinum Method 7 Mycobacterium smegmatis Method 7 Mycoplasma bovis Method 60 Mycoplasma hominis Method 58 Mycoplasma pneumoniae Method 59 Myroides species Method 2 Neisseria species Method 37 Nocardia species Method 81 Novosphingobium species Method 21 Ochrobactrum species Method 1 Oligella species Method 2 TIB.081 Revision J Page 5 of 22 Microorganism Method Notes Paecilomyces species Method 5 Paenibacillus species Method 1 An exception is Paenibacillus larvae.Paenibacillus larvae Method 22 Parabacteroides species Method 4 Parvimonas species Method 43 Pasteurella species Method 2 Pediococcus species Method 11 An exception is Pediococcus damnosus.Pediococcus damnosus Method 55 Penicillium species Method 5 Peptoniphilus species Method 42 Peptostreptococcus species Method 4 Plesiomonas species Method 1 Pluralibacter gergoviae Method 1 Porphyromonas species Method 43 Prevotella species Method 43 Propionibacterium species Method 44 Proteus species Method 1 An exception is Proteus hauseri.Proteus hauseri Method 27 Prototheca species Method 5 Providencia species Method 1 Pseudomonas species Method 1 Exceptions are Pseudomonas aeruginosa,Microbiologics 0484,Pseudomonas benneri,Pseudomonas fluorescens,Pseudomonas fragi,Pseudomonas protegens,Pseudomonas mosselii,Pseudomonas putida,and Pseudomonas species,Microbiologics 0162.Pseudomonas aeruginosa,Microbiologics 0484 Method 28 Pseudomonas brenneri Method 21 Pseudomonas fluorescens Method 21 Pseudomonas fragi Method 21 Pseudomonas mosselii Method 21 Pseudomonas protegens Method 21 Pseudomonas putida,Microbiologics 0627 and 0702 Method 22 Pseudomonas species,Microbiologics 0162 Method 22 Ralstonia species Method 1 An exception is Ralstonia pickettii,Microbiologics 01197.Ralstonia pickettii,Microbiologics 01197 Method 29 Raoultella species Method 1 Rhizopus species Method 5 Rhizobium radiobacter Method 21 TIB.081 Revision J Page 6 of 22 Microorganism Method Notes Rhodococcus species Method 2 Rhodotorula species Method 5 Saccharomyces species Method 50 Salmonella species Method 1 Scopulariopsis species Method 5 Serratia species Method 1 Shewanella species Method 1 Exceptions are Shewanella haliotis and Shewanella putrefaciens.Shewanella haliotis Method 74 Shewanella putrefaciens Method 75 Shigella species Method 1 Sphingobacterium species Method 1 Sphingomonas species Method 21 Sporidobolus species Method 5 Staphylococcus species Method 1 An exception is Staphylococcus aureus,Microbiologics 0158.Staphylococcus aureus,Microbiologics 0158 Method 30 Stenotrophomonas species Method 22 Streptococcus species Method 34 Exceptions are Streptococcus criceti,Streptococcus species,Microbiologics 0978,and Streptococcus pneumoniae.Streptococcus criceti Method 35 Streptococcus pneumoniae Method 66 Streptococcus species Microbiologics 0978 Method 66 Streptomyces species Method 5 Talaromyces pinophilus Method 5 Thermoanaerobacterium species Method 56 Trichoderma species Method 5 Trichophyton species Method 51 Trichosporon species Method 5 Trueperella pyogenes Method 34 Ureaplasma species Method 61 Veillonella species Method 4 Vibrio species Method 10 An exception is Vibrio alginolyticus.Vibrio alginolyticus,Microbiologics 0819 Method 54 Virgibacillus species Method 1 Wallemia mellicola Method 5 Yarrowia species Method 5 Yersinia species Method 1 An exception is Yersinia ruckeri,Microbiologics 0785.TIB.081 Revision J Page 7 of 22 Microorganism Method Notes Yersinia ruckeri,Microbiologics 0785 Method 21 Zygosaccharomyces species Method 5 Exceptions are Zygosaccharomyces rouxii,Microbiologics 0803;Zygosaccharomyces bisporus,Microbiologics 0960;and Zygosaccharomyces parabailii,Microbiologics 01011.Zygosaccharomyces bisporus,Microbiologics 0960 Method 53 Zygosaccharomyces rouxii,Microbiologics 0803 Method 53 Zygosaccharomyces parabailii,Microbiologics 01011 Method 52 3.The following information lists methods for growing microorganisms.When possible,more than one type of agar medium per method is listed.Method 1 Media Tryptic Soy Agar(Soybean Casein Digest Agar),Nonselective Sheep Blood Agar,Standard Methods Agar(Plate Count Agar)or Nutrient Agar Temperature 35C Atmosphere Aerobic Growth Time 24 to 48 hours Method 2 Media Nonselective Sheep Blood Agar Temperature 35C Atmosphere Aerobic Growth Time 24 to 72 hours Method 3 Media Chocolate Agar Temperature 35C Atmosphere 5 to 7%Carbon Dioxide Growth Time 24 to 48 hours Method 4 Media Anaerobic Blood Agar Temperature 35C Atmosphere Anaerobic Growth Time 48 to 72 hours Note:Some obligate anaerobes may require 5-7 days to demonstrate sufficient growth.TIB.081 Revision J Page 8 of 22 Method 5 Media Sabouraud Dextrose Emmons Agar Temperature 25C Atmosphere Aerobic Growth Time 2 to 7 days Note:Nonselective Sheep Blood Agar is an appropriate alternative.Nutrient Agar,Tryptic Soy Agar,Potato Dextrose Agar,and Standard Methods Agar(Plate Count Agar)are appropriate alternatives together with an additional period(24 hours)of incubation.Method 6 Media Chocolate Agar Temperature 35C Atmosphere Microaerophilic Growth Time 48 to 72 hours Note:Do not open the inoculated agar medium petri plate for the first 48 hours.Method 7 Media Lowenstein Jensen Agar or Middlebrook Agar Temperature 35C Atmosphere 5 to 7%CO2 or aerobic atmosphere.Growth Time 2 to 30 days Note:M.fortuitum subssp.fortuitum,M.smegmatis,M.peregrinum will also grow on Tryptic Soy Agar(Soybean Casein Digest Agar)as well as Lowenstein Jensen and Middlebrook Agar but additional incubation time may be required.Method 8 Media Buffered Charcoal Yeast Extract Agar Temperature 35C Atmosphere Aerobic Growth Time 3 to 5 days Method 9 Media V Agar or Chocolate Agar Temperature 35C Atmosphere 5 to 7%Carbon Dioxide Growth Time 48 hours Method 10 Media Tryptic Soy Agar(Soybean Casein Digest Agar)or Marine Agar Temperature 35C Atmosphere Aerobic Growth Time 24 to 48 hours Note:Rehydrate in sterile Brain Heart Infusion Broth,Tryptic Soy Broth,or 0.85%Saline.Transfer a portion of hydrated material immediately to agar.Incubate aerobically at 35C for 24 to 48 hours.Note:Rehydration with water may result in decreased or no recovery.Rehydration with fluid provided in the KWIK-STIK unit provides satisfactory recovery when grown on the recommended media.TIB.081 Revision J Page 9 of 22 Method 11 Media Phase 1:MRS(Man,Rogosa,Sharpe)Broth Phase 2:Columbia CNA with Sheep Blood or Tryptic Soy Agar with Sheep Blood Temperature Phase 1:35C Phase 2:35C Atmosphere Phase 1:Aerobic Phase 2:5 to 7%Carbon Dioxide Growth Time Phase 1:48 hours Phase 2:48 hours Note:For Phase 1,the primary growth medium is MRS(Man,Rogosa,Sharpe)Broth.Incubate at 35C in aerobic atmosphere for 48 hours.For Phase 2,transfer to either Columbia CNA with Sheep Blood or Tryptic Soy Agar with Sheep Blood using a sterile swab or pipette.Incubate at 35C in 5 to 7%carbon dioxide for 48 hours.Method 12 Media Potato Dextrose Agar Temperature 55C Atmosphere Aerobic Growth Time 24 to 48 hours Method 13 Media Lowenstein Jensen Agar or Middlebrook Agar Temperature 35C Atmosphere 5 to 7%CO2 or Aerobic Growth Time May require up to one month of incubation.Method 14 Media Leeming Notman Agar Temperature 30C Atmosphere Aerobic Growth Time 72 hours Method 15 Media Chocolate agar,Sheep Blood Agar,Tryptic Soy Agar,and Bordet Gengou Agar with 15%Defibrinated Sheep Blood Temperature 35C Atmosphere Aerobic Growth Time 24 to 48 hours Note:Standard Methods Agar(Plate Count Agar)or Nutrient Agar are appropriate alternatives together with an additional period(24 hours)of incubation.TIB.081 Revision J Page 10 of 22 Method 16 Media Chocolate agar or Bordet Gengou Agar with 15%Defibrinated Sheep Blood Temperature 35C Atmosphere Aerobic Growth Time 2 days to 1 week Method 17 Media Phase 1:ISF(modified Infant Soy Formula)Broth Phase 2:Sulfite Agar Temperature Phase 1:55C Phase 2:55C Atmosphere Phase 1:Anaerobic Phase 2:Anaerobic Growth Time Phase 1:48 hours Phase 2:48 hours to 7 days Prepare and use ISF(modified Infant Soy Formula)Broth using the following steps:1.Fill tubes with 10 ml Infant Soy Formula.Infant Soy Formula may be purchased at a grocery store.2.Place a four-penny nail in each tube.A four-penny nail is approximately 1.5 inches,or 38 mm,in length.It should contain steel or iron.3.Sterilize the broth.4.Inoculate ISF Broth with one LYFO DISK or KWIK-STIK.5.Grow at 55C in anaerobic conditions for 48 hours.The broth will turn grey,indicating growth.6.Make two dilutions,1:10 and 1:100.7.Subculture with a swab to Sulfite Agar.Plate the undiluted sample and the 1:10 and 1:100 dilutions.It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see.Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite.8.Incubate the agar in anaerobic environment at 55C for 48 hours to 7 days.Me