Agilent_硝基呋喃_LCMSMS_分析条件-孙春业.docx

Agilent 硝基呋喃LCMSMS分析方法
Agilent 1200 Series RRLC/uHPLC System
Flow Rate (ml/min)
: 0.4
Column
: Agilent Poroshell120 EC-C18 3.0 x100mm 2.7um
Column Oven (℃)
: 35
Injection Volume
: 10-20uL
Mobile Phase A
: 5mMol AA + 0.1%Fa in water
Mobile Phase B
: Acetonitrile
Run Time (min)
A (%)
B (%)
Flow Rate (ml/min)
0
80
20
0.4
1
80
20
0.4
9
60
40
0.4
10
5
95
0.4
12
5
95
0.4
12.1
80
20
0.4
Post Time : 3.0-5.0min
Time Segments
Index
Start Time
Scan Type
Div Valve
Delta EMV
Store
1
0
MRM
MS
200
Yes
2
4
MRM
MS
500
Yes
3
5.6
MRM
MS
100
Yes
Source Parameters (6410、6420、6430 & 6460-option100)
Source Type
: ESI
Dry Gas Temp (℃)
: 350
Polarity
: Positive
Capillary Voltage (V)
: 4000
Nebulizer Gas (psi)
: 45

Dry Gas (L/min)
: 11

Source Parameters (6470 & 6490)
Source Type
: AJS
Sheath Gas (L/min)
: 11
Polarity
: Positive
Sheath Gas Temp (℃)
: 350
Nebulizer (psi)
: 45
Capillary Voltage (V)
: 4000
Dry Gas (L/min)
: 10
Nozzle Voltage (V)
: 0
Dry Gas Temp (℃)
: 325

标准曲线

溶剂: 0.1% Fa水溶液
线性范围: 1-100 μg/L

MRM条件：
Time Segment 1
Compound
Name
Pre. Ion
MS1 Res
Pro. Ion
MS2 Res
Dwell
Frag (V)
CE (V)
Polarity
AMOZ
335.2
Unit
291.2
Unit
200
90
9
Pos
AMOZ
335.2
Unit
262.2
Unit
200
90
13
Pos

Time Segment 2
Compound
Name
Pre. Ion
MS1 Res
Pro. Ion
MS2 Res
Dwell
Frag (V)
CE (V)
Polarity
AHD
249.1
Wide
133.9
Wide
100
114
9
Pos
AHD
249.1
Wide
104
Wide
100
114
21
Pos
SEM
209
Wide
191.9
Wide
100
88
9
Pos
SEM
209
Wide
166
Wide
100
88
5
Pos

Time Segment 3
Compound
Name
Pre. Ion
MS1 Res
Pro. Ion
MS2 Res
Dwell
Frag (V)
CE (V)
Polarity
AOZ
236.1
Unit
134
Unit
200
110
9
Pos
AOZ
236.1
Unit
104
Unit
200
110
21
Pos

图谱结果：

注：以上图谱是1290-6460在10ppb浓度下，进样2uL所得到的结果