四川小麦品种HMW-GS组成及品质参数演变分析_蒋云.pdf

DOI：10.13430/ki.jpgr.20220908001植物遗传资源学报 2023，24（3）：744-757Journal of Plant Genetic Resources四川小麦品种HMW-GS组成及品质参数演变分析蒋云1，郝明2，刘登才2，吕季娟3，汤述尧1，宣朴4，郭元林1，陈谦1，王颖1，肖俊1，张洁1（1四川省农业科学院生物技术核技术研究所，成都 610061；2四川农业大学小麦研究所，温江 611130；3四川省种子站，成都 610041；4四川省农业科学院农产品加工研究所，成都 610061）摘要：分析了19492018年间，四川省的184个小麦育成品种、1个引进品种和2个地方品种的高分子量谷蛋白亚基（HMW-GS）组成和品质指标的演变。结果表明，Glu-1A位点有1、N、2*等3种亚基类型；Glu-1B位点有7、20、22、7+8、7+9、6+8、14+15和23+18等8种亚基类型；Glu-1D位点有5+10、2+12和3.1t+11*t等3种亚基类型。共检测到23种亚基组合类型，“N/7+9/5+10”组合出现频率最高，为12.30%。随着育种进程的发展，四川小麦育成品种的HMW-GS组成多样性有所增加，优质强筋亚基1和5+10的比率逐渐上升。四川小麦品种整体上属于中、弱筋类型，除容重、沉降值上升外，其余品质指标呈先上升后缓慢下降的趋势。本研究可为四川小麦品质的进一步改良提供参考。关键词：四川小麦；高分子量谷蛋白亚基；品质；演变分析Variations of HMW-GS and Quality-Related Parameters in Wheat Varieties Released in Sichuan ProvinceJIANG Yun1，HAO Ming2，LIU Deng-cai2，LYU Ji-juan3，TANG Shu-yao1，XUAN Pu4，GUO Yuan-lin1，CHEN Qian1，WANG Ying1，XIAO Jun1，ZHANG Jie1（1Biotechnology and Nuclear Technology Research Institute，Sichuan Academy of Agricultural Sciences，Chengdu 610061；2Triticeae Research Institute，Sichuan Agricultural University，Wenjiang 611130；3Seed Station of Sichuan Province，Chengdu 610041；4Institute of Agro-products Processing Science and Technology，Sichuan Academy of Agricultural Sciences，Chengdu 610061）Abstract：The high molecular weight glutenin subunits（HMW-GS）compositions and the quality parameters in 184 Sichuan wheat varieties（released from 1949 to 2018），one introduced variety and two landraces were analyzed.The results showed that there were three alleles at Glu-1A including 1，N，2*，eight at Glu-1B including 7，20，22，7+8，7+9，6+8，14+15 and 23+18，and three at Glu-1D including 5+10，2+12 and 3.1t+11*t.These subunits on sub-genomes A，B，D formed 23 allele combinations，and the combination（N/7+9/5+10）was found with a frequency of 12.3%.Taking advantage of breeding effects，the allele diversity at the Glu-1 locus，as well as the ratio of high-quality strong gluten 1（Glu-1A）and 5+10（Glu-1D）were gradually increased.收稿日期：2022-09-08 修回日期：2022-12-07 网络出版日期：2023-01-18URL：https：/doi.org/10.13430/ki.jpgr.20220908001第一作者研究方向为作物遗传育种，E-mail：通信作者：张洁，研究方向为作物遗传育种，E-mail：基金项目：国防科工局核能开发科研项目；四川省重点研发项目（2021YFYZ0002-05）；成都市科技项目（2021-YF05-02264-SN）；四川省财政专项（2021ZYGG-003）；四川省科技计划（2022ZDZX0014，2023NSFSC0220）；四川省农作物分子育种平台（No.2021YFYZ0027）；四川省农业科学院“1+9揭榜挂帅”项目（1+9 KJGG001，1+9 KJGG002）Foundation projects：Nuclear Energy Development and Research Project of State Administration of Science，Technology and Industry for National Defence；The Key Research and Development Program of Sichuan Province（2021YFYZ0002-05）；Chengdu Science and Technology Program（2021-YF05-02264-SN）；Special Project of Sichuan Provincial Finance Department（2021ZYGG-003）；Sichuan Science and Technology Program（2022ZDZX0014，2023NSFSC0220）；Crop Molecular Breeding Platform of Sichuan Province（No.2021YFYZ0027）；The 1+9 Open Competition Project of Sichuan Academy of Agricultural Sciences to Select the Best Candidates（1+9KJGG001，1+9 KJGG002）3 期蒋云等：四川小麦品种HMW-GS组成及品质参数演变分析The medium-weak gluten wheat varieties were found with predominant proportion in Sichuan province.The increase on test weight and sedimentation value，but the increase followed by decreased on other quality traits were observed.This study can provide reference for further improvement of wheat quality in Sichuan province，China.Key words：Sichuan wheat；high molecular weight gluten subunit；quality；evolution analysis随着品质研究的发展，“四川小麦品质差”等笼统的概念逐渐被专家学者摒弃，研究发现四川大部分小麦种植区适宜生产优质弱筋小麦，少数区域适宜生产中、强筋小麦1-3，但优质专用小麦品种少，大多品种达不到优质专用麦标准的重要因素是籽粒的贮藏蛋白含量和质量不平衡1，4-5。高分子量谷蛋白亚基（HMW-GS，high molecular weight glutenin subunits）是小麦籽粒中重要的贮藏蛋白，对小麦品质有重要影响，可解释小麦品种品质变异的 45%67%6-7。Payne8提出了 HMW-GS评分表，给不同的亚基赋予不同的分值，从而可以通过HMW-GS组成预测不同品种的面包烘烤品质。不仅如此，研究还表明HMW-GS组成对几乎所有小麦加工食品品质均有不同程度的影响，如Glu-1B位点的17+18亚基有利于提升面条品质，而7+8亚基则有利于提升馒头品质9-10。但是，四川省地方品种中缺乏优质HMW-GS，导致20世纪70年代以前四川小麦面筋质量普遍较差。颜济11指出，提高四川小麦面筋强度的重要措施是加强优质高分子量谷蛋白亚基5+10的育种应用，他率先将克里木卡小麦的5+10亚基成功转育到小麦品系70-5858中，并由此衍生出一系列含5+10亚基的小麦品系。近年来，通过大力引进利用优质亚基，四川小麦缺少优质亚基的状况得到了较大改善。Gao等12将河南、河北和四川小麦品种的HMW-GS组成结构进行了对比；杨恩年13对四川省“十五”期间育成的44个小麦新品种的HMW-GS组成进行了分析；周强等14、晏本菊等15、王相权等16分别对本单位育成的品种（系）的HMW-GS进行了研究，均发现四川省5+10亚基的频率比地方品种有了明显提高，四川省审定品种的HMW-GS品质评分高于全国平均水平和黄淮麦区水平。但这类研究着重对材料的HMW-GS组成进行描述，而对其品质效应报道较少。胡学旭等17认为，受生态条件限制，四川省内5+10优质亚基的品质效应弱，面筋质量依然低于全国和黄淮麦区。可见，高分子量谷蛋白亚基在四川生态条件下对小麦品质的效应较为特殊，值得进一步研究，对于指导未来四川小麦品质改良和推广应用具有积极意义。尽管关于四川小麦育成品种HMW-GS组成演变的报道不少，但前人所选材料大多是针对本单位育成的品种（系），缺乏代表性，而时间长度多以510年为单位进行研究。本研究以不同时期四川育成的187个品种（引进品种、地方品种）为材料，系统的分析了HMW-GS组成和品质性状的演变，可为育种者提供四川省小麦品种品质的基本信息，从而为小麦品质育种提供参考。1材料与方法1.1参试材料参试材料共187份，包括19492018年育成的184个小麦品种，占同时期四川省（不含凉山州和原重庆市）育成冬小麦品种的78.6%，1个引进品种和2个地方品种，育成品种由各育种单位惠赠，地方品种和引进品种由四川农业大学提供（表1）。其中，地方品种中国春（N/7+8/2+12）和现代品种川育12（1/7+8/5+10）、川育17（N/7+9/2+12）作为高分子量谷蛋白亚基标准对照，均由四川省农业科学院生物技术核技术研究所保存。1.2高分子量谷蛋白亚基提取及检测所有材料于2019-2020年度种植于四川省成都市郫都区试验基地，每份材料种植2行，行长1 m，行距27 cm，株距5 cm，田间管理与大田生产一致，HMW-GS提取及检测参考Yan等 18 的方法，采用十二烷基硫酸钠-聚丙烯酰氨凝胶电泳（SDS-PAGE）方法测定HMW-GS组成，其中分离胶浓度15%，浓缩胶浓度4.5%。HMW-GS的命名参照Payne等19的标准。电泳仪器为北京六一仪器厂生产的DYY-28B型电泳槽。1.3品质数据来源及分析品质数据来自历年四川省小麦区试总结，参数包括容重、蛋白质含量、湿面筋含量、沉降值和稳定时间等（表1）。小麦品质标准参照2018年四川省区试规定的小麦品质标准（表2）。数据处理及统计分析用Office excel 365和DPS数据处理软件（v7.05）完成20。745植物遗传资源学报24 卷表1参试材料及品质指标相关信息Table1Relevant information of test materials and quality indicators123456789101112131415161718192021222324252627282930313233343536373839404142434445464748495019491949194919491949194919491949196119611962196219641978198419841984198519881988198919891990199219931995199519951995199619971997199919991999199920002000200120012001200220022002200220022002200220022002阿勃五一麦山农205友谊麦大粒早成都光头开罗中国春繁6繁7大头黄竹叶青80-8川育5号川辐1号川育6号绵阳11宜宾1号川育9号绵阳20川辐3号川辐2号宜宾6号川育12川辐4号绵阳23绵阳24绵阳25绵阳26绵阳28川麦28绵阳27川育14宜麦7号川麦30绵阳29川农7号川麦107绵农6号川农11号川麦32川辐5号川育16川农16川麦36川育17川农12号川农17绵阳31川麦35引进品种成都光头/矮粒多/川福麦/碧玉麦中农28/合川光头阿夫/丰收麦S-6南大2419/康波多罗金堂县地方品种开县地方品种成都光头选系IBO1828/NP824/3/五一麦/成都光头/中农483/4/中农28B/IBO1828/NP824/阿夫繁6姊妹系成都光头分枝麦天然杂种雅安早姊妹系繁6变异株繁6姊妹系69-1776/大粒早同位素34P-射线处理川育5号繁6/川麦10号70-5858/繁6NP824/IBO1828/雅安早川育5号/3/NP824/夫诺/波兰小麦绵阳11号系选巴麦18/79P6007-射线辐照（川辐1号/78中2882）F171-5543/阿夫乐尔川育8号/83-4516-射线辐照（川辐1号/78中2882）F1绵阳19/79-2812绵阳01821/83选13028T7915K-3-69/绵阳15绵阳20/川育9号T7935/绵阳11号万雅2号/2874/高加索/2874/3/绵阳1981-5/川育9号9920/2164687016C-4-10/绵86-51426/4/IR68-77/YAA/ALSD/3/YAZ/ST2022/983绵阳11/95-3绵阳11/84-20142469/80-28-785-42/巴麦1878-5038/85-DH50751900“S”/Ning8349/1900-射线辐照（绵阳88-334/88-11525）F130020/8619-10/晋麦30川育12/87-429SW5193/Milan选系绵阳26/G295-491S-23/A30291S-23/A302绵阳90-310/川植89-076SW1862/2469790.0793.0759.0800.0657.0764.0798.0747.7780.0777.0784.0776.0753.0788.0764.0792.5770.8796.5802.0781.0774.0794.0793.9793.0752.0752.0776.0814.0743.0777.0819.0809.0785.0784.0794.012.7510.2713.8713.7813.4214.9612.0014.5015.3913.0216.8014.9612.9615.1314.5813.0315.0214.4714.5016.9014.3414.6315.8914.6011.3013.1013.3013.2012.5310.9013.6015.2014.0014.1011.5022.4827.1026.9828.0830.9030.4029.0028.4533.5034.5027.8032.8034.2027.9941.1030.7537.0047.3036.3035.1041.2933.8019.5028.5029.6028.8025.2020.3026.6040.3039.7035.1030.4060.0082.5029.7043.3018.2020.8030.0023.2516.2547.0025.0024.0019.7019.003.303.001.402.902.051.30编号No.审定年份Year of released品种名称Varieties name系谱Origin容重（g）Test weight粗蛋白含量（%）Crude protein content湿面筋含量（%）Wet gluten content沉降值（mL）Zeleny稳定时间（min）Stability time7463 期蒋云等：四川小麦品种HMW-GS组成及品质参数演变分析5152535455565758596061626364656667686970717273747576777879808182838485868788899091929394959697989910010110210320032003200320032003200320032003200320032003200320032004200420042004200420042004200420052005200520052005200520052005200520062006200620062006200620062007200720072007200720072007200720072008200820082008200820082008绵阳33川麦42川麦37川农10号内麦8号绵阳35川农19川育19蓉麦2号川麦38川育18绵阳30川麦41川麦44内麦9号内麦10号绵麦37良麦2号川农21绵麦38川麦43科成麦1号绵麦40绵麦39良麦3号川麦46川农23川农22川麦47川麦45川农26绵麦41绵麦43川育20川麦49川麦48绵麦42蜀麦375内麦11号绵麦1403西科麦4号川农25良麦4号川农24绵麦45川育21蜀麦482川麦50内麦836绵麦185绵麦46川麦51川麦521294/绵阳86-5Syn-CD768/SW89-3243/川641588繁8/88-30978-5038/85-DH5015绵阳26/92R17805363-8-1/绵优2号黔1104A/R935川育5号/墨460/绵阳2653283/90M776Syn-CD769/SW89-3243/川6415川育5号/墨460/94F2-4绵阳01821/83选13028/绵阳05520-1491T4135/88繁896夏440/贵农21绵阳26号/92R178绵阳26/92R17896EW37/绵阳90-100绵阳26/异源2号R841/黔恢3号07146-12-1/贵农19-4Syn-CD768/SW89-3243/川6415贵农22/川育12绵阳01821/贵农19-4绵阳96-78/贵农21-1N711/N301-1-1Ta193-6280/96-5429R1685/绵阳26R164/86-104Syn-CD786/绵阳26/绵阳26GH430/SW1862川农19/R3301绵阳01821/90中165/贵农19-407146-12-1/贵农19-4川麦30/35050/21530贵农21/生核3295川麦32/川麦86绵阳96-5/贵农21-1（绵阳93-7/92R141）F2/绵阳96-324品5/94-7绵阳04854/贵农21-1墨460/9601-396I-225/91S-5-4N1491/N1071白粒3号/云凡52894-207146-12-1/贵农19-4周88114/G159（绵阳93-7/92R141）F2/绵阳96-324贵农21/生核32955680/92R133绵阳96-5/辽春10号07242-3-1-1/贵农21174/183/99-1572川麦36/SW1862775.0778.0791.0758.0765.5795.0773.0803.0785.0779.0787.5760.0754.0761.3786.0776.0757.0772.0768.3774.0782.8776.0774.5779.0793.0787.0798.0815.0801.0781.0805.0799.0758.5798.0785.5776.0792.5813.0778.0787.0830.0803.0813.0819.0752.5829.0805.0810.0769.5734.0798.0796.0782.014.1014.1014.9010.5013.8014.0013.6015.6015.0012.8015.1011.6911.4414.2814.7315.5014.7614.6015.2115.7115.1713.5214.1014.4014.0014.4013.8016.3014.6014.8012.7012.5215.3314.5713.0713.9413.9612.1714.1613.4913.8613.1412.7413.3012.7912.9715.3613.5012.7713.0714.1113.1514.4735.4027.8539.6017.4033.3034.2535.5039.0041.8028.6038.1020.7522.7029.5028.5030.4029.6633.9030.6531.7032.8724.6027.5030.6028.8028.2029.5036.1032.7031.6025.4023.3528.1028.4026.6029.0031.2021.4027.9023.2025.8025.1022.8025.4022.2027.3030.8027.6024.6524.5029.1025.0029.4032.0036.7022.0019.8029.7028.7033.0027.4030.0025.6020.6014.0520.0038.9738.6037.9036.7216.9027.8831.5033.8842.6037.1036.3031.9029.0025.6034.5031.9027.8035.5023.9536.3024.1029.7030.1525.3531.2014.8025.0030.9023.3022.0021.7035.9022.4052.0030.3026.9038.9043.6042.5032.909.905.153.503.503.253.253.152.652.502.101.501.451.106.304.904.904.582.702.582.551.508.105.553.553.102.902.702.452.451.635.405.204.153.853.602.851.955.954.403.802.952.802.302.051.901.557.305.104.003.403.002.801.90表1（续）编号No.审定年份Year of released品种名称Varieties name系谱Origin容重（g）Test weight粗蛋白含量（%）Crude protein content湿面筋含量（%）Wet gluten content沉降值（mL）Zeleny稳定时间（min）Stability time747植物遗传资源学报24 卷10410510610710810911011111211311411511611711811912012112212312412512612712812913013113213313413513613713813914014114214314414514614714814915015115215315415515620092009200920092009200920092009201020102011201120112012201220122012201320132013201320132013201320132013201320142014201420142014201420142014201420142015201520152015201520152015201520152015201520152015201520152016川麦55川农27川麦53川麦54绵麦48科成麦2号川麦56川育24川麦58绵麦367国豪麦15川麦60绵麦228川麦104川麦61川麦62绵麦51内麦316川麦64蜀麦969川麦65川麦63蜀麦51西科8号南麦618特研麦南88绵麦1618资麦2号西科麦9号荣春南麦1号川麦90川麦80川麦91川麦67中科麦138中科麦47川麦66川育25川麦1131川麦68宜麦9号南麦991川麦1145川麦1247川农29西科麦10号川辐8号科成麦4号川麦81川辐7号川麦92绵麦285川育26SW3243/SW8688川农19/R3301477/绵农4号/Y31492R171/98间335/DH1523绵阳01821/贵农19-4咸阳大穗/E/多花-1/3/贵农20/4/绵阳26川麦30/川麦42周88114/G394川麦42/03间3/川麦42绵麦37/99-1522绵阳96-5/贵19-4/NE98-1231/贵农21/生核3295绵麦37/内2938/99-1522川麦42/川农16郑9023/间3/2/间3/3/1522Fr3/SW1862绵麦37/99-1522川农17号/品5川麦42/川农16SHW-L1/SW8188/川育18/3/川麦4298-1231/贵农21/生核329501-3570/川农23N711/N240197-392/云225747-52001-43-1/攀早抗16-1/攀早抗绵麦37/内2938/99-1522川农18变异株/川麦30内4301/绵阳31号16-1/攀早抗间38/99116/川麦42郑005/1522/1522内麦8号/郑9023/00062/3/川麦4299-1572/SW8688/01-3570川麦42/川育16W7268/硬粒小麦/川麦10799-1572/98-266/01-3570金顶-1/4105801-3570/川农2399-1572/98-266/01-3570川农12/宜97-2430-9-1/04-2-3301-3570/川农23L239-248-5/0722502017/R88/R13199-55/125739849-14-3/川农2437147/CD02-1574-3SW8019/99-1572/99-1572-射线辐照（02中5X/4093）F1内麦8号/间3/川麦42绵麦37/99-1522川育23/4/G219/3/川育16/H5/罗布林770.0802.5804.0740.0777.0791.5784.0816.0788.0750.5780.0789.0730.0795.0794.0795.0761.0794.5780.0778.0806.0813.0762.0749.5789.5797.0738.0827.0801.0837.0817.0839.0819.0834.0831.0851.0839.0757.0825.0777.7820.0784.0820.5756.5778.0756.0765.5803.0748.0778.0743.0727.5815.514.4815.5313.4915.3812.4514.1214.3114.1013.6912.5013.3714.1213.9014.5215.1614.2012.2114.3213.7614.5712.8714.9714.3015.3914.0214.9514.0814.9513.1113.6713.7013.2714.3013.1413.5513.7512.9714.0014.5011.6814.8413.3013.5011.1011.6012.8011.4213.2010.5011.7012.8011.1012.8929.2031.9026.2032.9024.2026.9029.0029.8027.8021.4025.3030.2030.3031.7034.5030.3724.0528.8026.6031.0027.9631.3029.8030.6028.1031.7027.6029.6025.3026.3026.2025.9026.9024.3026.1027.9023.7028.7028.2021.9530.3028.4027.0022.2022.4026.9021.4024.4021.2022.5024.7021.3026.1032.8012.1039.9036.6025.3027.5027.3030.3034.5029.2028.2032.5027.2032.5034.5024.0023.7548.5043.1047.3035.8043.8043.0041.8038.0046.8048.5053.8036.8055.3033.1027.8036.8030.9045.5032.0025.0029.7036.0031.0848.3047.5031.3027.3020.2031.0026.3027.5020.5022.2033.5028.5045.605.003.353.203.103.103.003.001.603.402.254.703.902.703.952.801.501.4010.008.808.106.805.605.105.004.303.301.9012.605.805.403.102.802.702.702.502.401.905.805.004.724.704.503.503.502.702.302.001.701.601.301.200.907.30表1（续）编号No.审定年份Year of released品种名称Varieties name系谱Origin容重（g）Test weight粗蛋白含量（%）Crude protein content湿面筋含量（%）Wet gluten content沉降值（mL）Zeleny稳定时间（min）Stability time7483 期蒋云等：四川小麦品种HMW-GS组成及品质参数演变分析1571581591601611621631641651661671681691701711721731741751761771781791801811821831841851861872016201620162016201620162016201620162017201720172017201720172017201720182018201820182018201820182018201820182018201820182018川农30西科麦18蜀麦126川麦601科成麦5号川麦1826蜀麦921内麦366绵麦112科成麦6号蜀麦133川麦602川麦82川农32蜀麦830川育27绵麦312川育29川麦1546绵麦1501川麦1557川麦604西科麦11川麦93嘉农麦809西科麦12川麦86川麦96南麦660蜀麦691川麦60303FR1349-1/54789/川农2799-55/1257川育23/B1291（贵农21/SW3243）F1/（川麦42/川麦44）F137147/川麦3701-3570/川农23030104-13/内麦11Balandal88/内4344绵06-367/99-1522CD-P35-1/98718/川育1230389/B1291（贵农21/SW3243）F1/川麦42Singh6/3*1231川农27/80978SHW-L1/川农16/Pm99915-1/3/03-DH1959川育23/SW8588/3/G349/30024/NE706贵农21/90M434/1227-185/99-1522川育23/中植3586/50669川麦104/川麦60贵农006/MY184805126/川05观8贵农21/SW3243/川麦42贵农06/J20普冰3504/川育20/川麦104川农23/川农1905R5404/内6072R4117/1572间3/川农19/川麦1043911/M49良麦2号/川育18矮败贵农21/绵阳26/川麦42811.5770.5815.5784.5801.5770.0789.5753.0691.0793.0778.0744.5770.0754.0759.0810.0767.0829.0784.5791.0802.0799.5804.0827.0791.5797.0822.0798.0758.5802.0792.013.5213.2913.2012.4913.9510.7813.3412.1010.5012.9912.5011.6011.4612.4512.6012.0611.7114.4012.9012.8012.7015.6012.3012.5013.5012.0012.3013.5011.9014.0013.6027.9024.9027.6025.3528.7522.1027.8022.8020.3025.9523.3022.4523.2025.3025.9022.7019.9528.3023.8025.8024.0032.9023.2024.9028.7021.9025.1026.4022.9028.9027.4039.7034.5041.9031.0031.1526.6039.7530.8025.3038.5029.7029.5030.6528.2520.8038.6028.00-5.003.002.802.502.202.201.701.701.205.703.303.053.002.502.001.651.156.604.504.303.803.003.002.802.702.702.401.901.901.601.50表1（续）编号No.审定年份Year of released品种名称Varieties name系谱Origin容重（g）Test weight粗蛋白含量（%）Crude protein content湿面筋含量（%）Wet gluten content沉降值（mL）Zeleny稳定时间（min）Stability time表示没有数据 indicates no data2结果与分析2.1四川小麦育成品种HMW-GS组合类型及频率对参试材料的HMW-GS组合类型及频率进行了SDS-PAGE分析（图1）。结果表明，四川小麦育成品种有14种HMW-GS，共23种组合类型，N/7+9/5+10组合出现频率最高，为12.30%；其次为N/7+8/2+12、N/7+9/2+12、1/6+8/2+12和N/7+8/5+10组合类型，出现频率分别为9.63%、9.09%、8.02%和8.02%（表3）。其他亚基组合类型频率较低，其中，1/14+15/5+10、1/22/5+10、1/23+18/2+12、1/6+8/3.1t+11*t、1/7/2+12、1/7/5+10和2*/7+9/5+10等7种类型只包含1份材料。表2四川省区试规定的小麦品质标准（2018年）Table 2Quality standards of wheat regional tests in Sichuan province（2018）类型Type弱筋小麦 Weak gluten wheat中筋小麦 Middle gluten wheat强筋小麦 Strong gluten wheat容重（g）Test weight770750770蛋白质含量（%）Protein content12.512.514.0湿面筋含量（%