常用荧光染料探针列表教学内容.doc

常用荧光染料探针列表精品文档这是来自于Salk的一个比较全的荧光染料列表，这些荧光染料可广泛用于流式细胞术以及荧光显微镜技术，汇集了各种荧光染料的特性，方便大家查找。可根据实际所用的检测平台、染料的最大激发光波长和最大发射光波长来选择合适的荧光染料用于实验。请注意这上面所显示的颜色可能会由于所用浏览器不同而有所不同，他们只是一个与实际颜色的近似值。染料名称Excitation(nm)Emission(nm)分子量备注信息Reactive and conjugated probesHydroxycoumarin325386331Succinimidyl esterAminocoumarin350445330Succinimidyl esterMethoxycoumarin360410317Succinimidyl esterCascade Blue(375);401423596HydrazidePacific Blue403455406MaleimidePacific Orange403551Lucifer yellow425528NBD466539294NBD-XR-Phycoerythrin (PE)480;565578240 kPE-Cy5 conjugates480;565;650670aka Cychrome, R670, Tri-Color, Quantum RedPE-Cy7 conjugates480;565;743767Red 613480;565613PE-Texas RedPerCP490675Peridinin chlorphyll proteinTruRed490,675695PerCP-Cy5.5 conjugateFluorX494520587GE HealthcareFluorescein495519389FITC; pH sensitiveBODIPY-FL503512TRITC547572444TRITCX-Rhodamine570576548XRITCLissamine Rhodamine B570590Texas Red589615625Sulfonyl chlorideAllophycocyanin (APC)650660104 kAPC-Cy7 conjugates650;755767PharRedAlexa Fluor系列荧光染料Alexa Fluor 350343442410Alexa Fluor 4054014211028Alexa Fluor 430434540702Alexa Fluor 488499519643QY 0.92Alexa Fluor 500503525700Alexa Fluor 514517542714Alexa Fluor 532530555724QY 0.61Alexa Fluor 5465615721079QY 0.79Alexa Fluor 5555535681250QY 0.1Alexa Fluor 568579603792QY 0.69Alexa Fluor 594591618820QY 0.66Alexa Fluor 6106106291285Alexa Fluor 6336326481200Alexa Fluor 6476526681300QY 0.33Alexa Fluor 6606636911100Alexa Fluor 6806807021150Alexa Fluor 7006967191400Alexa Fluor 7507527761300Alexa Fluor 7907828041750Cy系列荧光染料Cy2489506714QY 0.12Cy3(512);550570;(615)767QY 0.15Cy3B558572;(620)658QY 0.67Cy3.5581594;(640)1102QY 0.15Cy5(625);650670792QY 0.28Cy5.56756941128QY 0.23Cy7743767818QY 0.28核酸相关探针染料Hoechst 33342343483616 AT-selective DAPI345455AT-selective Hoechst 33258345478624AT-selective SYTOX Blue431480400DNA Chromomycin A3445575CG-selective Mithramycin445575YOYO-14915091271Ethidium Bromide493620394 Acridine Orange503530/640DNA/RNASYTOX Green504523600 DNA TOTO-1, TO-PRO-1509533Vital stain, TOTO: Cyanine DimerTO-PRO: Cyanine MonomerThiazole Orange510530Propidium Iodide (PI)536617668.4LDS 751543;590712;607472 DNA (543ex/712em), RNA (590ex/607em) 7-AAD5466477-aminoactinomycin D, CG-selectiveSYTOX Orange547570500 DNA TOTO-3, TO-PRO-3642661DRAQ5647681,697413usable excitation down to 488细胞功能探针Indo-1361/330490/4051010AM ester. Low/High Ca+,Fluo-3506526855AM ester. pH 6DCFH50553552927Dichorodihydrofluorescein, oxidized formDHR505534346Dihydrorhodamine 123, oxidized form,light catalyzes oxidationSNARF548/579587/635pH 6/9荧光蛋白QYBRPSY66H360442Y66F360508EBFP3804400.180.27monomerEBFP238344820monomerAzurite38344715monomerGFPuv385508T-Sapphire3995110.602625weak dimerCerulean4334750.622736weak dimermCFP4334750.401364monomerECFP4344770.153CyPet4354770.511859weak dimerY66W436485mKeima-Red4406200.243monomerTagCFP45848029dimerAmCyan14584890.7529tetramermTFP146249254dimerS65A471504Midoriishi Cyan4724950.925dimerWild Type GFP396,47550826k0.77S65C479507TurboGFP48250226 k0.5337dimerTagGFP48250534monomerS65L484510Emerald4875090.68390.69weak dimer; (Invitrogen)S65T488511EGFP48850726k 0.6034174weak dimerAzami Green4925050.7441monomerZsGreen1493505105k0.9140tetramerTagYFP50852447monomerEYFP51452726k0.615160weak dimerTopaz51452757monomerVenus5155280.575315weak dimermCitrine5165290.765949monomerYPet5175300.778049weak dimerTurboYFP52553826 k0.531.65dimerZsYellow15295390.6513tetramerKusabira Orange5485590.6031monomermOrange5485620.69499monomermKO5485590.6031122monomerTurboRFP55357426 k0.6762dimertdTomato5545810.699598tandem dimerTagRFP55558450monomerDsRed monomer55658628k0.13.516monomerDsRed2 (RFP)563582110k0.5524mStrawberry5745960.292615monomerTurboFP60257460226 k0.3526dimerAsRed2576592110k0.2113tetramermRFP158460730k0.25monomerJ-Red5846100.208.813dimermCherry5876100.221696monomerHcRed158861852k0.030.6dimerKatusha58863523dimermKate (TagFP635)58863515monomerTurboFP63558863526 k0.3422dimermPlum5906490.104.153mRaspberry5986250.1513monomer; faster photobleach than mPlum其他探针Monochlorobimane380461226Glutathione probeCalcein496517623pH 5Ex: Peak excitation wavelength (nm) Em: Peak emission wavelength (nm) QY: Quantum yield BR: Brightness; Extinction coefficient * Quantum yield / 1000 PS: Photostability; time to 50% brightness (sec) 光色波长(nm)代表波长 红（Red）780630700 橙（Orange）630600620 黄（Yellow）600570580 绿（Green）570500550 青（Cyan）500470500 蓝（Blue）470420470 紫（Violet）420380420收集于网络，如有侵权请联系管理员删除