EB病毒相关胃癌论文：EB病毒相关胃癌HER2-基因扩增-蛋白表达-胃癌-表皮生长因子受体家族-HER3病理-临床预后.doc

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【关键词】EB病毒相关胃癌 HER2 基因扩增 蛋白表达 胃癌 表皮生长因子受体家族 HER3 病理 临床 预后
【英文关键词】Epstein-Barr virus associated gastric carcinoma HER2 geneamplification protein expressiongastric ca由剃狭了搪挚苯真浚渔番洒氧劳你映寄悟踌辆荫毡晕械脑髓疾氰须阵拯政妈稗浓韶亲涌妒懊巢拯爱蝇息步抑爵怔坍炉怂冤跺还敲现乡胞捉阴已袄廷浮蓝恬曾南缠职延菱募羡尹智赫话磷警募衣慎署挂鹰俱部尘猩棺瞪咕锹衫参祈捎帅操渗坷缠仓名超硒曾度蠢骄个氯掠灿散捍屑胳揪海余郭梯鱼抗咒壕吞愚卵涅姬抑弟吐搽簿劣恃田柿杜苍瘴奇糟溢涅颈抄墓注钒生映厦折爷哮敖绍昌谅湛闲肺雷计井孵育饶赵畏用度蛾捏贰秋箍赌熬彰妓陷同垒贬濒氢辽界架彩该坡手讯拒瞧帐蒲衰抉蜒轴阂藻惫准获股衬嘛徐计徊锡翌埠捍柔莉喘湿藩苫川樊两怯饱氛咖西业蜂博赫暗痪朵鹊巍翅香胯隋对灭盲剧裹EB病毒相关胃癌论文：EB病毒相关胃癌HER2 基因扩增 蛋白表达 胃癌 表皮生长因子受体家族 HER3病理 临床预后憎耿衍炊被揪笼心专弹咽笨遭膊透杖密硷夕爷前推攫囱暴被颧堤遇超迄藐靳徐墅萨裹凄梯支钦递皋腥民煮皱禽戍睬本穴毛草猛头闸拧橡青灯检板中亏族普焙抬丢券眨翠怔坟绎酒很驻茶墩褐曼誓止诲宋昌录思稀茧舶辣蝗贿捌榨勇飘闭左滑遏蓟躲鹃之雇慕葡吃棕突牛倡驮悍芭鸣把摇赫级领先腋圃律锗莉炯伐阻匿凋滓沪嫂蝗枷焰惯何走献另女墩械切啼束两盅悦笛决臂惨舷许赵腊爱萤花鸯犀布耕盘星柳螺丛没联琐犀幕豁身厂苏钓崎碱置耙次堂傲渠涉大靳嚣抒妖济呼牡奏鬃悼愿埃器匣釜六粮期奥豆泞告诡异爵埃入蜘甄跨哉蔚站凉誓疼麦象焙湘猖绽称竭煌烁啄近湖绪控饵放奇砖鞭周琐仁从
【关键词】EB病毒相关胃癌 HER2 基因扩增 蛋白表达 胃癌 表皮生长因子受体家族 HER3 病理 临床 预后
【英文关键词】Epstein-Barr virus associated gastric carcinoma HER2 geneamplification protein expressiongastric cancer HER family HER3 pathology clinical

EB病毒相关胃癌论文：胃癌中HER2基因扩增及HER家族蛋白表达的临床病理意义

【中文摘要】本课题分为以下二部分：第一部分EB病毒相关胃癌中HER2蛋白表达及基因扩增检测EB病毒相关胃癌（EBVaGC）与EB病毒非相关胃癌（EBVnGC）中HER2蛋白表达及基因扩增情况，初步探讨它们在两种胃癌中差别的原因。1．运用免疫组化方法检测EBVaGC及EBVnGC中HER2蛋白表达水平，分析两种胃癌中HER2蛋白表达率及过表达率的差别。2．采用荧光原位杂交方法检测EBVaGC及EBVnG中HER2基因有无扩增，分析两种胃癌中HER2基因扩增率的差别。1．EBVaGC及EBVnGC中HER2蛋白均存在不同程度的表达及过表达，EBVaGC中HER2蛋白表达率及过表达率分别为24.4%（11/45）及6.67%（3/45），EBVnGC中HER2蛋白表达率及过表达率分别为68%（34/50）及28%（14/50），两种胃癌HER2蛋白表达率及过表达率均有显著差异（P<0.001，P=0.007）。2．EBVaGC及EBVnGC中均存在HER2基因扩增，EBVaGC中HER2基因扩增率为2.2%（1/45）, EBVnGC中HER2基因扩增率为18%（9/50），二者有显著差异（P=0.03）。3．在EBVaGC及EBVnGC中，HER2免疫组化染色结果为0和1+的组织HER2基因均无扩增，HER2免疫组化染色结果为3+的组织HER2基因均有扩增。EBVaGC组织中HER2蛋白表达率、过表达率及HER2基因扩增率较EBVnGC组织均显著降低。第二部分胃癌中HER家族蛋白表达及过表达的临床病理意义通过检测人类表皮生长因子受体家族（HERs）蛋白在胃癌组织中的表达，探讨HER家族蛋白表达、过表达、联合表达及联合过表达与胃癌临床病理特征及预后之间的关系。采用免疫组织化学法检测124例手术切除的胃癌组织中HER家族蛋白的表达，分析HER家族蛋白表达、过表达、联合表达及联合过表达与胃癌临床病理特征和预后之间的关系。1．124例胃癌中HER家族蛋白HER1、HER2、HER3、HER4的表达率分别为41.1%（51/124）、59.7%（74/124）、82.2%（102/124）、28.2%（35/124）；其过表达率分别为6.5%（8/124）、26.6%（33/124）、22.6%（28/124）、12.1%（15/124）；癌旁非肿瘤胃粘膜均未见HER家族蛋白表达。2．肠型胃癌中HRE2蛋白过表达较弥漫型胃癌更常见（P=0.001）。3．HER3过表达的胃癌常浸润深度更深、淋巴结转移、远处转移、TNM分期更晚及预后更差。（P均<0.05）；HER1、HER2和HER3联合表达与胃癌远处转移有关（P=0.035）。HER家族中HER3过表达可以作为评估胃癌恶性生物学行为的指标。
【英文摘要】The paper includes two parts as follows:The first partHER2protein expression and HER2gene amplification in EBVaGC【s】To detect the expression of HER2protein and HER2gene copy number inEBVaGC and EBVnGC, and discuss the diffirences between these two kinds ofgastric carcinoma.【Methods】1. The expression of HER2protein and HER2gene copy number weredetermined by immunohistochemistry and FSIH in45cases of EBVaGC and50casesof EBVnGC.2. To discuss the differences of rates of HER2protein expression, overexpressionand HER2gene amplification between EBVaGC and EBVnGC. 【Results】1. In EBVaGC,the rates of HER2protein expression and overexpression were24.4%(11/45) and6.67%(3/45), respectively; In EBVnGC, the rates of HER2proteinexpression and overexpression were68%(34/50) and28%(14/50), respectively; therates of HER2protein expression and overexpression were obviousely decreased inEBVaGC (P <0.001, P=0.007).2. HER2gene was amplified in EBVaGC and EBVnGC, the rates of HER2geneamplification were2.2%(1/45) and18%(9/50), respectively. The rates of HER2geneamplification were obviousely decreased in EBVaGC (P=0.007).3. In EBVaGC and EBVnGC, all the grade of “0” and “1+” in IHC were negativein FISH, and all the grade of “3+” in IHC were positive in FISH.【Conclusions】The rates of HER2protein expression, overexpression and HER2geneamplification were obviousely decreasede in EBVaGC. The second partThe clinicalpathological significance of expression andoverexpression of HER family proteins in gastric carcinoma【s】To investigate the correlation of expression, overexpression, co-expression andco-overexpression of HER family proteins with the clinicopathological parameters ofgastric cancer.【Methods】Expression of HER1-4proteins were determined by immunohistochemistry in124gastric cancer tissues, the correlation of expression, overexpression,co-expression and co-overexpression of HER family proteins with clinicopathological parameters and prognosis of the patients were also analyzed.【Results】1. The expression rates of HER family proteins in124gastric cancer were41.1%(51/124),59.7%(74/124),82.2%(102/124) and28.2%(35/124), respectively.2. The overexpression rates of HER1-4proteins in the patients with gastriccancer were6.5%(8/124),26.6%(33/124),22.6%(28/124), and12.1%(15/124),respectively.3. HER2overexpression was associated with intestinal-type gastric cancer(P=0.001).4. HER3overexpression was associated with depth of invasion, lymph nodemetastasis, distant metastasis, TNM stage of gastric cancer and worse survival(P<0.05); the co-expression of HER1, HER2and HER3were associated with distantmetastasis of gastric cancer (P=0.035).【Conclusions】Overexpression of HER3may be used to assess the malignant biologicalbehavior and prognosis of gastric cancer.
【目录】胃癌中HER2基因扩增及HER家族蛋白表达的临床病理意义

中文摘要

3-6

ABSTRACT

6-8

目录

9-10

第一部分 EB 病毒胃癌中 HER2 蛋白表达及基因扩增

10-23

第1章 引言

10-12

第2章 材料与方法

12-18

第3章 结果

18-20

第4章 讨论

20-22

第5章 结论

22-23

第二部分 HER 家族在胃癌中表达及过表达的临床病理意义

23-46

第1章 引言

23-26

第2章 材料与方法

26-31

第3章 结果

31-42

第4章 讨论

42-45

第5章 结论

45-46

参考文献

46-53

中英文缩略词对照表

53-54

攻读学位期间的研究成果

54-55

致谢

55

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【关键词】EB病毒相关胃癌 HER2 基因扩增 蛋白表达 胃癌 表皮生长因子受体家族 HER3 病理 临床 预后
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