医学模式图素材.ppt

单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,*,*,Antibody Toolkit(,IgG,),helix,Vimentin,Actin,and myosin,Coiled-coil,Microtubule,6 nm,10 nm,25 nm,Nucleosomes,2,AC,AC,DD,R1,DD,R1,DD,R1,7,6,1,2,3,4,5,4,3,5,6,2,1,7,CD45,PTP,Metabolism of 5-fluorouracil(5-FU),5-FU,Carac,Efudex,5-Fluorouridine,5-FUMP,5-FdUMP,Uridine,phosphorylase,5-Fluorodeoxyuridine,Thymidine,phosphorylase,Orotate,phosphoribosyl,transferase,Thymidine,kinase,5-FUTP,5-FdUTP,RNA,DNA,5-nucleotidase,5-nucleotidase,Uridine,kinase,ATP,ADP,ATP,ADP,PRPP,Western Blotting,1.Resolve protein samples on SDS-PAGE,6.Incubate the blot with,chemiluminescent,HRP substrate and expose to film,2.,Electrophoretically,transfer fractionated proteins from gel onto PVDF membrane,3.Block the membrane with neutral protein (BSA or milk casein),4.Incubate the membrane with primary antibody specific to target protein,5.Incubate the membrane with HRP-labeled secondary antibody specific to primary antibody,