工业化生物发酵技术——清华大学国际技术转移中心.doc

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Biotechnology Catalogue Version 201306 Note: Most of the following technologies are for A.P.I.s, and they have been successfully commercialized in the market. www.coway- 工业化生物发酵技术（原料药） Product名称 Guaranteed Values指标 ACARBOSE阿卡波糖 Final titre: 8-9g/lt Fermentation time: 120-144hrs Recovery yield: 55% ACLARUBICIN 阿柔比星 Two step fermentation ACRYLAMIDE 丙烯酰胺 Final titre: 325 g/lt Fermentation time: 40-48 hrs Recovery yield: 98% AMOXICILLIN 阿莫西林 Yield: ＞90%(guaranteed; semi-continuous process with recycling) ＞95%(achieved; semi-continuous process with recycling) Amphotericin B两性霉素B Final titre: 10000U/ml(HPLC) Fermentation time: 130-140hrs Recovery yield: 70% ANDROSTENDIONE-SPIRONOLACTONE 雄烯二酮-螺内酯 Androstendione Final titre: 20 ± 2 g/lt Fermentation time: 96 ± 24 hrs Recovery yield: >90 % as Crystals 96% purity Sprironolactone Synthesis: 66% from AD APRAMYCIN Sulfate 硫酸安普霉素 Final titre: 8 g/l Fermentation time: 144 ±12 hrs Recovery yield: ≥80% ARACHIDONIC ACID 花生四烯酸 Final titre: 10 ±1 g/lt Fermentation time: 134 hrs Recovery yield: 80 ± 5% ARGININE精氨酸 Final titre: 75±5g/L Fermentation time: 50 hrs Recovery yield: >80％ as crystal >90％ feed grade ASTAXANTHIN 虾青素 Final titre: 3 g/lt Fermentation time: 180±10 hrs Recovery yield: 60% AVERMECTIN阿维菌素 Final titre: 5000 u/ml (+ 10%) Fermentation time: 260-280 hrs Recovery yield: > 75% BACITRACIN-Zinc 杆菌肽锌 Final titre 1200u/mL Fermentation time: 36 hrs Recovery yield: ≥55 % BLEOMYCIN 博莱霉素 Final titre: 140±20mg/L Fermentation Time: 144±12hrs Recovery yield: ≥35% CABERGOLINE卡麦角林 By chemical synthesis from Lysergic acid 3.29kg Cabergoline from 1kg Lysergic acid b-CAROTENE b-胡萝卜素 Final titre: 3g/l Fermentation time: 96±12 hrs Recovery yield as feed quality: 90 % CEPHALOSPORIN C, Na SALT 头孢菌素C钠盐 Final titre: 28 g/lt Pilot plant titer: > 30 g/lt Fermentation time: 144±24 hrs Recovery yield: >80% CHLORTETRACYCLINE金霉素 Final titre: 33 g/L Fermentation time: 100 hrs Recovery yield: 94 % feed grade, 75 % hydrochloride Citric Acid柠檬酸 The yield，conversion rate and fermentation time are different between using different kinds of raw materials. CLAVULANIC ACID 克拉维酸 Final titre: 11±1g/lt Fermentation time: 140 ± 12 hrs Recovery Yield: 60±5 % COMPACTIN美伐他汀 Final titre: 14 g/l CYCLOSPORIN A 环孢霉素A Final titre: 5 ± 1 g/lt Fermentation time: 168 hrs Recovery yield: 70 % DALBAVANCIN PRECURSOR 达巴万星（道古霉素）前体 Final titre: 1 ± 0.2 g/lt Fermentation time: 216±12 hrs Recovery yield: ≥50 % DAUNOMYCIN 柔红霉素 Final titre: 3g±0.3g/lt Fermentation time: 168±12 hrs Recovery yield: ≥67 % DAPTOMYCIN/CUBICIN 达托霉素 Final titre: 2.5±0.3 g/lt Fermentation time: 196±24hrs Recovery yield Pharma grade: ≥40% DESFEROXAMIN 去铁胺 Final titre: 15 ±1.5 g/lt Fermentation time: 192±12 hrs Recovery yield: 35 - 40 % DHA Final titre: 28 ±3 g/lt Fermentation time: 120±24 hrs Recovery yield: >80 % DMCT/Demethylchlortetracycline去甲基氯四环素/去甲金霉素 Final titre: 10.0 ± 1g/L Fermentation time: 300 hrs Recovery Yield: 75％ DORAMECTIN 多拉霉素 Final titre: 1500UI/ml Fermentation time: 200 hrs Recovery Yield: 75％ DOXORUBICIN阿霉素 Antitumor drug Chemical synth. from Dauno: Yield >50% ENRAMYCIN 恩拉霉素 Final Titre: 10g/lt Fermentation Time: 260hrs Recovery yield: ≥92% ERYTHROMYCIN 红霉素 Final titre (HPLC): 10 ± 0.5g/lt Fermentation time: 168+24 hrs Recovery yield: 85% as Thiocyante. 82% as Base EPIRUBICIN表阿霉素 Antitumor drug Chemical synth. from Dauno: Yield >30% EPOTHILONE B 埃博霉素B Final titre: 70-90 mg/L Fermentation time: 168-216 hrs FLAVOMYCIN黄霉素 Final titre: 14 ±1 g/lt Fermentation time: 148 - 172 hrs Recovery yield as feed quality: >92 % Fusidic Acid 福西地酸 Final Titre: 3.8g/L Fermentation Time: 190hrs Recovery yield: ≥60% FRUCTO-OLIGO SACCARIDE (FOS) 果寡糖 Contact us for more details GENTAMYCIN 庆大霉素 Final titre: 6g/l Fermentation time: 14days Recovery yield: 80% Glutathione(GSH) 谷胱苷肽 Final titre: 5.5±0.5 g/l Fermentation time: 65±5 hrs Recovery yield: 45-50% GUANOSINE 鸟苷 Final titre: 50-60 g/l Fermentation time: 72 hrs Sugar conversion rate: 25% Recovery yield: 80% GIBBERELLIN (GA) 赤霉素 Fermentation loading coefficient：90—95% Fermentation titre： mix constituents：2200-2400μg/ml； GA3 constituents：1900—2100μg/ml Extraction yield：85% HYALURONIC ACID透明质酸 Final titre: 6.5 g/litre Fermentation time: 20-24 hrs Recovery yield: 90 % Cosmetic Grade INOSINE 肌苷 Final titre: ≥90 g/l Fermentation time: 85-90 hrs Sugar conversion rate: 35% Recovery yield: 80% KANAMYCIN 卡那霉素 Final titre: 30g/l Fermentation time: 300 hrs Recovery yield: 80% KASUGAMYCIN 春雷霉素 Final titre: 10000-12000U/ml Fermentation time: 8 days Recovery yield: > 80% LACTIC ACID 乳酸 Final titre: 120 ±10g/lt Fermentation time: 72 ± 10 hrs Recovery yield: >90% LINCOMYCIN 林肯霉素 Final titre: 8 g/lt Fermentation time: 192 ± 24 hrs Recovery yield： Pharma grade 80% Feed Grade 90% LIPSTATIN 泥泊司他汀 Chemical conversion from Lip to Orlistat ORLISTAT 奥利司他 Final titre: 8 ± 1 g/lt Fermentation time: 168 hrs Recovery yield: 75% Contact us for more details. LOVASTATIN 洛伐他汀 Final titre 17g/lt Fermentation time 264 ± 24 hrs Recovery yield 70 ± 5 % Lycopene番茄红素 Titre： 2.5 ± 0.5 g/l Fermentation time: 120±12 hrs Extraction yield: 75±5% LYSERGIC ACID 麦角酸 Final titre 6 g/l Fermentation time: 360 hrs Recovery yield: ≥ 70 % LYSINE 赖氨酸 Final titre: 190g/l Fermentation time: 45 hrs Recovery yield: 95 % for crystal (assay >98.5%) 100% for feed grade (assay 65%) Sugar conversion rate: 55% - 60 % MADURAMYCIN 马杜拉霉素 Final titre: 10’000 – 12’000 UI/ml Fermentation time 180 – 210 hrs. Recovery yield: > 90 % Feed Grade MIBEMYCIN 美贝霉素 Final titre: ≥400 U/ml Extraction yield: 60%-70% Synthetic yield: 30%-50% MITHRAYCIN光辉霉素 Production Titre： > 500 mg/l MONENSIN 莫能菌素 Final titre: 32000-40000 u/ml Fermentation time: 320 hrs. Recovery yield: 95% MUPIROCIN 莫匹罗星 Final Titre： 3000mg/L（50L fermentor） Fermentation Time： 60hrs MYCOPHENOLIC ACID 麦考酚酸 Final titre: 16±1 g/l Fermentation time: 312±24hrs Recovery yield: > 68 % NATAMYCIN 纳他霉素 Final titre: 8g/l(Maximum 10g/l) Fermentation time: 140-160 hrs. Recovery yield: 55-65% NICERGOLINE 尼麦角林 By chemical synthesis from Lysergic acid 2.47kg nicergoline from 1kg Lyserig acid NYSTATIN制霉菌素 Final titre: ＞400,000 IU/ml Fermentation time: 324±24 hrs. Recovery yield: 85 % Feed Grade, 70 % Pharma Grade OXYTETRACYCLINE土霉素 Final titre: 33mg/l Fermentation time: 240hrs Recovery yield feed grade: 94% PARAMOMYCIN巴龙霉素 Final titre: 6g/lt Fermentation time: 156 hrs Recovery yield: 70 % as Crystals PENICILLIN G POTASSIUM SALT 青霉素G钾盐 Final titre: 80,000 IU/ml Fermentation time: 192 hrs Recovery yield: ³ 85 % Phenylalanine 苯丙氨酸 Final titre: 32±2g／l Fermentation time: 45-55 hrs Recovery yield: >75％ Conversion rate: 14-16％ PLEUROMUTILIN截短侧耳素 Final titre: 13 g/lt（50L fermenter） Pneumocandin Bo 肺囊康定 New Chemical conversion from Bo to caspofungin CASPOFUNGIN 卡泊芬净 Final titre: 2.3-3.0g/L Fermentation time: 312 hrs Recovery yield: 70 % Contact us for more details. MICAFUNGIN 米卡芬净 Contact us for more details ε-Polylysine 聚赖氨酸 Yield of acid by fermentation: 20g/l Fermentation time： 70hrs Recovery yield： 80％ POLYMYXIN 粘杆菌素B Final titre: 2.5 ±0.2 g/l Fermentation time: 168±24 hrs Recovery yield: >70 % POLYMYXIN E Sulfate硫酸粘杆菌素E Final titre: 450.000 U/l (Maximum 560,000U/l) Fermentation time 96 hrs±10 Recovery yield: 93-95% concentrate 80-85% A.P.I. PRAVASTATIN 普伐他汀 Titre: 15 g/l Fermentation time: 9-10days Recovery yield: >65 % Conversion from Mevastatin : > 70% Prednisolonum/ Prednisolone 氢化泼尼松/泼尼松龙 Concentration of substrate: >20 g/L (Maximum 70g/L) Bioconversion time: 5-8 hrs Substrate conversion rate: 97～100％ Yield of crystalline final product: >80％ PRISTINAMYCINS 普那霉素/原始霉素 Final titre: 2.5- 3.0g/L Fermentation time: 64 hrs. Recovery yield: 75% COENZYME Q10 辅酶Q10 Final titre: 3.5±0.5g/l Fermentation time: 120±12 hrs Recovery yield: >50 % Ramoplanin雷莫拉宁 Final titre: 2±0.2 g/L Fermentation time: 216±12 hrs Recovery yield: ≥50% RIFAMYCIN-RIFAMPICIN 利福霉素-利福平 Final titre: > 26 g/lt Fermentation time: 192 hrs Recovery (up to Rifampycin): >65% Rh- LACTOFERRIN 乳铁蛋白 Final titre: 3.5±0.3g/L Fermentation time: 168±12hrs Recovery yield: 60±5% SAMe 腺苷蛋氨酸 Final titre: ≥10g/L Fermentation time: 16 hrs SALINOMYCIN 盐霉素 Final titre: 10 g/lt Fermentation Time: 360 hrs ± 24 Recovery as Feed Grade: > 92 % SHIKIMIC ACID 莽草酸 Final titre: 30 ± 3 g/lt Fermentation time: 60 ±12 hrs Recovery yield: 75% SIMVASTATIN 辛伐他汀 Chemical conversion of Lovastatin 11 – 12 kg Simvastatin from 20 kg Lovastatin SIROLIMUS 西罗莫司 Final titre: 1.2 ± 0.1 g/lt Fermentation time: 216 ±24 hrs Recovery yield: >50% SPECTINOMYCIN 大观霉素 Final titre: 4000u/ml Recovery yield: 65-70 % SPIRAMYCIN/Rovamycin 螺旋霉素 Final titre: 5000r/m Fermentation Time: 144 hrs Recovery yield: 76% STREPTOMYCIN 链霉素 Final titre: 24 g/l Fermentation time: 200 hrs Recovery yield: 72 % TACROLIMUS 他克莫司 Final titre: 1.2 g/l Fermentation time: 240 + 24hrs Recovery yield: > 70 % TEICOPLANIN替考拉宁 Final titre: 5+ 0.5 g/l Fermentation time: 168 + 12 hrs Recovery: ³ 55 % Testosterone睾丸激素 Concentration of substrate: 5.0 g/L Bioconversion time: 135～145 hrs content in cultivation broth: 48～50％ Yield of crystalline final product: 30～35％ TETRACYCLINE HCI 盐酸四环素 Final titre: >30 g/l Fermentation time: 200 hrs Recovery yield: 74 % THREONINE苏氨酸 Final titre: 85-95 g/l Fermentation time: 35-40 hrs Recovery: 80% THIAMULIN Base泰妙菌素碱 Conversion rate: From 1 kg Pleuromutilin to 1,23 kg Thiamulin Fumarate TOBRAMYCIN 妥布霉素 Final titre: 6000r/ml Fermentation time: 120-144 hrs Recovery yield: 30 % TRYPTOPHAN 色氨酸 Final titre: 35±3 g/L Fermentation time: 50±5 hrs Recovery yield as crystal: ≥70% Recovery yield as Feed Grade: ≥85% Glucose conversion rate: 12-15％ TYLOSIN TARTRATE 酒石酸泰乐菌素 Final titre: 15± 1 g/lt. Fermentation time: 180±12 hrs Recovery yield: ≥70% VALINE 缬氨酸 Final titre: 60±5 g/l Fermentation time: 60 ± 5 hrs Recovery yield Pharma grade: ≥65% VANCOMYCIN 万古霉素 Final titre: 12 ± 1 g/l Fermentation time: 180 ± 12hrs Recovery yield: 70 % VITAMINE B12 维生素V B12 Final titre: 220 g/L Fermentation time: 168 hrs Recovery yield: > 70% as Crystal, 80% as Feed Grade VITAMINE B2 Final titre: 33 ± 3g/l Fermentation time: 48 ± 24 hrs Recovery yield: 65 % as Pharma Grade, 80 % as Feed Grade XANTHAN 黄原胶 Final titre: 25 kg/m3 Recovery yield: 80 % Fermentation Time 36 hrs AMPICILLIN氨苄青霉素 (CAS 69-53-4) Bio-Enzymatic Reaction CEPHALEXIN头孢氨苄(CAS 15686-71-2) Bio-Enzymatic Reaction CEPHADROXIL 头孢羟氨苄(CAS 50370-12-2) Bio-Enzymatic Reaction D-p-OH-Phenylglycine-amide Bio-Enzymatic Reaction D-p-OH-Phenylglycine-ester Bio-Enzymatic Reaction L-tert-leucine L-亮叔氨酸 Biocatalytic method L-homophenylalanine L-高苯丙氨酸 Biocatalytic method TECHNOLOGIES OF ENZYMES PRODUCTION （HIGH） ALKALINE PROTEASE 碱性蛋白酶 Final titre: 30000 U/ml Fermentation time: 50 hrs Cellulase 纤维素酶 Activity of enzyme in harvest broth when release: 60-80 IU/ml Fermentation time: 140-150 hrs Recovery yield: 85－90％ Dextranse 葡聚糖酶 Fermentation titre： 10000 U/ml flask 1000u/ml Heparanase 肝素酶 Heparanase activity: 20000IU/L Fermentation time: 24±5 hrs Recovery yield: 65% Lipase脂肪酶 Fermentation titre： 1500－2000 FIPU/ml Fermentation Time： 72－96 hrs Recovery yield： 70－90 PENICILLIN ACYLASE 青霉素酰化酶 Enzyme activity: 250-260 u/g (28°C dry basis) Final titre: 50-60u/ml (28°C) Fermentation time: 120-140 hrs Recovery yield: ≥40% THERMOSTABILITY AMYLASE 耐高温淀粉酶 Fermentation time: 150hrs Activity: 100,000,000 U/mL GLUCOAMYLASE 葡糖淀粉酶 Fermentation time: 180hrs Activity: 100,000 U/mL Xylanase木聚糖酶 Incision enzyme activity: 120U/ml Excision enzymeactivity: 60－80U/ml Fermentation time: 72 hrs Recovery yield: 70－

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