戴安 WPS-3000维修手册.pdf

DIONEXWPS-3000Nan/Cap Well Plate Microautosamplerfor the UltiMate 3000 SystemService InstructionsRevisio n:VI.0Date:Jul y 1,2006 2006 DionexWPS-3000 Series Well Plate Nan/Cap Microautosampler Service InstructionsTable of Contents1 Introduction.11.1 Ho w to use these Servic e Instruc tio ns.11.2 Ad d itio nal Do c uments.21.3 General Info rmatio n.21.4 Instruc tio ns fbr Returning Instruments o r Parts.32 Preparatory Measures.42.1 General Care and Prec autio ns.42.2 Safety Prec autio ns.43 Identifying the Instrument Version.53.1 Avail abl e Co nfiguratio ns.53.2 Instrument Mo d ific atio ns.54 Electrical Connection.65 Injection System and Principle.75.1 Operating Princ ipl e.75.2 Injec tio n Princ ipl es.85.3 Sel ec ting the pro per Syringe,Buffer Tubing and Injec tio n Lo o p.86 Instrument Checkout.96.1 Chec king the Po sitio n o f the Need l e Pair.106.2 Chec king Po wer Suppl y Vo l tages and Signal s.116.2.1 Vo l tages and Signal s o f the Po wer Suppl y Bo ard(S/N 2620601).126.3 Chec king Main Bo ard Vo l tages and Signal s.136.3.1 Vo l tages and Signal s o f the Main Bo ard(S/N 2620601).156.4 Chec king the Mo to r Driver Vo l tages.177 Chromeleon Service Functions.187.1 Ul tiMate 3000 Servic e Panel.187.2 The Auto sampl er Servic e Panel s.187.2.1 The Sampl erChange Panel.207.2.2 The Sampl erAl ignment Panel.207.3 Servic e Level Co mmand s and Pro perties.237.3.1 Chro mel eo n Servic e Mo d e.237.3.2 Co mmand s and Pro perties o f the Devic e Sampl er.247.4 Servic e Co d e and Servic e Mo d e.328 Maintenance and Repairs.338.1 Repl ac ing the Fuses.348.2 Repl ac ing the Syringe.358.2.1 Remo ving the Syringe.358.2.2 Instal l ing the Syringe.368.3 Repl ac ing the Buffer Tubing.378.4 Repl ac ing the Sampl e Lo o p.388.5 Repl ac ing the Syringe Val ve.39WPS-3000 Series Well Plate Nan/Cap Microautosampler Service Instructions8.6 Repl ac ing the Need l e Pair(Punc turer and Sampl e Need l e).408.6.1 Repl ac ing the Need l e.408.6.2 Repl ac ing the Punc turer.418.7 Repl ac ing the Injec tio n Val ve.428.8 Remo ving and Instal l ing the Sid e Panel s and the To p Co ver.438.9 Remo ving and Instal l ing the Rear Panel.468.9.1 Remo ving the Rear Panel(No Co o l ing Optio n instal l ed).468.9.2 Remo ving the Rear Panel(Co o l ing Optio n instal l ed).468.10 El ec tro nic s Unit.478.10.1 Repl ac ing the El ec tro nic s Unit(S/N 2620601).498.10.3 Repl ac ing the Po wer Suppl y Bo ard(S/N 2620601).538.10.4.A Safety Test for the Switching Power Supply Unit.548.10.5 Repl ac ing the Main Bo ard(S/N 2620601).578.10.6.A Replacing the Fuse on the Main Board(S/N 2620601).598.10.7 Injec tio n Val ve Co ntro l Mo d ul e.608.11 Peristal tic Pump.618.11.1 Repl ac ing the Cartrid ge.618.11.2 Repl ac ing the Peristal tic Pump.618.12 Repl ac ing the Leak Senso r.628.13 Repl ac ing the Do sage Unit.638.14 Repl ac ing the Mo to r/Gear Bo x Assembl y.648.14.1 Remo ving the Mo to r/gear Bo x.648.14.2 Instal l ing the Mo to r/gear Bo x.648.15 Repl ac ing the Ho rizo ntal Drive.658.16 Repl ac ing the Heat Exc hanger.668.16.1 Remo ving the Heat Exc hanger.668.16.2 Instal l ing the Heat Exc hanger.678.17 Repl ac ing the Displ ay/Keybo ard Assembl y.689 Calibration and Adjustment Procedures.699.1 Displ ay Brightness and Co ntrast Limits.709.2 Leak Senso r Sensitivity.729.3 Al ignment o f the Need l e,Wash Po rt and Tray Po sitio n.739.3.1 Preparatio ns.739.3.2 Tray Po sitio n.759.3.3 Wash Po rt.769.3.4 Need l e Height.7710 Troubleshooting.7910.1 Hard ware Pro bl ems.7910.2 Erro r Messages o n the Fro nt Panel.8010.3 Erro r Messages in the Chro mel eo n Aud it Trail.8511 Firmware Download.8712 Spare Parts Lists.88iiWPS-3000 Series Well Plate Nan/Cap Microautosampler Service Instructions13 Technical Appendix.9313.1 Maintenanc e o f the Injec tio n Val ve.9313.1.1 Cl eaning and Repl ac ing Parts.9313.1.2 Disassembl y o f the Val ve.9313.1.3 Reassembl y o f the Val ve.9413.2 Pin Out.9513.3 El ec tric al Bl o c k Diagram.96mWPS-3000 Series Well Plate Nan/Cap Microautosampler Service InstructionsA Important:Warning:1 Introduction1.1 How to use these Service InstructionsThis manual enabl es yo u to effec tivel y so l ve pro bl ems that may o c c ur with the WPS-3000 Series Nano/Cap Well Plate Microautosampler.Befo re perfo rming any repair o r maintenanc e pro c ed ures,pl ease read the Operating Instructions and Service Instructions tho ro ughl y to o btain a general id ea o f the instrument.Note:The servic e manual is intend ed o nl y fo r Dio nex(o r autho rized)servic eperso nnel to assist in the maintenanc e and repair o f Ul tiMate 3000 series instruments and sho ul d no t be mad e avail abl e to o ther ind ivid ual s.Note:It is assumed that the ind ivid ual using this manual has suffic ient training inthe servic e o f anal ytic al instrumentatio n and is aware o f the po tential hazard s inc l ud ing(but no t l imited to)el ec tric al hazard s,c hemic al so l vent hazard s,expo sure to UV rad iatio n and the expo sure to pressurized so l vents.The servic e manual is intend ed as a suppl ementary guid e to the Operating Instructions fo r q ual ified servic e engineers.Fo r the l atest info rmatio n,al so see the Product Support Bulletins(=PSB)fo r the WPS-3000 Series Nano/Cap Wel l Pl ate Mic ro auto sampl er.Dio nex c anno t be hel d l iabl e fo r any repairs o r maintenanc e wo rk c arried o ut by unq ual ified perso nnel.Al mo st al l d esc riptio ns in the manual appl y to al l mo d el s o f the WPS-3000 Series Nano/Cap Wel l Pl ate Mic ro auto sampl er and c o ver bo th the stand ard(stainl ess steel)and the bio c o mpatibl e versio n.Therefo re,the term the auto sampl er is used thro ugho ut the manual.If so me d etail appl ies to o nl y o ne mo d el o r versio n,the mo d el(versio n)is id entified by name.Note:The d evic e c o nfiguratio n may vary;therefo re,no t al l d esc riptio nsnec essaril y appl y to yo ur partic ul ar instrument.Note:The d esc riptio ns in this manual refer to firmware versio n 1.11 andChro mel eo n 6.70.At vario us po ints thro ugho ut the manual,messages o f partic ul ar impo rtanc e are ind ic ated by the fo l l o wing symbo l s:Ind ic ates general info rmatio n intend ed to o ptimize the servic e and maintenanc e steps o r the perfo nuanc e o f the instrument.Ind ic ates that fail ure to take no te o f the ac c o mpanying info rmatio n may resul t in d amage to the instrument.Ind ic ates that fail ure to take no te o f the ac c o mpanying info rmatio n may resul t in perso nal injury.Tip:WPS-3000 Series Well Plate Nan/Cap Microautosampler Service InstructionsThe info rmatio n c o ntained in this manual is subjec t to c hange witho ut no tic e and sho ul d no t be c o nstrued as a c o mmitment by Dio nex.Dio nex assumes no respo nsibil ity fo r any erro rs that may appear in this d o c ument.This d o c ument is bel ieved to be c o mpl ete and ac c urate at the time o f publ ic atio n.In no event shal l Dio nex be l iabl e fo r inc id ental o r c o nseq uential d amages in c o nnec tio n with o r arising fro m the use o f this d o c ument.CHROMELEON is a registered trad emark o f Dio nex Co rpo ratio n.Al l o ther trad e o r c o mpany names mentio ned are subjec t to the c o pyright and the pro perty and trad emark rights o f the respec tive c o mpany.Al l rights reserved,inc l ud ing tho se fo r pho to mec hanic al repro d uc tio n and sto rage o n el ec tro nic med ia.No part o f this publ ic atio n may be c o pied o r d istributed,transmitted,transc ribedsto red in a retrieval system,o r transmitted into any human o r c o mputer l anguage,in any fo rm o r by any means,el ec tro nic,mec hanic al,magnetic,manual,o r o therwise,o r d isc l o sed to third parties witho ut the express written permissio n o f Dio nex Co rp.1.2 Additional DocumentsIn ad d itio n to the servic e instruc tio ns,the fo l l o wing d o c uments are avail abl e:Operating Instruc tio ns Warranty Return Fo rm and Servic e Return Fo rm(inc l ud ing Dec o ntaminatio n Certific ate)OQ/PQ Instruc tio ns Spare Parts Catal o g Pro d uc t Suppo rt Bul l etins SOP Perfo rming Maintenanc e o f Ul tiMate 3000 HPLC Systems1.3 General InformationThe WPS-3000 Series Nano/Cap Wel l Pl ate Mic ro auto sampl er is mad e o f high-q ual ity c o mpo nents and material s whic h keep maintenanc e req uirements to a minimum.A Important:Use o riginal Dio nex spare parts o nl y.Substituting no n-Dio nex parts may impair the perfo rmanc e o f the instrument,thereby vo id ing the pro d uc t warranty.In ad d itio n,Dio nex assumes no l iabil ity fbr d amage c aused by repairs with third-party parts.The painted surfac es are rel ativel y resistant to weak ac id s and to al kal ine o r o rganic so l utio ns.Neverthel ess,al l so l vents o r o ther l iq uid s spil l ed o nto the surfac e sho ul d be wiped up immed iatel y,using a l int-free c l o th o r tissue(avo id rubbing).Warning:Maintenanc e and/o r repair wo rk sho ul d be c arried o ut by q ual ified perso nnel o nl y.When o perating the auto sampl er with the enc l o sure o pen,spec ial c autio n sho ul d be taken bec ause o f the l ine po tential.In any c ase,o bserve the warning no tes in the servic e instruc tio ns2WPS-3000 Series Well Plate Nan/Cap Microautosampler Service InstructionsWarning:The fl uid c o mpo nents o f the auto sampl er may be fil l ed with to xic so l vents.Befo re starting maintenanc e o r repair wo rk,rinse the fl uid ic s with a suitabl e so l vent and put o n appro priate pro tec tive c l o thing.Befo re yo u start wo rking,c hec k the fuses fbr the c o rrec t rating and repl ac e,if appro priate(f Repl ac ing the Fuses,page 34).A Important:When turning o n the unit fo r test purpo ses,o bserve the instal l atio n instruc tio ns in the Operating Instructions.The auto sampl er is shipped in a c o ntainer that is spec ial l y d esigned to pro tec t the unit d uring shipping.If it is nec essary to return the unit to the fac to ry(via yo ur Dio nex subsid iary o r d istributo r),pl ease make c ertain that it is returned in this shipping c o ntainer and o bserve the stand ard RMA pro c ess(fsec tio n 1.4,page 3).If the unit is no t shipped in the o riginal pac king(f Spare Parts Lists,page 88),the pro d uc t warranty wil l no t be ho no red.1.4 Instructions for Returning Instruments or PartsTo return any instrument o r part fbr repair o r fo r exc hange und er warranty,return it to yo ur Dio nex subsid iary(o r d istributo r).They wil l return the instrument o r part to the fac to ry,o bserving the stand ard RMA(return material autho rizatio n)pro c ess.The pro c ess c o ntains the fo l l o wing steps:Req uest an RMA(Return Material Autho rizatio n)number fro m Dio nex So ftro n at.rmaso ftro n.d e Ind ic ate the RMA number o n the ad d ress l abel o f the pac kage and al l d o c uments attac hed to the c o nsignment.This hel ps us to pro perl y trac k and ac c o unt fo r the instrument.Fil l in the Servic e Return Fo rni and enc l o se it with every instrument o r part returned to the fac to ry.Al so fil l in and enc l o se the Warranty Cl aim Fo rm if nec essaryA c o py o f bo th d o c uments is inc l ud ed in the Operating Instructions fbr the instrument.(In ad d itio n,the fo rms have been d istributed with a pro d uc t suppo rt bul l etin(PSB).)A Tip:The Servic e Return Fo rm inc l ud es a Dec o ntaminatio n Certific ate.Fo r safety reaso ns,we c anno t start repairing the instrument o r the part if the Servic e Return Fo rm is missing o r if the Dec o ntaminatio n Certific ate is no t c o mpl eted.Make c ertain that a c o py o f the Dec o ntaminatio n Certific ate is attac hed to the o utsid e o f the pac kage.Careful l y pac k the instrument,o bserving the pac king instruc tio ns and preferabl y using the o riginal shipping c o ntainer,and ship it to the ad d ress bel o w:Dio nex So ftro n GmbHDo rnierstrasse 4D-82110 GermeringGermanyA Tip:The pac king instruc tio ns are avail abl e fro m Dio nex So ftro n o n req uest.3WPS-3000 Series Well Plate Nan/Cap Microautosampler Service Instructions2 Preparatory Measures2.1 General Care and PrecautionsThe WPS-3000 Series Nano/Cap Wel l Pl ate M i c ro auto samp I er is mad e o f high-q ual ity c o mpo nents and material s whic h keep maintenanc e req uirements to a minimum.The painted surfac es are rel ativel y resistant against weak ac id s and al kal ine and o rganic so l vents.Neverthel ess,immed iatel y wipe up al l l iq uid s spil l ed o nto the instruments surfac e,using a l int-free c l o th o r paper(avo id rubbing).If surfac es are expo sed fbr l o nger perio d s,these l iq uid s c an c ause d amage!2.2 Safety PrecautionsBefo re starting any maintenanc e o r repair pro c ed ures insid e the enc l o sure,take the fo l l o wing measures to prevent o perato r injury o r d amage to the instrument:If nec essary,rinse to xic so l vents fro m the instrument.Avo id inhal ing any vapo rs.Al ways wear gl o ves and go ggl es.If the instrument is c o nnec ted to Chro mel eo n,sel ec t the Disconnect c o mmand in Chro mel eo n to terminate c o mmunic atio n with the d ata system.Turn o ff the instrument.Disc o nnec t the po wer c o rd fro m its so urc e.Warning:Do no t o perate the WPS-3000 Series Nano/Cap Wel l Pl ate Mic ro auto sampl er when the enc l o sure is o pen!So me c o mpo nents insid e the enc l o sure c arry l ine vo l tage.Warning:When wo rking o n the el ec tro nic s,verify that the po wer c o rd is d isc o nnec ted fro m its so urc e.We rec o mmend that the perso ns perfo rming the repair use appro priate gro und ing pro tec tio n to avo id el ec tro static d isc harge,whic h may resul t in d amage to el ec tro nic c o mpo nents.Warning:The c o o l ant used in the WPS-3000 Series Nano/Cap Wel l Pl ate Mic ro auto sampl er is based o n ethyl ene gl yc o l o r pro pyl ene gl yc o l(kno wn as antifreeze).Avo id getting it o n yo ur skin.Warning:Avo id inhal ing any vapo rs.Al ways wear gl o ves and go ggl es.4WPS-3000 Series Well Plate Nan/Cap Microautosampler Service Instructions3 Identifying the Instrument Version3.1 Available ConfigurationsThe WPS-3000 Series Nano/Cap Wel l Pl ate Mic ro auto sampl er is avail abl e in fo ur basic versio ns.Whil e the fo ur versio ns d iffer as d esc ribed bel o w,the user c an c o nfigure a unit to meet the prec ise req uirements o f the appl ic atio ns(e.g.instal l ing d ifferent syringe,need l e o r buffer tubing).Fo r mo re d etail s abo ut the d ifferent c o nfiguratio ns,refer to sec tio ns 8.2-8.4(f pages 35-38).Part No.Description5820.0010WPS-3000(witho ut temperature c o ntro l)inc l ud ing:-2.4 jiL need l e-25|iL syringe-50 jiL buffer tubing-1|1L sampl e l o o p5821.0010WPS-3000B,bio c o mpatibl e versio n5820.0020WPS-3000T,with temperature c o ntro l5821.0020.WPS-300TB,bio c o mpatibl e versio n with temperature c o ntro l3.2 Instrument ModificationsSerial Number(a)Modification(s)Comment2620601El ec tro nic s units eq uipped with a switc hing po wer suppl y.The mo d ified el ec tro