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荧光素激发发射波长.docx

1、荧光素名称激发波长 (nm) 发射波长 (nm) 备注 1,5 IAEDANS 336 490 1,8-ANS 372 480 4-Methylumbelliferone 385 502 5-carboxy-2,7-dichlorofluorescein 504 529 5-Carboxyfluorescein (5-FAM) 492 518 5-Carboxynapthofluorescein (pH 10) 512/598 563/668 Ratio Dye, pH 5-Carboxytetramethylrho

2、damine (5-TAMRA) 542 568 5-FAM (5-Carboxyfluorescein) 492 518 5-HAT (Hydroxy Tryptamine) 370-415 520-540 5-Hydroxy Tryptamine (HAT) 370-415 520-540 5-ROX (carboxy-X-rhodamine) 578 567 604 591 5-TAMRA (5-Carboxytetramethylrhodamine) 548 542 552 568 6-Carboxyrho

3、damine 6G 518 543 6-CR 6G 518 543 6-JOE 520 548 7-Amino-4-methylcoumarin 351 430 7-Aminoactinomycin D (7-AAD) 546 647 7-Hydroxy-4-methylcoumarin 360 449, 455 9-Amino-6-chloro-2-methoxyacridine 412, 430 471, 474 ABQ 344 445 Acid Fuchsin 540 630 AC

4、MA (9-Amino-6-chloro-2-methoxyacridine) 412, 430 471, 474 Acridine Orange + DNA 502 526 Acridine Orange + RNA 460 650 Acridine Orange, both DNA & RNA 440-480 520-650 Acridine Red 455-600 560-680 Acridine Yellow 470 550 Acriflavin 436 520 Acriflavin Feulgen S

5、ITSA 355-425 460 Aequorin (Photoprotein) 466 Photoprotein AFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFP Alexa Fluor 350™ 346 342 442 441 Alexa Fluor 430™ 431 540 Alexa Fluor 488™ 495, 492 519, 520 Alexa Fluor 532™ 531, 532 553

6、 554 Alexa Fluor 546™ 556, 557 572, 573 Alexa Fluor 568™ 577, 578 603 Alexa Fluor 594™ 590, 594 617, 618 Alexa Fluor 633™ 632 650 Alexa Fluor 647™ 647 666 Alexa Fluor 660™ 668 698 Alexa Fluor 680™ 679 702 Alizarin Complexon 530-560, 580 580, 624-645

7、 Alizarin Red 530-560 580 Allophycocyanin (APC) 630, 645 655, 660 AMC, AMCA-S 345 445 AMCA (Aminomethylcoumarin) 345 347 425 444 AMCA-X 353 442 Aminoactinomycin D 555 655 Aminocoumarin 346 350 442 445 Aminomethylcoumarin (AMCA) 345 347 425 444

8、Anilin Blue 600 Anthrocyl stearate 360-381 446 APC (Allophycocyanin) 630, 645 655, 660 APC-Cy7 625-650 755 APTRA-BTC = Ratio Dye, Zn2+ 466/380 520/530 Ratio Dye, Zn2+ APTS 424 505 Astrazon Brilliant Red 4G 500 585 Astrazon Orange R 470 540 Astrazon Red

9、 6B 520 595 Astrazon Yellow 7 GLL 450 480 Atabrine 436 490 ATTO-TAG™ CBQCA 465 560 ATTO-TAG™ FQ 486 591 Auramine 460 550 Aurophosphine G 450 580 Aurophosphine 450-490 515 BAO 9 (Bisaminophenyloxadiazole) 365 395 BCECF (high pH) 492, 503 520,528

10、 BCECF (low pH) 482 520 Berberine Sulphate 430 550 Beta Lactamase 409 447, 520 BFP blue shifted GFP (Y66H) Blue Fluorescent Protein 381, 382, 383 445, 447, 448 blue shifted GFP (Y66H) Blue Fluorescent Protein BFP/GFP FRET Bimane 398 490 Bisbenzamide 360

11、461 Bisbenzimide (Hoechst) 360 461 bis-BTC = Ratio Dye, Zn2+ 455/405 529/505 Ratio Dye, Zn2+ Blancophor FFG 390 470 Blancophor SV 370 435 BOBO™ -1 462 481 BOBO™ -3 570 602 Bodipy 492/515 490 515 Bodipy 493/503 533 549 Bodipy 500/510 509 515 Bod

12、ipy 505/515 502 510 Bodipy 530/550 528 547 Bodipy 542/563 543 563 Bodipy 558/568 558 569 Bodipy 564/570 564 570 Bodipy 576/589 579 590 Bodipy 581/591 584 592 Bodipy 630/650-X 625 642 Bodipy 650/665-X 647 665 Bodipy 665/676 605 676 Bodipy Fl

13、 504, 505 511, 513 Bodipy FL ATP 505 514 Bodipy Fl-Ceramide 505 511 Bodipy R6G SE 528 547 Bodipy TMR 542 574 Bodipy TMR-X conjugate 544 573 Bodipy TMR-X, SE 544 570 Bodipy TR 589 617 Bodipy TR ATP 591 620 Bodipy TR-X SE 588 616 BO-PRO™ -1

14、462 481 BO-PRO™ -3 544 570 Brilliant Sulphoflavin FF 430 520 BTC - Ratio Dye Ca2+ 464/401 533/529 Ratio Dye Ca2+ BTC-5N - atio Dye, Zn2+ 459/417 517/532 Ratio Dye, Zn2+ Calcein 494 517 Calcein Blue 373 440 Calcium Crimson™ 588, 589 611, 615 Calcium Green 5

15、01, 506 531 Calcium Green-1 Ca2+ Dye 506 531 Ca2+ Dye Calcium Green-2 Ca2+ 506/503 536 Ca2+ Calcium Green-5N Ca2+ 506 532 Ca2+ Calcium Green-C18 Ca2+ 509 530 Ca2+ Calcium Orange 549 575 576 Calcofluor White 385, 395, 405 437, 440, 445 Carboxy-X-rhodamine (5-ROX) 57

16、6 601 Cascade Blue™ 377 398 399 420 423 Cascade Yellow 399 400 550 552 Catecholamine 410 470 CCF2 (GeneBlazer) CFDA 494 520 CFP - Cyan Fluorescent Protein 430, 433, 436, (453) 474, 475, 476, (501) Cyan Fluorescent Protein CFP/YFP FRET Chlorop

17、hyll 480 650 Chromomycin A 436-460 470 Chromomycin A 445 575 CL-NERF (Ratio Dye, pH) 504/514 540 Ratio Dye, pH CMFDA 494 520 Coelenterazine Ca2+ Dye, bioluminescence (429) 465 Ca2+ Dye, bioluminescence, native molecule Coelenterazine cp (Ca2+ Dye,) (430) 442 Ca2+

18、Dye, bioluminescence Coelenterazine f (437) 473 Ca2+ Dye, bioluminescence Coelenterazine fcp 452 Ca2+ Dye, bioluminescence Coelenterazine h (437) 464 Ca2+ Dye, bioluminescence Coelenterazine hcp (433) 444 Ca2+ Dye, bioluminescence Coelenterazine ip 441 Ca2+ Dye, bioluminescen

19、ce Coelenterazine n (431) 467 Ca2+ Dye, bioluminescence Coelenterazine O 460 575 Coumarin Phalloidin 387 470 C-phycocyanine CPM Methylcoumarin 384 469 Methylcoumarin CTC 400-450 602 CTC Formazan Cy2™ 489 506 Cy3.1 8 554 568 Cy3.5™ 581 598

20、 Cy3™ 514 552 554 566 570 Cy5.1 8 649 666 Cy5.5™ 675 695 Cy5™ 649 666 670 Cy7™ 710, 743 767, 805 Cyan GFP 433 (453) 475 (501) cyclic AMP Fluorosensor (FiCRhR) 500 517 CyQuant Cell Proliferation Assay 480 520 Cell Proliferation Assay Dabcyl 453

21、 Dansyl 340 578 Dansyl Amine 337 517 Dansyl Cadaverine 335 518 Dansyl Chloride 372 518 Dansyl DHPE 336 517 Dansyl fluoride 356 none DAPI 359 461 Dapoxyl 403 580 Dapoxyl 2 374 574 Dapoxyl 3 373 574 DCFDA 504 529 DCFH (Dichlorodihyd

22、rofluorescein Diacetate) 505 535 DDAO 463 607 DHR (Dihydorhodamine 123) 505 534 Di-4-ANEPPS 496 705 Di-8-ANEPPS (non-ratio) 488 498 605 713 DiA (4-Di-16-ASP) 456 591 Dichlorodihydrofluorescein Diacetate (DCFH) 505 535 DiD - Lipophilic Tracer 644 665 Lip

23、ophilic Tracer DiD (DiIC18(5)) 644 665 DIDS 341 415 Dihydorhodamine 123 (DHR) 505 535 DiI (DiIC18(3)) 549, 551 565 Dinitrophenol 349 DiO (DiOC18(3)) 484, 487 501, 502 DiR 748 780 Lipophilic Tracer DiR (DiIC18(7)) 750 779 DM-NERF (high pH) 497/510 54

24、0 Ratio Dye, pH DNP 349 Dopamine 340 490-520 DsRed 558 583 Red fluorescent protein DTAF 494 520 DY-630-NHS 621 660 Hemicyane label for proteins and DNA DY-635-NHS 634 664 Hemicyane label for proteins and DNA EBFP 383 447 Enhanced Blue Fluorescent Protein ECFP

25、 436 474 Enhanced Cyan Fluorescent Protein EGFP 488, 498 507, 516 Enhanced Green Fluorescent Protein ELF 97 345 530 Eosin 524 545 Erythrosin 529, 532 554, 555 Erythrosin ITC 529 555 Ethidium Bromide 510, 523 595, 605 Ethidium homodimer -1 (EthD-1) 528 617 E

26、uchrysin 430 540 EukoLight Europium (III) chloride EYFP 513, 520 527, 532 Enhanced Yellow Fluorescent Protein Fast Blue 360 440 FDA 494 520 Feulgen (Pararosaniline) 570 625 FIF (Formaldehyd Induced Fluorescence) 405 433 FITC 490, 494 520, 525

27、 FITC Antibody 493 517 Flazo Orange 375-530 612 Fluo-3 480 – 506, 506 520, 527 Fluo-4 494 516 Fluorescein (FITC) 490, 494 520, 525 Fluorescein Diacetate 494 520 Fluoro-Emerald 495 524 Fluoro-Gold (Hydroxystilbamidine) 361 536 Fluor-Ruby 555 582

28、 FluorX 494 520 FM 1-43™ 479 598 FM 4-46 515 640 Fura Red™ (high pH) 572 657 Fura Red™/Fluo-3 Fura-2, high calcium 335 505 Excitation ratio dye Fura-2, low calcium 363 512 Excitation ratio dye Fura-2/BCECF Genacryl Brilliant Red B 520 590 Gen

29、acryl Brilliant Yellow 10GF 430 485 Genacryl Pink 3G 470 583 Genacryl Yellow 5GF 430 475 GeneBlazer (CCF2) GFP (S65T) 498 516 GFP red shifted (rsGFP) 498 516 GFP wild type, non-UV excitation (wtGFP) 475 509 GFP wild type, UV excitation (wtGFP) 395 509

30、 GFPuv 385 508 Gloxalic Acid 405 460 Granular Blue 355 425 Haematoporphyrin 530-560 580 Hoechst 33258 345 487 Hoechst 33342 347 483 Hoechst 34580 392 440 HPTS 355 465 Hydroxycoumarin 325-360 386-455 Hydroxystilbamidine (FluoroGold) 361 536

31、 Hydroxytryptamine 400 530 Indo-1, high calcium 330 401 Emission ratio dye Indo-1, low calcium 346 475 Emission ratio dye Indodicarbocyanine (DiD) 644 665 Indotricarbocyanine (DiR) 748 780 Intrawhite Cf 360 430 JC-1 514 529 JO-JO-1 530 545 JO-PRO-1 532

32、 544 LaserPro 795 812 Laurodan 355 460 LDS 751 (DNA) 543 712 LDS 751 (RNA) 590 607 Leucophor PAF 370 430 Leucophor SF 380 465 Leucophor WS 395 465 Lissamine Rhodamine 572, 577 591, 592 Lissamine Rhodamine B 577 592 LIVE/DEAD Kit Animal Cells

33、 Calcein/Ethidium homodimer 494 528 517 617 for more details refer to LOLO-1 566 580 LO-PRO-1 568 581 Lucifer Yellow 425, 428 528, 536, 540 Lyso Tracker Blue 373 422 Lyso Tracker Blue-White 466 536 Lyso Tracker Green 504, 534 511, 551 Lyso Tracker Red 490

34、 516 Lyso Tracker Yellow 551 576 LysoSensor Blue 374 424 LysoSensor Green 442 505 LysoSensor Yellow/Blue 384 540 Mag Green 507 531 Magdala Red (Phloxin B) 524 600 Mag-Fura Red 483/427 659/631 Ratio Dye, Mg2+ Mag-Fura-2 369/329 369/330 508 511/491 Rat

35、io Dye Ca2+ Ratio Dye Mg2+ Mag-Fura-5 369/330 369/332 505/500 505/482 Ratio Dye, Ca2+ Ratio Dye, Mg2+ Mag-Indo-1 349/328 349/330 480/390 480/417 Ratio Dye, Ca2+ Ratio Dye, Mg2+ Magnesium Green 506, 507 531 Magnesium Orange 550 575 Malachite Green 628 Marina Blue

36、362 459 Maxilon Brilliant Flavin 10 GFF 450 495 Maxilon Brilliant Flavin 8 GFF 460 495 Merocyanin 555 578 Methoxycoumarin 360 410 Mitotracker Green FM 490 516 Mitotracker Orange 551 576 Mitotracker Red 578 599 Mitramycin 450 470 Monobromobimane 3

37、98 490 Monobromobimane (mBBr-GSH) 398 500 Monochlorobimane 380 461 MPS (Methyl Green Pyronine Stilbene) 364 395 NBD 466 539 NBD Amine 450 530 Nile Red 515-555, 559 590, 640 Nitrobenzoxadidole 465 510-650 Noradrenaline 340 490-520 Nuclear Fast Red

38、 289-530 580 Nuclear Yellow 365 495 Nylosan Brilliant Iavin E8G 460 510 Oregon Green 503 522 Oregon Green 488-X 494 517 Oregon Green™ 503 522 Oregon Green™ 488 490, 493 514, 520 Oregon Green™ 500 497 517 Oregon Green™ 514 506 526 Pacific Blue 405

39、 455 Pararosaniline (Feulgen) 570 625 PBFI 340/380 420 Excitation ratio dye PE-Cy5 488 670 PE-Cy7 488 755, 767 PerCP 488 675 PerCP-Cy5.5 488 710 PE-TexasRed [Red 613] 488 613 Phloxin B (Magdala Red) 524 600 Phorwite AR 360 430 Phorwite BKL 37

40、0 430 Phorwite Rev 380 430 Phorwite RPA 375 430 Phosphine 3R 465 565 PhotoResist 365 610 Phycoerythrin B [PE] 546-565 575 Phycoerythrin R [PE] 565 578 PKH26 (Sigma) 551 567 PKH67 496 520 Chroma PMIA 341 376 Pontochrome Blue Black 535-553 605

41、 POPO-1 433 457 POPO-3 533 574 PO-PRO-1 435 455 PO-PRO-3 539 567 Primuline 410 550 Procion Yellow 470 600 Propidium Iodid (PI) (305), 536, 538 617 PyMPO 412, 415 561, 564, 570 Pyrene 360 387 Pyronine 410 540 Pyronine B 540-590 560-650

42、 Pyrozal Brilliant Flavin 7GF 365 495 QSY 7 560 Quinacrine Mustard 440 510 Red 613 [PE-TexasRed] 488 613 Resorufin 571 584, 585 RH 414 532 716 Rhod-2 552 576 Rhodamine 550 573 Rhodamine 110 496, 497 520 Rhodamine 123 507 529 Rhodamine

43、 5 GLD 470 565 Rhodamine 6G 525 555 Rhodamine B 540 625 Rhodamine B 200 523-557 595 Rhodamine B extra 550 605 Rhodamine BB 540 580 Rhodamine BG 540 572 Rhodamine Green 502 527 Rhodamine Phallicidine 558, 542 575, 565 Rhodamine Phalloidine 542 5

44、65 Rhodamine Red 570 590 Rhodamine WT 530 555 Rose Bengal 525, 540 550-600 R-phycocyanine R-phycoerythrin (PE) 565 578 rsGFP 473 509 red shifted GFP (S65T) S65A 471 504 S65C 479 507 S65L 484 510 S65T 488 511 Sapphire GFP 395 511

45、 SBFI 340/380 420 Excitation ratio dye Serotonin 365 520-540 Sevron Brilliant Red 2B 520 595 Sevron Brilliant Red 4G 500 583 Sevron Brilliant Red B 530 590 Sevron Orange 440 530 Sevron Yellow L 430 490 sgBFP™ 387 450 sgBFP™ (super glow BFP) 387 450 Qu

46、antum’s SuperGlo™GFP AFPs sgGFP™ 474 488 sgGFP™ (super glow GFP) 474 509 Quantum’s SuperGlo™GFP AFPs SITS 336 436 Ion Channels SITS (Primuline) 395-425 450 SITS (Stilbene Isothiosulphonic Acid) 365 460 SNAFL calcein 506/535 535/620 Ratio Dye, pH SNAFL-1 508/540 543

47、/623 Ratio Dye, pH SNAFL-2 514/543 546/630 Ratio Dye, pH SNARF calcein 552/574 590/629 Ratio Dye, pH SNARF1 576/548 635/587 Excitation and emission ratio dye Sodium Green 506, 507 532 Na+, K+ SpectrumAqua 433,/53 480/55 Vysis SpectrumGreen 497/30, 509/31 538/44, 524/56 Vysis

48、 SpectrumOrange 559/38, 560 588/48 Vysis Spectrum Red 587, 587/35 612, 612/51 SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium) 344 443 Stilbene 335 440 Sulphorhodamine B can C 520 595 Sulphorhodamine G Extra 470 570 SYTO 11 508, 510 527, 530 Dye for DNA, RNA SYTO 1

49、2 499, 500 522, 519 Dye for DNA, RNA SYTO 13 488, 491 509, 514 Dye for DNA, RNA SYTO 14 517, 521 549, 547 Dye for DNA, RNA SYTO 15 516, 518 546, 555 Dye for DNA, RNA SYTO 16 488, 494 518, 525 Dye for DNA, RNA SYTO 17 621 634 Dye for DNA SYTO 18 490, 493 507, 527 Dye for DNA, RNA SYTO 20 512 530 Dye for DNA SYTO 21 494 517 Dye for DNA SYTO 22 515 535 Dye for DNA SYTO 23 499 520 Dye for DNA SYTO 24 490 515 Dye for DNA SYTO 25 521 556 Dye for DNA SYTO 40 420 441 Dye for DNA SYTO 41 430 454 Dye for DNA SYTO 42 433 460 Dye

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