荧光素激发发射波长.docx

荧光素名称
激发波长 (nm)
发射波长 (nm)
备注
1,5 IAEDANS
336
490

1,8-ANS
372
480

4-Methylumbelliferone
385
502

5-carboxy-2,7-dichlorofluorescein
504
529

5-Carboxyfluorescein (5-FAM)
492
518

5-Carboxynapthofluorescein (pH 10)
512/598
563/668
Ratio Dye, pH
5-Carboxytetramethylrhodamine (5-TAMRA)
542
568

5-FAM (5-Carboxyfluorescein)
492
518

5-HAT (Hydroxy Tryptamine)
370-415
520-540

5-Hydroxy Tryptamine (HAT)
370-415
520-540

5-ROX (carboxy-X-rhodamine)
578
567
604
591

5-TAMRA (5-Carboxytetramethylrhodamine)
548
542
552
568

6-Carboxyrhodamine 6G
518
543

6-CR 6G
518
543

6-JOE
520
548

7-Amino-4-methylcoumarin
351
430

7-Aminoactinomycin D (7-AAD)
546
647

7-Hydroxy-4-methylcoumarin
360
449, 455

9-Amino-6-chloro-2-methoxyacridine
412, 430
471, 474

ABQ
344
445

Acid Fuchsin
540
630

ACMA (9-Amino-6-chloro-2-methoxyacridine)
412, 430
471, 474

Acridine Orange + DNA
502
526

Acridine Orange + RNA
460
650

Acridine Orange, both DNA & RNA
440-480
520-650

Acridine Red
455-600
560-680

Acridine Yellow
470
550

Acriflavin
436
520

Acriflavin Feulgen SITSA
355-425
460

Aequorin (Photoprotein)

466
Photoprotein
AFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFP

Alexa Fluor 350™
346
342
442
441

Alexa Fluor 430™
431
540

Alexa Fluor 488™
495, 492
519, 520

Alexa Fluor 532™
531, 532
553, 554

Alexa Fluor 546™
556, 557
572, 573

Alexa Fluor 568™
577, 578
603

Alexa Fluor 594™
590, 594
617, 618

Alexa Fluor 633™
632
650

Alexa Fluor 647™
647
666

Alexa Fluor 660™
668
698

Alexa Fluor 680™
679
702

Alizarin Complexon
530-560, 580
580, 624-645

Alizarin Red
530-560
580

Allophycocyanin (APC)
630, 645
655, 660

AMC, AMCA-S
345
445

AMCA (Aminomethylcoumarin)
345
347
425
444

AMCA-X
353
442

Aminoactinomycin D
555
655

Aminocoumarin
346
350
442
445

Aminomethylcoumarin (AMCA)
345
347
425
444

Anilin Blue

600

Anthrocyl stearate
360-381
446

APC (Allophycocyanin)
630, 645
655, 660

APC-Cy7
625-650
755

APTRA-BTC = Ratio Dye, Zn2+
466/380
520/530
Ratio Dye, Zn2+
APTS
424
505

Astrazon Brilliant Red 4G
500
585

Astrazon Orange R
470
540

Astrazon Red 6B
520
595

Astrazon Yellow 7 GLL
450
480

Atabrine
436
490

ATTO-TAG™ CBQCA
465
560

ATTO-TAG™ FQ
486
591

Auramine
460
550

Aurophosphine G
450
580

Aurophosphine
450-490
515

BAO 9 (Bisaminophenyloxadiazole)
365
395

BCECF (high pH)
492, 503
520,528

BCECF (low pH)
482
520

Berberine Sulphate
430
550

Beta Lactamase
409
447, 520

BFP blue shifted GFP (Y66H)
Blue Fluorescent Protein
381, 382, 383
445, 447, 448
blue shifted GFP (Y66H)
Blue Fluorescent Protein
BFP/GFP FRET

Bimane
398
490

Bisbenzamide
360
461

Bisbenzimide (Hoechst)
360
461

bis-BTC = Ratio Dye, Zn2+
455/405
529/505
Ratio Dye, Zn2+
Blancophor FFG
390
470

Blancophor SV
370
435

BOBO™ -1
462
481

BOBO™ -3
570
602

Bodipy 492/515
490
515

Bodipy 493/503
533
549

Bodipy 500/510
509
515

Bodipy 505/515
502
510

Bodipy 530/550
528
547

Bodipy 542/563
543
563

Bodipy 558/568
558
569

Bodipy 564/570
564
570

Bodipy 576/589
579
590

Bodipy 581/591
584
592

Bodipy 630/650-X
625
642

Bodipy 650/665-X
647
665

Bodipy 665/676
605
676

Bodipy Fl
504, 505
511, 513

Bodipy FL ATP
505
514

Bodipy Fl-Ceramide
505
511

Bodipy R6G SE
528
547

Bodipy TMR
542
574

Bodipy TMR-X conjugate
544
573

Bodipy TMR-X, SE
544
570

Bodipy TR
589
617

Bodipy TR ATP
591
620

Bodipy TR-X SE
588
616

BO-PRO™ -1
462
481

BO-PRO™ -3
544
570

Brilliant Sulphoflavin FF
430
520

BTC - Ratio Dye Ca2+
464/401
533/529
Ratio Dye Ca2+
BTC-5N - atio Dye, Zn2+
459/417
517/532
Ratio Dye, Zn2+
Calcein
494
517

Calcein Blue
373
440

Calcium Crimson™
588, 589
611, 615

Calcium Green
501, 506
531

Calcium Green-1 Ca2+ Dye
506
531
Ca2+ Dye
Calcium Green-2 Ca2+
506/503
536
Ca2+
Calcium Green-5N Ca2+
506
532
Ca2+
Calcium Green-C18 Ca2+
509
530
Ca2+
Calcium Orange
549
575
576

Calcofluor White
385, 395, 405
437, 440, 445

Carboxy-X-rhodamine (5-ROX)
576
601

Cascade Blue™
377
398
399
420
423

Cascade Yellow
399
400
550
552

Catecholamine
410
470

CCF2 (GeneBlazer)

CFDA
494
520

CFP - Cyan Fluorescent Protein
430, 433, 436, (453)
474, 475, 476, (501)
Cyan Fluorescent Protein
CFP/YFP FRET

Chlorophyll
480
650

Chromomycin A
436-460
470

Chromomycin A
445
575

CL-NERF (Ratio Dye, pH)
504/514
540
Ratio Dye, pH
CMFDA
494
520

Coelenterazine Ca2+ Dye, bioluminescence
(429)
465
Ca2+ Dye, bioluminescence,
native molecule
Coelenterazine cp (Ca2+ Dye,)
(430)
442
Ca2+ Dye, bioluminescence
Coelenterazine f
(437)
473
Ca2+ Dye, bioluminescence
Coelenterazine fcp

452
Ca2+ Dye, bioluminescence
Coelenterazine h
(437)
464
Ca2+ Dye, bioluminescence
Coelenterazine hcp
(433)
444
Ca2+ Dye, bioluminescence
Coelenterazine ip

441
Ca2+ Dye, bioluminescence
Coelenterazine n
(431)
467
Ca2+ Dye, bioluminescence
Coelenterazine O
460
575

Coumarin Phalloidin
387
470

C-phycocyanine

CPM Methylcoumarin
384
469
Methylcoumarin
CTC
400-450
602

CTC Formazan

Cy2™
489
506

Cy3.1 8
554
568

Cy3.5™
581
598

Cy3™
514
552
554
566
570

Cy5.1 8
649
666

Cy5.5™
675
695

Cy5™
649
666
670

Cy7™
710, 743
767, 805

Cyan GFP
433 (453)
475 (501)

cyclic AMP Fluorosensor (FiCRhR)
500
517

CyQuant Cell Proliferation Assay
480
520
Cell Proliferation Assay
Dabcyl
453

Dansyl
340
578

Dansyl Amine
337
517

Dansyl Cadaverine
335
518

Dansyl Chloride
372
518

Dansyl DHPE
336
517

Dansyl fluoride
356
none

DAPI
359
461

Dapoxyl
403
580

Dapoxyl 2
374
574

Dapoxyl 3
373
574

DCFDA
504
529

DCFH (Dichlorodihydrofluorescein Diacetate)
505
535

DDAO
463
607

DHR (Dihydorhodamine 123)
505
534

Di-4-ANEPPS
496
705

Di-8-ANEPPS (non-ratio)
488
498
605
713

DiA (4-Di-16-ASP)
456
591

Dichlorodihydrofluorescein Diacetate (DCFH)
505
535

DiD - Lipophilic Tracer
644
665
Lipophilic Tracer
DiD (DiIC18(5))
644
665

DIDS
341
415

Dihydorhodamine 123 (DHR)
505
535

DiI (DiIC18(3))
549, 551
565

Dinitrophenol
349

DiO (DiOC18(3))
484, 487
501, 502

DiR
748
780
Lipophilic Tracer
DiR (DiIC18(7))
750
779

DM-NERF (high pH)
497/510
540
Ratio Dye, pH
DNP
349

Dopamine
340
490-520

DsRed
558
583
Red fluorescent protein
DTAF
494
520

DY-630-NHS
621
660
Hemicyane
label for proteins and DNA
DY-635-NHS
634
664
Hemicyane
label for proteins and DNA
EBFP
383
447
Enhanced Blue Fluorescent Protein
ECFP
436
474
Enhanced Cyan Fluorescent Protein
EGFP
488, 498
507, 516
Enhanced Green Fluorescent Protein
ELF 97
345
530

Eosin
524
545

Erythrosin
529, 532
554, 555

Erythrosin ITC
529
555

Ethidium Bromide
510, 523
595, 605

Ethidium homodimer -1 (EthD-1)
528
617

Euchrysin
430
540

EukoLight

Europium (III) chloride

EYFP
513, 520
527, 532
Enhanced Yellow Fluorescent Protein
Fast Blue
360
440

FDA
494
520

Feulgen (Pararosaniline)
570
625

FIF (Formaldehyd Induced Fluorescence)
405
433

FITC
490, 494
520, 525

FITC Antibody
493
517

Flazo Orange
375-530
612

Fluo-3
480 – 506, 506
520, 527

Fluo-4
494
516

Fluorescein (FITC)
490, 494
520, 525

Fluorescein Diacetate
494
520

Fluoro-Emerald
495
524

Fluoro-Gold (Hydroxystilbamidine)
361
536

Fluor-Ruby
555
582

FluorX
494
520

FM 1-43™
479
598

FM 4-46
515
640

Fura Red™ (high pH)
572
657

Fura Red™/Fluo-3

Fura-2, high calcium
335
505
Excitation ratio dye
Fura-2, low calcium
363
512
Excitation ratio dye
Fura-2/BCECF

Genacryl Brilliant Red B
520
590

Genacryl Brilliant Yellow 10GF
430
485

Genacryl Pink 3G
470
583

Genacryl Yellow 5GF
430
475

GeneBlazer (CCF2)

GFP (S65T)
498
516

GFP red shifted (rsGFP)
498
516

GFP wild type, non-UV excitation (wtGFP)
475
509

GFP wild type, UV excitation (wtGFP)
395
509

GFPuv
385
508

Gloxalic Acid
405
460

Granular Blue
355
425

Haematoporphyrin
530-560
580

Hoechst 33258
345
487

Hoechst 33342
347
483

Hoechst 34580
392
440

HPTS
355
465

Hydroxycoumarin
325-360
386-455

Hydroxystilbamidine (FluoroGold)
361
536

Hydroxytryptamine
400
530

Indo-1, high calcium
330
401
Emission ratio dye
Indo-1, low calcium
346
475
Emission ratio dye
Indodicarbocyanine (DiD)
644
665

Indotricarbocyanine (DiR)
748
780

Intrawhite Cf
360
430

JC-1
514
529

JO-JO-1
530
545

JO-PRO-1
532
544

LaserPro
795
812

Laurodan
355
460

LDS 751 (DNA)
543
712

LDS 751 (RNA)
590
607

Leucophor PAF
370
430

Leucophor SF
380
465

Leucophor WS
395
465

Lissamine Rhodamine
572, 577
591, 592

Lissamine Rhodamine B
577
592

LIVE/DEAD Kit Animal Cells
Calcein/Ethidium homodimer
494
528
517
617
for more details refer
to 
LOLO-1
566
580

LO-PRO-1
568
581

Lucifer Yellow
425, 428
528, 536, 540

Lyso Tracker Blue
373
422

Lyso Tracker Blue-White
466
536

Lyso Tracker Green
504, 534
511, 551

Lyso Tracker Red
490
516

Lyso Tracker Yellow
551
576

LysoSensor Blue
374
424

LysoSensor Green
442
505

LysoSensor Yellow/Blue
384
540

Mag Green
507
531

Magdala Red (Phloxin B)
524
600

Mag-Fura Red
483/427
659/631
Ratio Dye, Mg2+
Mag-Fura-2
369/329
369/330
508
511/491
Ratio Dye Ca2+
Ratio Dye Mg2+
Mag-Fura-5
369/330
369/332
505/500
505/482
Ratio Dye, Ca2+
Ratio Dye, Mg2+
Mag-Indo-1
349/328
349/330
480/390
480/417
Ratio Dye, Ca2+
Ratio Dye, Mg2+
Magnesium Green
506, 507
531

Magnesium Orange
550
575

Malachite Green
628

Marina Blue
362
459

Maxilon Brilliant Flavin 10 GFF
450
495

Maxilon Brilliant Flavin 8 GFF
460
495

Merocyanin
555
578

Methoxycoumarin
360
410

Mitotracker Green FM
490
516

Mitotracker Orange
551
576

Mitotracker Red
578
599

Mitramycin
450
470

Monobromobimane
398
490

Monobromobimane (mBBr-GSH)
398
500

Monochlorobimane
380
461

MPS (Methyl Green Pyronine Stilbene)
364
395

NBD
466
539

NBD Amine
450
530

Nile Red
515-555, 559
590, 640

Nitrobenzoxadidole
465
510-650

Noradrenaline
340
490-520

Nuclear Fast Red
289-530
580

Nuclear Yellow
365
495

Nylosan Brilliant Iavin E8G
460
510

Oregon Green
503
522

Oregon Green 488-X
494
517

Oregon Green™
503
522

Oregon Green™ 488
490, 493
514, 520

Oregon Green™ 500
497
517

Oregon Green™ 514
506
526

Pacific Blue
405
455

Pararosaniline (Feulgen)
570
625

PBFI
340/380
420
Excitation ratio dye
PE-Cy5
488
670

PE-Cy7
488
755, 767

PerCP
488
675

PerCP-Cy5.5
488
710

PE-TexasRed [Red 613]
488
613

Phloxin B (Magdala Red)
524
600

Phorwite AR
360
430

Phorwite BKL
370
430

Phorwite Rev
380
430

Phorwite RPA
375
430

Phosphine 3R
465
565

PhotoResist
365
610

Phycoerythrin B [PE]
546-565
575

Phycoerythrin R [PE]
565
578

PKH26 (Sigma)
551
567

PKH67
496
520
Chroma
PMIA
341
376

Pontochrome Blue Black
535-553
605

POPO-1
433
457

POPO-3
533
574

PO-PRO-1
435
455

PO-PRO-3
539
567

Primuline
410
550

Procion Yellow
470
600

Propidium Iodid (PI)
(305), 536, 538
617

PyMPO
412, 415
561, 564, 570

Pyrene
360
387

Pyronine
410
540

Pyronine B
540-590
560-650

Pyrozal Brilliant Flavin 7GF
365
495

QSY 7
560

Quinacrine Mustard
440
510

Red 613 [PE-TexasRed]
488
613

Resorufin
571
584, 585

RH 414
532
716

Rhod-2
552
576

Rhodamine
550
573

Rhodamine 110
496, 497
520

Rhodamine 123
507
529

Rhodamine 5 GLD
470
565

Rhodamine 6G
525
555

Rhodamine B
540
625

Rhodamine B 200
523-557
595

Rhodamine B extra
550
605

Rhodamine BB
540
580

Rhodamine BG
540
572

Rhodamine Green
502
527

Rhodamine Phallicidine
558, 542
575, 565

Rhodamine Phalloidine
542
565

Rhodamine Red
570
590

Rhodamine WT
530
555

Rose Bengal
525, 540
550-600

R-phycocyanine

R-phycoerythrin (PE)
565
578

rsGFP
473
509
red shifted GFP (S65T)
S65A
471
504

S65C
479
507

S65L
484
510

S65T
488
511

Sapphire GFP
395
511

SBFI
340/380
420
Excitation ratio dye
Serotonin
365
520-540

Sevron Brilliant Red 2B
520
595

Sevron Brilliant Red 4G
500
583

Sevron Brilliant Red B
530
590

Sevron Orange
440
530

Sevron Yellow L
430
490

sgBFP™
387
450

sgBFP™ (super glow BFP)
387
450
Quantum’s SuperGlo™GFP
AFPs
sgGFP™
474
488

sgGFP™ (super glow GFP)
474
509
Quantum’s SuperGlo™GFP
AFPs
SITS
336
436
Ion Channels
SITS (Primuline)
395-425
450

SITS (Stilbene Isothiosulphonic Acid)
365
460

SNAFL calcein
506/535
535/620
Ratio Dye, pH
SNAFL-1
508/540
543/623
Ratio Dye, pH
SNAFL-2
514/543
546/630
Ratio Dye, pH
SNARF calcein
552/574
590/629
Ratio Dye, pH
SNARF1
576/548
635/587
Excitation and emission ratio dye
Sodium Green
506, 507
532
Na+, K+
SpectrumAqua
433,/53
480/55
Vysis
SpectrumGreen
497/30, 509/31
538/44, 524/56
Vysis
SpectrumOrange
559/38, 560
588/48
Vysis
Spectrum Red
587, 587/35
612, 612/51

SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium)
344
443

Stilbene
335
440

Sulphorhodamine B can C
520
595

Sulphorhodamine G Extra
470
570

SYTO 11
508, 510
527, 530
Dye for DNA, RNA
SYTO 12
499, 500
522, 519
Dye for DNA, RNA
SYTO 13
488, 491
509, 514
Dye for DNA, RNA
SYTO 14
517, 521
549, 547
Dye for DNA, RNA
SYTO 15
516, 518
546, 555
Dye for DNA, RNA
SYTO 16
488, 494
518, 525
Dye for DNA, RNA
SYTO 17
621
634
Dye for DNA
SYTO 18
490, 493
507, 527
Dye for DNA, RNA
SYTO 20
512
530
Dye for DNA
SYTO 21
494
517
Dye for DNA
SYTO 22
515
535
Dye for DNA
SYTO 23
499
520
Dye for DNA
SYTO 24
490
515
Dye for DNA
SYTO 25
521
556
Dye for DNA
SYTO 40
420
441
Dye for DNA
SYTO 41
430
454
Dye for DNA
SYTO 42
433
460
Dye