RNA建库流程PPT课件.ppt

RNA建库流程葛玮珍2014.4.11.From Weihua Zeng&Ali Mortazavi Nature immunology一、RNA-seq实验流程样品制备样品检测文库构建文库库检上机测序1.二、文库类型RNAmRNADGE文库真核普通转录组文库真核链特异性文库原核链特异性文库Non-coding RNAlncRNA文库Small RNA文库根据研究对象不同选择相应的建库方法：.pmRNA/lncRNA建库流程.Oligo dT磁珠富集mRNARibo-zero kit去除rRNA4.产品厂家货号规格Ribo-Zero Magnetic Kit（bacteria）EpicentreMRZB124246/24RxnsRibo-Zero Magnetic Kit(Plant Leaf)EpicentreMRZPL1166/24RxnsRibo-Zero Magnetic Kit(Plant Seed/Root)EpicentreMRZSR1166/24RxnsRibo-Zero Magnetic Gold Kit(Epidemiology)EpicentreMRZE7066/24RxnsRibo-Zero Magnetic Gold Kit(Human/Mouse/Rat)EpicentreMRZG123246/24RxnsRibo-zero kit去除rRNA5.Ribo-Zero Kit Selection Guidehttp:/ RNA文库构建流程POH.如何避免降解的mRNA/rRNA等污染：样品严格质检，RIN值合格。PAGE胶电泳纯化，回收符合small RNA大小的片段（140-160bp）。M1 1 1 1 M2160bp140bp.Q&A?10.