荧光物质波长资料(表格).doc

荧光物质波长对照表
荧光物质
激发光波长Ex(nm)
发射光波长Em(nm)
Acid Fuchsin
540
630
Acridine Orange(Bound to DNA)
502
526
Acridine Red
455-600
560-680
Acridine Yellow
470
550
Acriflavin
436
520
AFA (AcriflavinFeulgen SITSA)
355-425
460
Alizarin Complexon
530-560
580
Alizarin Red
530-560
580
Allophycocyanin
650
661
ACMA
430
474
AMCA-S, AMC
345
445
Aminoactinomycin D
555
655
7-Aminoactinomycin D-AAD
546
647
Aminocoumarin
350
445
Anthroyl Stearate
361-381
446
Astrazon BrilliantRed 4G
500
585
Astrazon Orange R
470
540
Astrazon Red 6B
520
595
Astrazon Yellow7 GLL
450
480
Atabrine
436
490
Auramine
460
550
Aurophosphine
450-490
515
Aurophosphine G
450
580
BAO 9-(Bisamino-phenyloxadiazole)
365
395
BCECF
505
530
Berberine Sulphate
430
550
Bisbenzamide
360
600-610
BOBO-1, BO-PRO-1
462
481
Blancophor FFG Solution
390
470
Blancophor SV
370
435
Bodipy Fl
503
512
Bodipy TMR
542
574
Bodipy TR
589
617
BOPRO 1
462
481
Brilliant Sulpho-flavin FF
430
520
Calcein
494
517
Calcien Blue
370
435
Calcium Green
505
532
Calcium Orange
549
576
Calcofluor RW Solution
370
440
Calcofluor White
440
500-520
Calcofluor WhiteABT Solution
380
475
Calcofluor WhiteStandard Solution
365
435
5-(and 6-)carboxySNARF-1 indicator 548(low pH)576(high pH) 587(low pH)635(high pH)
6-Carboxyrhodamine 6G
525
555
Cascade Blue
400
425
Catecholamine
410
470
Chinacrine
450-490
515
CL-NERF 504(low pH)514(high pH) 587(low pH)540(high pH)
Coriphosphine O
460
575
Coumarin-Phalloidin
387
470
CY3.18
554
568
CY5.18
649
666
CY7
710
805
DANS (1-DimethylAmino-Naphthaline-5-Sulphonic Acid)
340
525
DANSA(DiaminoNaphthyl-Sulphonic Acid)
340-380
430
Dansyl NH-CH3 in water
340
578
DAPI
350
470
DiA
456
590
Diamino PhenylOxydiazole (DAO)
280
460
Di-8-ANEPPS
488
605
Dimethylamino-5-Sulphonic Acid
310-370
520
DiI [DiIC18(3)]
549
565
DiO [DiOC18(3)]
484
501
Diphenyl BrilliantFlavine 7GFF
430
520
DM-NERF 497(low pH)510(high pH) 527(low pH)536(high pH) Dopamine
340
490-520
ELF-97 alcohol
345
530
Eosin
525
545
Erythrosin ITC
530
558
Ethidium Bromide
510
595
Euchrysin
430
540
FIF (FormaldehydeInduced Fluorescence)
405
435
Flazo Orange
375-530
612
Fluorescein
494
518
Fluorescein Iso-thiocyanate (FITC)
490
525
Fluo 3
485
503
FM1-43
479
598
Fura-2 363(low [Ca2+])335(high [Ca2+]) 512(low [Ca2+])505(high [Ca2+])
Fura Red 472(low [Ca2+])436(high [Ca2+]) 657(low [Ca2+])637(high [Ca2+])
Genacryl BrilliantRed B
520
590
Genacryl BrilliantYellow 10GF
430
485
Genacryl Pink 3G
470
583
Genacryl Yellow5GF
430
475
Gloxalic Acid
405
460
Granular Blue
355
425
Haematoporphyrin
530-560
580
Hoechst 33258, 33342(Bound to DNA)
352
461
3-Hydroxypyrene-5,-8,10-TriSulfonic Acid
403
513
7-Hydroxy-4-methylcoumarin
360
455
5-Hydroxy-Tryptamine (5-HT)
380-415
520-530
Indo-1
350
405-482
Intrawhite CfLiquid
360
430
Leucophor PAF
370
430
Leucophor SF
380
465
Leucophor WS
395
465
Lissamine RhodamineB200 (RD200)
575
595
Lucifer Yellow CH
425
528
Lucifer Yellow VS
430
535
LysoSensor BlueDND-192, DND-167
374
425
LysoSensor GreenDND-153, DND-189
442
505
LysoSensor Yellow/Blue 384(low pH)329(high pH) 540(low pH)440(high pH)
LysoTracker Green
504
511
LysoTracker Yellow
534
551
LysoTracker Red
577
592
Magdala Red
524
600
Magnesium Green
506
531
Magnesium Orange
550
575
Maxilon BrilliantFlavin 10 GFF
450
495
Maxilon BrilliantFlavin 8 GFF
460
495
Mitotracker Green FM
490
516
Mitotracker OrangeCMTMRos
551
576
MPS (Methyl GreenPyronine Stilbene)
364
395
Mithramycin
450
570
NBD
465
535
NBD Amine
450
530
Nile Red
515-530
525-605
Nitrobenzoxadidole
460-470
510-650
Noradrenaline
340
490-520
Nuclear Fast Red
289-530
580
Nuclear Yellow
365
495
Nylosan BrilliantFlavin E8G
460
510
Oregon Green 488fluorophore
496
524
Oregon Green 500fluorophore
503
522
Oregon Green 514fluorophore
511
530
Pararosaniline(Feulgen)
570
625
Phorwite ARSolution
360
430
Phorwite BKL
370
430
Phorwite Rev
380
430
Phorwite RPA
375
430
Phosphine 3R
465
565
Phosphine R
480-565
578
PontochromeBlue Black
535-553
605
POPO-1, PO-PRO-1
434
456
Primuline
410
550
Procion Yellow
470
600
Propidium Iodide
536
617
Pyronine
410
540
Pyronine B
540-590
560-650
Pyrozal BrilliantFlavin 7GF
365
495
QuinacrineMustard
423
503
R-phycoerythrin
565
575
Rhodamine 110
496
520
Rhodamine 123
511
534
Rhodamine 5 GLD
470
565
Rhodamine 6G
526
555
Rhodamine B
540
625
Rhodamine B 200
523-557
595
Rhodamine B Extra
550
605
Rhodamine BB
540
580
Rhodamine BG
540
572
Rhodamine Greenfluorophore
502
527
Rhodamine Red
570
590
Rhodamine WT
530
555
Rhodol Green fluorophore
499
525
Rose Bengal
540
550-600
Serotonin
365
520-540
Sevron BrilliantRed 2B
520
595
Sevron BrilliantRed 4G
500
583
Sevron BrilliantRed B
530
590
Sevron Orange
440
530
Sevron Yellow L
430
490
SITS (Primuline)
395-425
450
SITS (StilbeneIsothiosulphonic Acid)
365
460
Sodium Green
507
535
Stilbene
335
440
Snarf 1
563
639
Sulpho RhodamineB Can C
520
595
Sulpho RhodamineG Extra
470
570
SYTOX Green nucleicacid stain
504
523
SYTO Green fluorescentnucleic acid stains
494
515
SYTO Green fluorescentnucleic acid stains
515
543 ?13
SYTO 17 red fluorescentnucleic acid stain
621
634
Tetracycline
390
560
TRITC (TetramethylRhodamine Isothiocyanate)
557
576
Texas Red
596
615
Thiazine Red R
510
580
Thioflavin S
430
550
Thioflavin TCN
350
460
Thioflavin 5
430
550
Thiolyte
370-385
477-484
Thiozol Orange
453
480
Tinopol CBS
390
430
TOTO 1, TO-RRO-1
514
533
TOTO 3, TO-PRO-3
642
661
True Blue
365
420-430
Ultralite
656
678
Uranine B
420
520
Uvitex SFC
365
435
X-Rhodamine
580
605
Xylene Orange
546
580
XRITC
582
601
YOYO-1, YOYO-PRO-1
491
509
YOYO-3, YOYO-PRO-3
612
631