常用蛋白酶酶切位点.docx

Cleavage Sites Table for Fusion Proteins：
PROTEASE
COMPANY
Protease Capture
Thrombin
Leu-Val-Pro-Arg��Gly-Ser
Amersham-Biosciences, Novagen,
 SIGMA, Roche
Benzamidine-Agarose
Factor Xa 
Ile-Glu/Asp-Gly-Arg�� 
Amersham-Biosciences, New England Biolabs, 
Roche
Benzamidine-Agarose
Enterokinase
Asp-Asp-Asp-Asp-Lys��
New England Biolabs,
 Novagen, Roche
Trypsin Inhibitor-Agarose
TEV protease
Glu-Asn-Leu-Tyr-Phe-Gln��Gly
Invitrogen – Life Technologies
 Ni-NTA (6His recomb. TEV)
PreScission
Leu-Glu-Val-Leu-Phe-Gln��Gly-Pro
Amersham-Biosciences
GSTrap for GST fusion enzyme
TAGZyme 
His-tag removal by Exoproteolytic Digestion
Qiagen
Ni-NTA (6His recomb. enzyme)
Intein Site 
dithiothreitol cleavage
New England Biolabs
DTT elimination by dialysis 
HRV 3C Protease  
Leu-Glu-Val-Leu-Phe-Gln��Gly-Pro
Novagen    
Ni-NTA (6His recomb. enzyme)
SUMO Protease
recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO 
Invitrogen – Life Technologies, LifeSensors
Ni-NTA (6His recomb. enzyme)