人肌红蛋白基因外显子一的生物信息学分析.doc

人肌红蛋白基因外显子一的生物信息学分析
一． Introduction：Myoglobin, the article is composed of a peptide chains and a binding protein of heme prosthetic group, is within the muscle oxygen storage protein, its oxygen saturation of the elliptic curve.Myoglobin is the main protein of skeletal muscle and cardiac muscle, when muscle damage, leakage to the circulating in the blood in muscle tissue, increase the serum concentration of myoglobin, the index is used to determine whether muscle damage.
二． Purpose：对人肌红蛋白基因外显子一基因序列使用 “Bioedit和DNAMAN、anthe5.0”及相关网站进行GC含量统计、电子酶切、引物设计、基因在人类基因组中的定位、电子克隆及蛋白在细胞内的定位、氨基酸组成统计、分子量、等电点、亲疏水性、信号肽分析（跨膜区域）、二级结构（含保守结构域）及三维结构分析，miR分析，启动子分析等。其中抗原表位分析、多序列比对和进化分析等。
三． The nature and function of myoglobin：
Nature: myoglobin = a polypeptide chain + a prosthetic group polypeptide chain, composed of 153 amino acid residues
Prosthetic group: heme prosthetic group
Molecular weight: 16, 700
Function: transport of oxygen and oxygen storage function in muscle tertiary structure of myoglobin
四． Key word: 肌红蛋白（myoglobin）
五． Implements and methods：
采用Bioedit和DNAMAN分析软件工具和在线有关蛋白质和核酸方面的分析软件，分别进行核酸和蛋白质两个方面的分析。
核酸分析首先从pubmed核酸数据库中搜索到人的Myoglobin核酸序列，分别进行序列的基本性质分析，限制性酶谱分析及其基因在染色体上的定位分析。具体操作方法将根据上述各个软件上“Help”文件进行，基因在染色体上的定位分析根据网页上的操作说明进行。
从pubmed蛋白质数据库中搜索到人的Myoglobin和整个前体Myoglobin蛋白质序列，分别进行蛋白序列的基本性质分析（氨基酸组成、分子量、等电点），亲疏水性分析，蛋白酶切位点分析，信号肽序列分析，二级结构和三维结构分析，同源序列和进化分析。二级结构分析包括结构功能域、Motif、保守位点等，具体操作方法将根据上述各个软件上“Help”文件和网页上的操作说明进行。
Resluts:
1.从NCBI中可以得到一下有关Myoglobi蛋白分子的信息：
Human myoglobin gene, exon 1
GenBank: M10090.1

LOCUS HUMMGI1 2552 bp DNA linear PRI 07-JAN-1995
DEFINITION Human myoglobin gene, exon 1.
ACCESSION M10090
VERSION M10090.1 GI:187580
KEYWORDS Fok family repetitive sequence; direct repeat; myoglobin; repeat
region; tandem repeat.
SEGMENT 1 of 3
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2552)
AUTHORS Akaboshi,E.
TITLE Cloning of the human myoglobin gene
JOURNAL Gene 33 (3), 241-249 (1985)
PUBMED 2989088
COMMENT Original source text: Human: fetal liver DNA (library of Lawn et
al.), clone lambda-HMB151; delta-thalassemia leukocyte DNA (library
of Kimura et al.), clone lambda-HMB159.
Draft entry and computer-readable copy of sequence [1] kindly
provided by E.Akaboshi, 22-OCT-1985.
A tandem repeat sequence is found at position 142-794. Position of
the mRNA cap site was deduced from that of the seal myoglobin gene
(Blanchetot et al. 1983).
FEATURES Location/Qualifiers
source 1..2552
/organism="Homo sapiens"
/mol_type="genomic DNA"
/db_xref="taxon:9606"
/map="22q11.2-qter"
repeat_region 1100..1750
/note="Fok family repetitive element"
prim_transcript 1895..>2552
/note="myoglobin mRNA and intron"
gene join(1965..2552,M14602.1:1..2671,M14603.1:1..501)
/gene="MB"
CDS join(1965..2059,M14602.1:1415..1637,M14603.1:355..501)
/gene="MB"
/note="myoglobin"
/codon_start=1
/protein_id="AAA59595.1"
/db_xref="GI:386872"
/translation="MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKF
DKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPV
KYLEFISECIIQVLQSKHPGDFGADAEGAMNKALELFRKDMASNYKELGFQG"
exon <1965..2059
/gene="MB"
/note="myoglobin; G00-119-378"
/number=1
intron 2060..>2552
/gene="MB"
/note="myoglobin intron A"
ORIGIN 2 bp upstream of RsaI site.
1 gtactgtatt ttcattcctc ttagttatct ccctaaaaag actctgagtt ccttgaacac
61 aggaaggtgt tttatttgat tttgttatcc tcagcatgta gcagtgtctg acacacagta
121 ggtgctctat cactgtgaga gggatggatg gatgggtgga gttacagatg gatagaagga
181 tagatggagg gatgggtgga tgatggatgg atagatggat ggagggggga tgatgaatgg
241 agggataatg agtggatgaa tgagggaatg ggtggatgga tggatggagg gatggaggaa
301 cagatagata gatggaggga tgggtgggtg atggatggat agatggatgg agggagggat
361 gatgaatgga gggataatga atggatgaat gaggggatgg gtggatggat gaatggaggg
421 atgatgggtg gatgaatgaa ttgagggatg gatggatgaa cacatggatg gatggataga
481 tggatagatg gaggaactgg tggattttgg atggatgggt ggatggatag atgaatgaat
541 gcctggatag acaaagagat gatggataga tgaatagatg aattaaggga tgtcggatag
601 atggagggat tgatagatgt tggatggatg ggtggtggat ggatagatga gtgaatgcat
661 ggatagacaa agagatgatg gatggatgaa ttaagggatg acagatggat ggatggatga
721 gtaactggat ggacaagtgg ataaatggat agatggttga atacctgaat ggattgaagg
781 aggatgcatg gatgtaagat aaggctaatc atcctccact ctctttcttt gcaaaaccat
841 ccacccattt actcaataaa catttattca gttcaaactt ggcacaaagc accatgtgag
901 gcccaagaga tacgtgggtt aataaaacag agctcctgcc ctcctgaaaa ctgcaaagaa
961 aggggcgtgg cttcctgagt tcaaatccca actctgccag cgactagctg tacatcagtg
1021 atgtttccct actttctctc aattaaatag ggataatgtc agtacctatc acattgggag
1081 gtcttgcggg gattaaatga gttaccaaat gccaagtgtt tgggacaggg cctggcaccc
1141 agcaaagtct cttgtgagtg ctggctgcta ttatcctaat ggagaagatg gcatgaaaac
1201 caggaaatag gatgcccttt gggaagcaat gcaacaggaa cttacacaaa gaaaggaaag
1261 gaggaagcaa ttagtggtgt ctcaaaggag tatgtcaaga aaaacttttc agagggaaac
1321 ctttgagcag ggtcatgaaa acaggagttc tctaagagat tgtggacttg cctgggacca
1381 cctggctata agcacaaaac catccggttc ctttctgtca cttctggcgg gtgaggggtc
1441 tctggcaaag gggcagaagg tgcgtgagag gttgcgaatg gccaggactg tcctggggcc
1501 agccggggca cctggtggcc aagcttagaa acatgacagg tcctcttggg agggctgacc
1561 gcagggagcg ttgggtttca ggctgctggc gtcggcttct gtggtgccct ttctgtcggc
1621 tatgagagtc cagacagtgc ccaacctcct ccccttcttt ccacacgcac aaccacccca
1681 ccccctgtgg cctgagctgt cctgcctcgc cacaatggca cctgccctaa aatagcttcc
1741 catgtgaggg ctagagaaag gaaaagatta gaccctccct ggatgagaga gagaaagtga
1801 aggagggcag gggaggggga cagcgagcca ttgagcgatc tttgtcaagc atcccagaag
1861 gtataaaaac gcccttggga ccaggcagcc tcaaacccca gctgttgggg ccaggacacc
1921 cagtgagccc atacttgctc tttttgtctt cttcagactg cgccatgggg ctcagcgacg
1981 gggaatggca gttggtgctg aacgtctggg ggaaggtgga ggctgacatc ccaggccatg
2041 ggcaggaagt cctcatcagg taaaaggaag agattccatt gcccctgcca cccacaccct
2101 aagatcaagg gtgttcagct gcaaggtgga aagtttgcac gtggggtagg tcagttggct
2161 gcattagtta agggtgttag aacggtcact tgctttttct ttgcttttaa gtgtcaggga
2221 ttggactcag gagagggaaa ggagccattt caggctgatg tcagcagctg gaggaagcat
2281 gagaatcaaa cctaggatgc tcagagtcca ccaggaagaa ttttagaatt atagacagtc
2341 agagttaaca agggtcctga gagattttgt acagccacct ctcttacagg atgaggacaa
2401 aaagcgactg agaaggggag gacatttcca gagtcacagc tcattaaatg ctcttaaagt
2461 gtcaaggtta agacatgctc ttcaagggga gacagatctg gttctagact tggctctgcc
2521 actgagccac tgggtgacct ttgggaaggt ac
Official Symbol MBprovided by HGNC
Official Full Name myoglobinprovided by HGNC
Primary source :HGNC:6915
Locus tag :RP4-569D19.7
Seerelated：Ensembl:ENSG00000198125; HPRD:01170; MIM:160000; Vega:OTTHUMG00000150606
Gene type:protein coding
Refsep status:REVIEWED
Organism：Homo sapiens
Lineage： Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as：PVALB
Genomic context
Location:
22q13.1
Sequence:
Chromosome: 22; NC_000022.10 (36002811..36019401, complement)
See MB in Epigenomics, MapViewer
Chromosome 22 - NC_000022.10

Genomic regions, transcripts, and products
GenomicSequence                                                   

Go to nucleotideGraphics FASTA GenBank

NC_000022.10: 36M..36M (22Kbp) C

Find on Sequence:

  -  

  +  

Tools

Configure

2. 利用Primer premier5.0软件对myoglobin核酸序列进行引物设计其结果如下：
Sense primer:
5' TACTCGTGCCTTTCGTACTAGGCTC 3'

Anti-sense primer:
5' ATGAGCACGGAAAGCATGATCCGAG 3'
3. 蛋白质序列分析
利用ExPASy软件包对该基因进行氨基酸组成统计、分子量、等电点等分析及磷酸化分析