AKTA纯化系统培训指南 英文版.pdf

KTAKTAexploreramersham bioscienceamersham bioscienceamersham bioscienceamersham bioscienceKTA system Training GuideExplorer 100/10KTAKTAexploreramersham bioscienceamersham bioscienceamersham bioscienceamersham bioscienceBoxMonitorConductivitypHUV Detector3 WavelengthsPumpsColumnValvesInjectorSampleValveBufferspH/cellelectrodeSampleholderPump A Buffer switchingvalveOutletValveKTAexplorer23456781B4ASystempumpsMixerFractioncollectorFrac-950ConductivitymonitorpH detectorIn-linefilterSamplepumpBuffer selectvalve(V6)Injectionvalve(V1)Reverseflow valve(V7)Outletvalve(V4)Sample selectionvalve(V5)Columnselectionvalve(V2)Columnselectionvalve(V3)3 Wavelengthdetector2345678123456781234567812345678124731652473165UVCondpHKTAexplorer 100 Airsensor23456781B4ASystempumpsMixerFractioncollectorFrac-950ConductivitymonitorpH detectorIn-linefilterSamplepumpBuffer selectvalve(V6)Injectionvalve(V1)Reverseflow valve(V7)Outletvalve(V4)Sample selectionvalve(V5)Columnselectionvalve(V2)Columnselectionvalve(V3)3 Wavelengthdetector2345678123456781234567812345678124731652473165UVCondpHKTAexplorer 100 Air23456781B4ASystempumpsMixerFractioncollectorFrac-950ConductivitymonitorpH detectorIn-linefilterBuffer selectvalve(V6)Injectionvalve(V1)Outletvalve(V4)Columnselectionvalve(V2)Columnselectionvalve(V3)3 Wavelengthdetector234567812345678123456781UVCondpHKTAexplorer 10 23456781B4ASystempumpsMixerFractioncollectorFrac-950ConductivitymonitorpH detectorIn-linefilterSamplepumpBuffer selectvalve(V6)Injectionvalve(V1)Outletvalve(V4)Sample selectionvalve(V5)Columnselectionvalve(V2)Columnselectionvalve(V3)3 Wavelengthdetector23456781234567812345678123456781UVCondpHKTAexplorer 10 S24731652473165KTAexplorer 10 XT 23456781B4ASystempumpsMixerFractioncollectorFrac-950ConductivitymonitorpH detectorIn-linefilterBuffer selectvalve(V6)Injectionvalve(V1)Outletvalve(V4)Columnselectionvalve(V2)Columnselectionvalve(V3)3 Wavelengthdetector234567812345678123456781UVCondpH2473165PUMP P-900Buffer Acid/AlkaliWaterSaltProportion ValvePump AMixerA2A1B2B1Pump BGRADIENTMIXER KTA 10 mls/min6 mls/min6-14 mls/min0.6 ml mixer2 ml mixer KTA 100 mls/min30 mls/min30-50 mls/min50-100 mls/min2 mls mixer5 mls mixer12 mls mixerPUMP P-900 InletsWaterSaltBuffer Select Valve IV-908Pump APump BMixerA2A1B1B2SwitchingValves12345678A11A12A13A14A15A16A17A18PUMP P-900 Manually Priming Buffer Inlet LinesOpenDraw Air out ofBuffer LineManual FlowpathA1 or A2BufferValveAManual FlowpathB1 or B2 BufferValveBPump APump BCommon inletA2A1B2B1Common inletPUMP P-900 Back-Wash Lines20%Ethanol:WaterPump Back-WashLinesWash OUTWashINBufferINBufferOUTHigh pressurePiston sealBall outletcheck valveBall inletcheck valvePistonBackwash pump diaphragmIN-LINE one-wayvalveSyringe fittingLOAD(sample)INJECT(sample)LOAD(wash loop)1765234PumpColumnWastePV-90812345678Sample1765234PumpColumnWastePV-90812345678Sample1765234PumpColumnWastePV-90812345678SampleON-LINE FILTERSP-901(100 ml/min Pump)High Flow Fibre FilterPolymer SupportP-903(10 ml/min Pump)Low Flow HPLC Filter1765234PUMPLOADSAMPLEWasteCOLUMNWasteLOAD1765234PUMPWasteCOLUMNWasteINJECT1765234PUMPWasteCOLUMNWasteWASTEINJECTION VALVE INV-907SAMPLE LOADING METHOD1765234PUMPLOADSAMPLEWasteCOLUMNWasteSuperloopLuer LockAdapter1765234PUMPLOADSAMPLEWasteCOLUMNWasteNeedle PortAlways tighten by hand with needle insertedDo Not OvertightenVolumeMax.allowed column pressureCode No.10 ml4 Mpa18-1113-8150 ml4 Mpa18-1113-82150 ml2 Mpa18-1023-85SUPERLOOPS10 ml150 ml50 mlBUFFERFLUSHSAMPLELOADSAMPLEINJECTSAMPLEFLUSHWASTEPUMP orSYRINGECOLUMNCOLUMNPlungerBufferSampleEtchedGrooveNeedleNeedleAdapterINV-907 Injection Valve-Injection PortPEEK Valve BodyValve SealInjection Needle PortNeedleTubeFerruleINV-907 Injection Valve-Luer AdapterPEEK Injection Valve BodyPEEK Injection Valve BodyPEEK Injection Luer AdaptorPEEK Injection Luer AdaptorValve SealValve SealValve Channel Plate Valve Channel Plate FlowEndBeginningPEEK Tubing High Resistanceto FlowLow Resistanceto FlowFluid DynamicsFlow-Injecting a LoopFluid DynamicsGeneralThe way we imagine it FlowsThe way it Actually FlowsBubble SegmentationPartial Loading Loop =No more than 1/2 Loop VolumeTotal Loading Loop =Inject 2X Loop Volume50ul Load60ul Load100ul Load200ul LoadWaste100ul LoopLOADING A SAMPLE LOOP1765234SamplePumpColumnInjectLoadSamplepumpP-950Injection valveINV-907WasteLOADING A SUPER LOOP1765234SamplePumpColumnInjectLoadWasteSampleBufferInjection valveINV-907MULTI-LOADING A SAMPLE LOOP1765234PumpInjectLoadColumnPV-908Samples12345678I/OInjection valveINV-907WasteWashMULTI-LOADING A SUPER LOOPWaste1765234PumpColumnInjectLoadPV-90812345678I/OWashSamplesS3 S4 S5 S6S8Injection valveINV-907DIRECT LOADING ON TO COLUMNPumpColumnExtra TubeInjectLoad1/16”or 1/8”TubingSample1765234WasteREVERSE FLOW VALVE (INV-907)ColumnPumpDownward FlowPosition 1Extra TubeMONITORStopPlug17652341ColumnExtra TubeMONITORStopPlug2Upward FlowPosition 2Pump76534OUTLET VALVE PV-908DefaultTo Fraction CollectorDETECTORF1F2F3F4F5F6F7F8I/OWasteF3F5F6F7F8F40%B10050EluateFractionationPeakFractionationFlowthroughFractionation0%B100%BStartEndA1 A2 A3.InjectmAU0500250WasteFraction CollectionHiTrap ConnectionsM6 Male1/16”Female1/16”Male1/16”FemaleM6 MaleM6 Female1/16”MaleM6 male/1/16”Female18-1112-5718-1112-55Flow Cell InletHiTrap Column18-1112-58M6 female/1/16”male1/16”Fingertight FittingPhenyl SepharoseDetectorMultiple HiTrap Arrangment(Series)Phenyl SepharoseDetectorPhenyl SepharosePhenyl SepharoseDetectorPhenyl SepharosePhenyl SepharosePhenyl Sepharose