穴位肥大细胞及其颗粒物在针刺镇痛中的作用.pptx

1纪念安徽针灸经络研究纪念安徽针灸经络研究5050周年周年 穴位肥大细胞及其颗粒物在针刺镇痛中的作用针灸经络研究回顾与展望国际学术研讨会针灸经络研究回顾与展望国际学术研讨会2010.10.162Outline&穴位肥大细胞在针刺镇痛效应中的作用&穴位胶原纤维在针刺镇痛效应中的作用&穴位注射组胺的镇痛效应&手针和电针穴位信号的启动机制Mast cells in acupoints and sidewardComparison of Number of MCs in Zhushanli and non-acupoints of RatGroup NameGroup NameMCs NumberMCs NumberSkinSkinMuscleMuscleAcupointAcupoint（n n n n6 6 6 6）34.22.534.22.512.41.412.41.4Non-acupointNon-acupoint（n n n n6 6 6 6）22.11.7*22.11.7*8.51.0*8.51.0*THE JOURNAL OF SCIENCE&HEALING,2008THE JOURNAL OF SCIENCE&HEALING,2008，4 4（3 3），），170-177170-1771.The relationship betweenmast cell function and acupuncture effectAnimal modelAnimal model ：AA AA (acute arthritis)(acute arthritis)RatRatComplete Freunds adjuvant Acupoint selectedAcupoint selected：Zushanli Zushanli (ST36ST36)D DSTST 3636C C1.The relationship betweenmast cell function and acupuncture effectTreat effectTreat effect：Ease painEase pain，Pain Pain thresholdthreshold1.The relationship betweenmast cell function and acupuncture effectThe degranulation of mast cells A.SD RatsSD RatsB.Tail Flick ModelTail Flick ModelC.Anatomy Position of Anatomy Position of ZusanliZusanliD.AcupunctureAcupunctureE.Injection drugs for prevent Injection drugs for prevent degranulation of MCdegranulation of MCA AB BD DE ESTST 3636C CPain threshold of rat when shield mast cell degranulation1.The relationship betweenmast cell function and acupuncture effectIDGroup Name组别组别Average SEM(%)AControl正常对照组正常对照组1.90.1B1Acupuncture at ST36穴位针刺组穴位针刺组11.60.6*B2Acupuncture at Nearby-ST36旁开针刺组旁开针刺组8.90.3*C1Injection of DSCG at ST36色甘酸钠注射组色甘酸钠注射组1.70.1*C2Injection of NaCl at ST36生理盐水注射组生理盐水注射组1.40.1*D1Acupuncture after DSCG at ST36色甘酸钠注射色甘酸钠注射针刺组针刺组6.20.4*D2Acupuncture after NaCl at ST36生理盐水注射生理盐水注射针刺组针刺组8.90.4*D3Acupuncture opposite side after DSCG色甘酸钠注射色甘酸钠注射对侧针刺组对侧针刺组9.30.1*各组别大鼠痛阈变化率峰值比较（各组别大鼠痛阈变化率峰值比较（2020分钟时）分钟时）(n=6)(n=6)Note:*P0.05 vs.Group A;P0.01,P0.01,P0.05 vs.Group B1;P0.05 vs.Group B1;P0.01,P0.01,P0.05 vs.Group D1P0.05 vs.Group D11.The relationship betweenmast cell function and acupuncture effectGroup Name组别组别MC Degranulation Ratio(Average SEM)()SkinMuscleControl正常对照组正常对照组29.21.226.31.8Acupuncture穴位针刺组穴位针刺组48.93.9*32.23.1*Acupuncture after DSCG色甘酸钠注射针刺组色甘酸钠注射针刺组33.72.828.52.3Acupuncture after NaCl生理盐水注射针刺组生理盐水注射针刺组50.42.5*29.72.3穴区组织肥大细胞脱颗粒率统计穴区组织肥大细胞脱颗粒率统计 (n=6)(n=6)Note:*P0.01,*P0.05 vs.Control Group;P0.01 vs.Acupuncture Group.P0.01 vs.Acupuncture Group.Results from ExperimentStaining of Mast Cells in Acupoint on RatsMusclMuscle eSkinSkinBefore Before AcupunctureAcupunctureAfter After AcupunctureAcupunctureDegranulationof cellsAfter SDCG After SDCG StabilizedStabilizedTHE JOURNAL OF SCIENCE&HEALING,2008THE JOURNAL OF SCIENCE&HEALING,2008，4 4（3 3），），170-177170-177What is the key factor to degranulation lThe acupuncture effect is positive relationship to the degranulation of mast cell at acupoint.lWhat is key factor conduce the degranulation of mast cell during acupuncture manipulation?lOur focus is on the collagen at acupoint.2024/8/23 周五周五13To observe the function of collagen fibers in MA analgesia.7 Animal groups setting:normal group(N):Normal healthy animal control group(M):Animal with AA injection of collagenase at Zusanli(ST36)group(C)manual acupuncture group with rotation manipulation(R)or lift-thrust manipulation(L),rotation group with collagenase pretreatment to destroy Zusanli(ST36)collagen fibers(CR),lift-thrust group with collagenase pretreatment to destroy Zusanli(ST36)collagen fibers(CL),lift-thrust group pretreated with collagenase to destroy Dubi(ST35)collagen fibers(CDL)(R)(L)2024/8/23 周五周五14Analgesia effects observed in different groupsGroupnPain Threshold(PT)4d Before MA(s)After MA(s)Change Rate(%)MC Degranulation Ratio(%)N89.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.94.7 R85.440.37*7.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85.270.62*5.390.573.310.42.4 CL86.050.43*6.260.793.812.42.3 注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处理前痛阈：P0.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插组:P0.05 P0.05。2024/8/23 周五周五15Analgesia effects observed in different groupsGroupnPain Threshold(PT)4d Before MA(s)After MA(s)Change Rate(%)MC Degranulation Ratio(%)N89.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.94.7 R85.440.37*7.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85.270.62*5.390.573.310.42.4 CL86.050.43*6.260.793.812.42.3 注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处理前痛阈：P0.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插组:P0.05 P0.05。2024/8/23 周五周五16Analgesia effects observed in different groupsGroupnPain Threshold(PT)4d Before MA(s)After MA(s)Change Rate(%)MC Degranulation Ratio(%)N89.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.94.7 R85.440.37*7.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85.270.62*5.390.573.310.42.4 CL86.050.43*6.260.793.812.42.3 注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处理前痛阈：P0.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插组:P0.05 P0.05。collagen fibers of skin and muscleMast cells in skinGroupNormalGroupInjectControlcollagen fibers of skin and muscleMast cells in skinConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120Acupunc.whirling Acupunc.lift and thrust collagen fibers of skin and muscleMast cells in skincollagen fibers of skin and muscleMast cells in skinConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120 Mast cells degranulation before and after collagen destroyed Collagen destroyedCollagen destroyedConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120collagen fibers and mast cells in normal acupointcollagen fibers enlacement and mast cells degranulation while whirlingcollagen fibers tightened and mast cells degranulated while lifting and thrustingcollagen fibers were destroyed and mast cells had no degranulationConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-1202024/8/23 周五周五21The mast cells in acupoint may translate the mechanical signal into the biological information of neural system.The collagen and mast cells degranulation are very important to the manual acupuncture analgesia effect.Is this the same as electric acupuncture?2024/8/23 周五周五22组别组别n 痛阈痛阈(s)痛阈变化率痛阈变化率(%)肥大细胞脱颗肥大细胞脱颗粒率粒率(%)正常组正常组8 9.190.65-10.6模型组模型组8 5.610.48*-14.1手针组手针组8 7.520.6538.5 75.4电针组电针组8 8.671.0645.9 73.3胶原酶胶原酶+手针组手针组8 5.390.573.2 10.4胶原酶胶原酶+电针组电针组8 8.301.2347.6 19.0色甘酸钠色甘酸钠+手针组手针组8 6.000.485.8 25.1色甘酸钠色甘酸钠+电针组电针组8 7.340.7027.5 29.5手针及电针治疗前后手针及电针治疗前后AAAA大鼠痛阈和痛阈改变率以及穴大鼠痛阈和痛阈改变率以及穴区肥大细胞脱颗粒率的变化区肥大细胞脱颗粒率的变化注：与注：与 正常组相比较：正常组相比较：*P0.05P0.05；与；与 模型组相比较：模型组相比较：P0.05P0.05；与手针组与手针组相比较：相比较：P0.05 P0.05；P0.01 P0.01；与电针组相比较：；与电针组相比较：P0.05P0.05。3.穴位皮下注射组胺的镇痛疗效穴位皮下注射组胺的镇痛疗效组别组别n 痛阈痛阈(s)基础痛阈基础痛阈造模后痛阈造模后痛阈治疗后痛阈治疗后痛阈空白对照组空白对照组129.040.209.380.199.270.17模型组模型组128.900.406.480.286.580.35生理盐水对照组生理盐水对照组129.200.286.090.306.610.31手针组手针组129.520.186.580.178.770.26组胺注射组组胺注射组129.210.206.270.228.500.28各组痛阈测量比较各组痛阈测量比较3.穴位皮下注射组胺的镇痛疗效穴位皮下注射组胺的镇痛疗效组别组别n 痛阈痛阈(s)基础痛阈基础痛阈造模后痛阈造模后痛阈治疗后痛阈治疗后痛阈空白对照组空白对照组129.040.209.380.199.270.17模型组模型组128.900.406.480.286.580.35生理盐水对照组生理盐水对照组129.200.286.090.306.610.31手针组手针组129.520.186.580.178.770.26组胺注射组组胺注射组129.210.206.270.228.500.28各组痛阈测量比较各组痛阈测量比较针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-1033.穴位皮下注射组胺的镇痛疗效穴位皮下注射组胺的镇痛疗效组别组别n 痛阈痛阈(s)基础痛阈基础痛阈造模后痛阈造模后痛阈治疗后痛阈治疗后痛阈空白对照组空白对照组129.040.209.380.199.270.17模型组模型组128.900.406.480.286.580.35生理盐水对照组生理盐水对照组129.200.286.090.306.610.31手针组手针组129.520.186.580.178.770.26组胺注射组组胺注射组129.210.206.270.228.500.28各组痛阈测量比较各组痛阈测量比较针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-1033.组胺组胺H1受体在传递针刺信号中的作用受体在传递针刺信号中的作用 各组足三里穴位处脱颗粒比较各组足三里穴位处脱颗粒比较组别组别N脱颗粒率脱颗粒率空白组空白组121233.5933.590.72模型组模型组121239.7139.712.09 生理盐水穴位注射组生理盐水穴位注射组121236.6736.672.36手针针刺组手针针刺组121257.6157.611.42组胺穴位注射组组胺穴位注射组121257.0357.032.95色甘酸钠预处理胺注射组色甘酸钠预处理胺注射组131325.4025.401.80色甘酸钠预处理针刺组色甘酸钠预处理针刺组121236.0336.032.28组胺组胺H1拮抗剂预处理针刺组拮抗剂预处理针刺组121251.5451.542.32组胺组胺H1拮抗剂组胺注射组拮抗剂组胺注射组121237.1337.131.90组胺组胺H1拮抗剂穴位注射组拮抗剂穴位注射组222232.2432.241.40P0.01 P0.01 与空白组比较（与空白组比较（vs.controlvs.control）；）；P0.01 P0.01 与模型组比较（与模型组比较（vs.modelvs.model）；）；P0.01 P0.01 与手与手针针刺组比较（针针刺组比较（vs.controlvs.control）；）；P0.01 P0.01 与生理盐水穴位注射组比较；与生理盐水穴位注射组比较；P0.01 P0.01 与组胺穴位注射与组胺穴位注射组比较，组比较，P0.01 P0.01 与组胺与组胺H1H1拮抗剂穴位注射组比较。拮抗剂穴位注射组比较。针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-1033.组胺组胺H1受体在传递针刺信号中的作用受体在传递针刺信号中的作用 各组足三里穴位处脱颗粒比较各组足三里穴位处脱颗粒比较组别组别N脱颗粒率脱颗粒率空白组空白组121233.5933.590.72模型组模型组121239.7139.712.09 生理盐水穴位注射组生理盐水穴位注射组121236.6736.672.36手针针刺组手针针刺组121257.6157.611.42组胺穴位注射组组胺穴位注射组121257.0357.032.95色甘酸钠预处理胺注射组色甘酸钠预处理胺注射组131325.4025.401.80色甘酸钠预处理针刺组色甘酸钠预处理针刺组121236.0336.032.28组胺组胺H1拮抗剂预处理针刺组拮抗剂预处理针刺组121251.5451.542.32组胺组胺H1拮抗剂组胺注射组拮抗剂组胺注射组121237.1337.131.90组胺组胺H1拮抗剂穴位注射组拮抗剂穴位注射组222232.2432.241.40P0.01 P0.01 与空白组比较（与空白组比较（vs.controlvs.control）；）；P0.01 P0.01 与模型组比较（与模型组比较（vs.modelvs.model）；）；P0.01 P0.01 与手与手针针刺组比较（针针刺组比较（vs.controlvs.control）；）；P0.01 P0.01 与生理盐水穴位注射组比较；与生理盐水穴位注射组比较；P0.01 P0.01 与组胺穴位注射与组胺穴位注射组比较，组比较，P0.01 P0.01 与组胺与组胺H1H1拮抗剂穴位注射组比较。拮抗剂穴位注射组比较。针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103穴位处组胺及穴位处组胺及H1受体受体l组胺参与到了针刺麻醉的穴位局部机制组胺参与到了针刺麻醉的穴位局部机制l通过通过H1受体进一步传递针刺信号受体进一步传递针刺信号研究阐明了手针和电针穴位针刺有效信号的启动机制研究阐明了手针和电针穴位针刺有效信号的启动机制MAEAElectric A.Thank you