Milli-Q Advantage A10 维护使用.ppt

单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,Milli-Q,Advantage A10,超纯水系统的维护与使用,Millipore,水系统总图,Water Type,Daily Use and Systems,Type 1,Type 2,Type 3,Tap,5 25 200 2000,Volume in Liter/day,Milli-DI,Milli-Q,RiOs,Elix,Simplicity,Synergy,Direct-Q,L RiOs,L Elix,Super-Q,实验室用水等级的划分,实验室用水的特点,最少的污染，可更好的控制实验的条件,不同的组织根据自身的要求，制定了不同的标准,各标准组织:,ASTM,:American Society for Testing and Materials,美国材料标准协会,NCCLS,:National Comity for Clinical Laboratory Standards,临床标准实验协会,CAP,:College of American Pathologists,美国病理协会,ISO,:International Standard Organization,国际标准组织,USP,:United States Pharmacopoeia,美国药典,EP,:European Pharmacopoeia,欧洲药典,中国国家标准-实验室用水（,GB668292）,例如,中国国家标准对于实验室用水的说明,（,GB668292）,（92年制定）,名称,一级,二级,三级,pH,值范围（25,0,C）,-,-,5.0-7.5,电导率(25,O,C),mS/m.,0.01,0.10,0.50,可氧化物质(以0计),mg/L ,0.08,0.4,吸光度(254,nm,1cm,光程),0.001,0.01,蒸发残渣105,O,2C,O,),mg/L ,1.0,2.0,可溶性硅(以,SiO,2,计),mg/L ,0.01,0.02,Millipore,推荐水质参数,超纯水系统的历史,The Ultrapure water system concept was invented by Millipore,密理博首先提出了超纯水系统的概念,The first system was the Super-Q,launched in 69,在1969年制造出第一台超纯水系统,Super-Q,The lab market was developed with the launch of the Milli-Q system in 1973,在1973年制造出第一台实验室超纯水系统,Milli-Q,Milli-Q water polisher promise to the customer:,low contamination,best verification,constant water quality,Milli-Q,系统提供的是最少的污染物,最好的认证和最稳定的超纯水.,Milli-Q,的发展历史(前六代),197319791982,198519891996,Milli-Q Century Range（,第七代）,Milli-Q,Gradient Synthesis Biocel Element,超低有机物型 综合型,Academic,超滤除热源型 痕量离子型,基础应用型,Milli-Q Advantage A10,NEW,est,!,Regular Lab,Applications,Chromatography,Applications,Millipak,Cell Culture,Applications,Molecular Biology,Applications,Biopak,产水水质,-,实验室用水,1,级水,(,超纯水,),Resistivity:,电阻率,18.2 M,.cm 25C,TOC:,有机物,5 ppb,Particulates:,颗粒物,1/ml if greater than 0.22 m(*)(*),Bacteria:,细菌,1 cfu/ml(*)(*),Pyrogens:,热源,0.001 Eu/ml(*),Rnases:RNa,酶,0.01 ng/mL(*),Dnases:DNa,酶,4 pg/L(*),(*)With Millipak-(*)With BioPak,Milli-Q,水实验室应用范围,LC,缓冲液制备,HPLC,等度和梯度洗脱液的配置,样品的配置和稀释,缓冲液和细胞培养基的制备,滴定，光谱，电泳系统中使用的化学溶液的制备,(*)With Millipak-(*)With BioPak,Milli-Q Advantage A10,水流路图,进水,:,进水电磁阀,Q-Gard T,柱,分配泵,进水电导率仪,(,可选,),流量计,A,10,r,r,中间电阻率仪,UV 185 nm,Quantum T,柱,产水电阻率仪,Q Pod 1,Q Pod 2,Pod Pak 1,Pod Pak 2,A10 TOC,检测仪,Milli-Q,系统纯化组件,Q-G,ard,预纯化柱,UV,紫外灯,Q,uantum,超纯化柱,UF,超滤柱,MF,过滤器,+,+,+,+,或,天然,人工合成活性炭,自来水预处理,Progard,QGard,Quantum,Q-Gard,和,Quantum,树脂,Characteristics,Size and Color Homogeneous,No particulates or fines,No grain cracked(single regeneration),As a result:,High capacity with good binding kinetics,Low organic release,离子交换树脂的再生,离子交换树,脂染色,:,Methyl Red(,阳离,子,),Bromophenol Blue(,阴离,子,),再生前,再生后,再生前后树脂比较,紫外,线,光氧化,的辐射波长,波長(,n.m.),185,n.m.,254,n.m.,相对辐射强,度%,100%,20%,杀菌波长,造成,光氧化,作用,的波长,氢,氧自由基,的循环途径,UV at,254,nm,+,H,2,O,H,2,O,2,+O,2,UV at,254,nm,1.5,O,2,UV at,185,nm,O,3,UV at,254,nm,+,H,2,O,O,2,+O,.,2,OH,.,氢,氧自由基,UV at,254,nm,水中有,机,物,的,光氧化途,径,CO,2,+H,2,O H,2,CO,3,HCO,3,-,+H,+,CH,3,OH+2,OH,.,HCHO+2 H,2,O,(甲醇),UV,HCHO+2,OH,.,HCOOH +H,2,O,(甲酸),UV,HCOOH+2,OH,.,CO,2,(aq),+2 H,2,O,UV,MF,，,UF,过滤及超滤,扫描电镜放大,2000,倍的,0.22,um,Durapore,滤膜表面,.,Milli-pak,表面过滤,0.5 mm,0.8 mm,Biopak-,中空纤维超滤柱,Cut Off(NMWL)=13,000 Dalton,电阻率仪,-,灵敏度高,i,s,s,L,I=V/R,Cell Constant:L/S,R=Resistance,=Resistivity,R=,r.,L/S,I=,V,r.,L/S,Cell Constant=L/S,Low cell constant=better accuracy:,higher signal and lower interferences,Voltage 1.2 V to avoid reactions at,the surface of electrodes,Low frequency 100 Hz to limit the,phenomena of resistance parasites,电阻率仪,-,灵敏度高,Concentric electrodes design(with collar)ensuring reproducible manufacturing,Made of high quality 316 L stainless steel,Low cell constant(0.01)for accuracy,No dead legs,Protected thermistor,Sensitivity 0.1 C,Electronic autotest,TOC,监测仪,-,检测原理,A water sample is trapped in the cell.,The UV Lamp irradiates it and ignites the photoxidation of the organics.,The electrodes continuously monitored the sample conductivity variation,185/254 nm,UV Lamp,Titanium,Electrodes,Solenoid,Valve,Thermistor,Quartz cell(0.5 mL),Water in,Water out,Oxidation Chamber,TOC,监测仪,-,检测原理,From US Patent N 4666860-1987,Conductivity,Purge,Measure of initial,conductivity,Oxidation,cycle,Purge,Phase 1,Phase 2,Phase 3,Phase 4,Valve open,Valve closed,Valve open,Lamp on,Lamp on,Lamp off,D,C,Time,取水流速,:,从逐滴到最大,2L/min,低流速,中等流速,高流速,(,同时锁定,),锁定连续流水后,将会在一定时间后自停止流水,.,此时间可设定修改,可由,QPOD,处使用真正的,”,定量取水,”,减少,增加,定量取水键,循环,打印,取水臂高度,角度可调,取水臂高度,角度可调,Qpod,显示屏信息,Quantum,UV,系统工作模式,Q-Gard,Qpod,显示屏信息,电阻率,(3,个模式,),TOC,(ppb),水箱液位,(%),定量取水体积,(L),Qpod,显示屏信息,维护提示,借误报警,定量取水指示,控制按键,导航键,确认键,类似手机按键,指示灯,重要,!,类似交通信号灯,正常,维护,报警,正常,系统可以正常使用，,但需要维护,系统不可以使用，需,要维修，请与,Millipore,联系,维护提示,The system guides the user,explaining which actions he should take,错误报警,The system guides the user,explaining which actions he should take,纯水耗材,Catalogue n,Description,List price(US$),Z00Q0V0T0,ZMQSP0D01,Milli-Q Advantage A10,Q-POD,QGARDT1X1,Q-Gard Pack with tag(1/pk),QTUM0TIX1,Quantum Cartridge(1/pk),MPGP02001,Millipak Express 20(1/4”Gaz-Straight),CDUFBI001,Biopak,ZMQUVLP01,UV lamp 185/254 nm,ZFA10UVM1,A10 UV lamp 185/254 nm,Elix/Rios,纯水系统的维护与使用,先进科技的组合,反渗,透膜,产水,ProGard,预纯化柱,自来水,EDI,模块,RiOs,Elix,自来水,预过滤,组件,UV,RiOs,水的应用领域,水浴摇床/水浴锅,灭菌器,玻璃器皿洗涤,Milli-Q,超纯水系统供水,4,种,RiOs,系统:,RiOs,3,RiOs,5,RiOs8,和,RiOs,16,最多每天可提供350升,III,级纯水,现有纯水系统的组成,-Total Solution,自来水预处理,+Rios+200,升水箱,+Milli-Q Advantage A10,=,整体解决方案,+,自来水预处理,深层过滤,活性碳过滤,软化器,RiOs,纯水系统,200,升水箱,+,北京希达普元公司提供,密理博公司提供,北京希达普元公司提供,自来水预处理,棉线滤芯,-1,个月更换,(,根据自来水水质,并查看颜色,),活性碳柱,-2,个月更换,(,根据自来水水质,),软化器,-,定期加盐,并,5,年更换树脂,(,根据实际使用情况,),深层过滤法,-,棉线柱所采用,深层过滤法,常用材质,Cotton Fibers,Glass Fibers,Polypropylene,Nylon Filaments,Sand Grains,孔隙度特性,Nominal,工作范围,0.5-1000,um,滤纸厚度,10-30,mm,30,um,玻璃纤维,1500,X,放大,活性炭吸附,适用原材料种类,椰子壳,木材,煤炭,原油,/,高分子材质,活化处理方法,加热处理,化学处理,经活化处理过的活性炭产生大量的,大/小,孔隙,使整体表面积大增,使表面吸附能力大增,活性炭可吸附的表面积,1000,平方米,/,克,活性炭内部结构,可有效中和漂白剂等强氧化剂,可大量吸附水中有机物,活性炭颗粒,天然,人工合成活性炭,Softener,-,软化,Ca,+,+2 Cl,-,Mg,+,+2 Cl,-,Na,R,Na,R,Na,R,Na,R,4,Na,+,+4 Cl,-,Ca,Mg,R,R,R,R,hard water”,硬水,soft water”,软水,ion exchange,离子交换,Softener,-,再生,Cl,-,Na,+,Na,R,Na,R,Na,R,Na,R,Mg,2+,+2 Cl,-,Ca,2+,+2 Cl,-,Excess Na,+,Cl,-,Na,R,Na,R,Na,R,Na,R,The resin counter ions are now Na,+,only.The resin is fully regenerated and ready for use again.,Commercial Softeners,Operation,Recycling,污染物去除效率比较,+,-,无机离子,有机物,颗粒物/胶体,微生物,可溶性气体,蒸馏,去离子,Elix,RiOs,Rios,系统的纯化顺序,RO,膜柱,可截留水中,99%,的污染物,Progard,纯化柱,预纯化作用,保护,RO,膜柱,Rios,产水,自来水预过滤系统,自,来,水,PROGARD,II PAK,抗结垢剂,颗粒状活性炭,(,去除有机物及氯,),(,防止碳酸钙结垢,),1,mm,Prefilter,(,去除悬浮微粒,),PAK LIFE,寿命确认,方法,按,MENU,键,2,秒,钟,OPERATE/STANDBY,MEASURE,CLEANING,MENU,PRES,：,450,Pa,50%,PRODUCT,：,3.8M,cm,SERVICE,：,LIFETIME,PAK.LIFE,：,25DAYS,例,)Progard Pack,仅剩,25,日或时间到,0,时,系统便会出现,“,EXCH.PACK,”,之,信息于屏幕上,进水,出水,反渗透膜的切向流技术,Feed Water,Permeate,Reject,RO Membrane,Tangential flow:to help avoiding quick membrane plugging but reject still needed!,Membrane,Permeate,Reject,Feed Water,Water Quality Ionic Rejection,截留率,Ionic rejection(%)calculation,:,feed,-,permeate,feed,Example,25C:,conductivity,=500 S/cm,conductivity,=25 S/cm,Ionic Rejection(in%)=100 x =95%,500-25,500,X 100,Permeate,Feed Water,Membrane,Reject,P,提高水的利用率,RO High Recovery Loop,A portion of the RO reject water is recycled back to the RO feed,Pump flow is constant flow,Back pressure regulating valve controls RO membrane pressure,Adjustable needle valve or capillary tubing controls reject flow rate to drain,Capillary tubing at low flows,(80 LPH),Recovery rates,up to twice as high,as typical RO-based systems,RiOs systems up to 70%,Elix up to 50%,For areas with good feed water conditions and/or additional pretreatment,Follow%Recovery Selection Guidelines,ELIX 5 UV,系统流路图（示意耗材位置）,0 自来水预过滤系统,9,单向阀 18 单向阀,1 进水电磁阀10 三向阀,2 ProGard,柱,11,EDI,模块,3,压力调节器12,EDI,产水电阻率仪,4,增压泵,13 单向阀,5,清洗口 14,RO,弃水电磁阀,6,进水电导率仪15 压力传感器,7,RO,膜,16 流量控制器,8 RO,产水电导率仪 17 毛细管,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,UV Module,254nm,SST housing,0,4,C,E,L,L,Pr,Elix/RiOs,系统的操作与维护,操作,Elix/RiOs,显示面板,指示灯,显示的内容,工作模式,提示信息,工作参数,查看,Progard,和,UV,灯寿命,系统维护,预过滤的更换,Progard,柱的更换,RO,膜的清洗,显示面板示意图,Keypad,液晶显示屏,操作/待机,状态,切换按键,工作参数,显示,清洗程序,启动按键,指示灯,控制按键,菜单功能设置按键,操作模式,（绿灯）,常规的工作模式,Standby,待机状态,在产水的模式下按住,Operate/Stanby,键2秒钟后系统会进入此状态。在此状态下可进行关机、系统维护等操作。在此状态下，周期性的冲洗程序依然会进行。,Operate,工作状态,在,Standby,的模式下按住,Operate/Stanby,键2秒钟后系统会进入此状态。是产水状态。系统清洗后会自动进入,Operate,状态。,Flush,冲洗,自动:冲洗反渗透膜(每6小时一次),Rinsing,清洗,自动:水质差的时候自动进入此清洗状态。,Tank Full,水箱已满,自动:水箱满了后系统自动进入此状态。,System Rinse,系统清洗,新系统安装或新反渗透膜更换后执行。,维护信息,（黄灯）,系统维护信息(黄灯闪烁),Start Autoclean.,RO,膜需要进行氯清洗.,自动提示,Exchange Pack/Tank Filter,Progard,已到寿命，需更换；水箱通气过滤器也需更换,自动提示,Cl,2,Clean,正在进行,RO,膜氯清洗程序(持续15 分钟),pH Clean,RO,膜(持续90 分钟,),Check pump,增压泵的性能低于应有标准,Exchange UV Lamp,UV,已到寿命，需更换。,自动提示,报警信息,（红灯）,报警信息,(红灯闪烁),Pack not in place,Progard,柱被拿开或没有正确安装，系统停机。,Low pressure,进水压力低于设定值。,Rejections.p.,RO,膜截留率小于设定值。,RO,膜需要更换。,Products.p.,E.D.I.,模块的产水电阻率小于设定值。,Error number?,故障编号。每个系统错误编号代表着不同的故障。,系统维护-更换,Progard,柱(1),当系统的黄灯闪烁并提示“,Exchange Pak”,时您需要更换,Progard,柱。,不要忘记水箱通气过滤器也要同时更换,用于密封的黑色橡胶圈,用于固定的孔,系统的氯清洗,当系统,黄灯,闪烁提示:,Start Autoclean,时系统需要进行氯清洗，耗时15分钟，是对,RO,膜的维护。,清洗周期,:,三个月,清洗步骤,:,切换到,Standby,状态，等待至,Standby,不再闪烁,用系统配备的工具逆时针打开清洗口,放入一片清洗药片,用手顺时针拧紧清洗口,按住,CLEANING,键2秒钟，此时显示,Cl2 Clean,系统开始15分钟倒计时，清洗结束后回到,Operate,状态,常见问题解答,以刻意添加有机物（蔗糖）于纯水中,作为,Milli-Q,的进水后,其所产制的超纯水的水质变化,0,30,60,90,120,150,180,210,240,用水时间累计,(,分钟,),8,10,12,14,16,18,20,电阻率,megohm-cm,0,200,400,600,800,1000,1200,有机物,(,TOC),ppb,电阻率,有机物,结论:电阻率并不能完全反映超纯水,MILLI-Q,纯化柱更换的依据,出水的电阻率:,当出水电阻率小于18,M,.cm,时,并持续下降,出水电阻率是18,M,.cm,但使用时间超过,1,年,:,树脂由于长时间浸泡,并有细菌等微生物的不断滋生,会使出水中的有机物等杂质含量很高.,TOC,值:(如有,TOC,在线测量仪),一般,TOC,大于,20ppb,时,有机物对树脂的影响,Bead,organic film,过多的有机物会裹附在树脂颗粒的表面,从而影响树脂的交换和活性,Milli-Q,系统对进水的要求,超纯水进水推荐指标：,Q-Gard 1：,电阻率：,10,s/cm,总有机碳：,1,m,颗粒物：,1/,ml,Q-Gard 2：,电阻率：,1,s/cm,总有机碳：,1,m,颗粒物：,1/,ml,推荐进水：,Elix,水、,RiOs,水、二级反渗透水、蒸馏水、一次反渗透水。,不推荐进水：,去离子水、来源不明的“蒸馏水”,超纯水的,pH,和电阻率,电阻率(,M,.cm),10,1,1,3,5,7,9,11,0.1,HCl,NaOH,pH,值,结论:超纯水即,R=18.2 M,.cm,水的,PH,值必定为,7.0,CO2,对超纯水的影响,Resistivity M,.cm,10-,20-,4-,0-,20,40,60,80,100,CO2,CO2+H20,H2CO3,HCO3 +H,CO3 +H,-+,=+,Air,At equilibrium,the concentration,of H,+,from H,2,CO,3,=2 x 10,-6,mole/L or pH=5.7,min,2,weeks,1,day,1,week,1,hour,Control,5,10,15,20,25,0,Minutes,超,纯,水,储,存在聚乙烯容器所,产,生,的,有,机,溶出物,Conditions,Water samples:40ml trace enrichment 2.0 ml/min,Column:C18 reversed phase(Waters),Gradient:linear 100%water to 100%acetonitrile,Wavelength:254 nm,我们提供专业的维修保养服务合同,包括内容,:,快速响应,电阻率仪，温度传感器的校准,耗材折扣,全管路更换,零配件的保修,具体请咨询,Millipore(400-889-1988),