限制性内切酶保护碱基表.pdf

PCRPCR 设计引物时酶切位点的保护碱基表设计引物时酶切位点的保护碱基表 切割率切割率%酶酶 寡核苷酸序列寡核苷酸序列 2 2 hrhr 2020 hrhr Acc I GGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 0 0 0 0 Afl III CACATGTG CCACATGTGG CCCACATGTGGG 0 90 90 0 90 90 Asc I GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 90 90 90 90 90 90 Ava I CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 50 90 90 90 90 90 BamH I CGGATCCG CGGGATCCCG CGCGGATCCGCG 10 90 90 25 90 90 Bgl II CAGATCTG GAAGATCTTC GGAAGATCTTCC 0 75 25 0 90 90 BssH II GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 0 0 50 0 0 90 BstE II GGGT(A/T)ACCC 0 10 BstX I AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT 0 25 25 0 50 90 Cla I CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG 0 0 90 50 0 0 90 50 EcoR I GGAATTCC CGGAATTCCG CCGGAATTCCGG 90 90 90 90 90 90 Hae III GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA 90 90 90 90 90 90 Hind III CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG 0 0 10 0 0 75 Kpn I GGGTACCC GGGGTACCCC CGGGGTACCCCG 0 90 90 0 90 90 Mlu I GACGCGTC CGACGCGTCG 0 25 0 50 Nco I CCCATGGG CATGCCATGGCATG 0 50 0 75 Nde I CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC 0 0 0 0 75 75 0 0 0 0 90 90 Nhe I GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG 0 10 10 0 25 50 Not I TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA 0 10 10 25 25 0 10 10 90 90 Nsi I TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT 10 90 90 90 Pac I TTAATTAA GTTAATTAAC CCTTAATTAAGG 0 0 0 0 25 90 Pme I GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG 0 0 0 75 0 25 50 90 Pst I GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT 0 10 90 90 0 0 10 90 90 0 Pvu I CCGATCGG ATCGATCGAT TCGCGATCGCGA 0 10 0 0 25 10 Sac I CGAGCTCG 10 10 Sac II GCCGCGGC TCCCCGCGGGGA 0 50 0 90 Sal I GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA 0 10 10 0 50 75 Sca I GAGTACTC AAAAGTACTTTT 10 75 25 75 Sma I CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 0 0 10 90 10 10 50 90 Spe I GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG 10 10 0 0 90 90 50 50 Sph I GGCATGCC CATGCATGCATG ACATGCATGCATGT 0 0 10 0 25 50 Stu I AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT 90 90 90 90 90 90 Xba I CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA 0 90 75 0 90 90 CTAGTCTAGACTAG 75 90 Xho I CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG 0 10 10 0 25 75 Xma I CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA 0 25 50 90 0 75 90 90 ApaI （类型：Type II restriction enzyme ）识别序列：5GGGCCC 3 BamHI（类型：Type II restriction enzyme ）识别序列：5 GGATCC 3 BglII （类型：Type II restriction enzyme ）识别序列：5 AGATCT 3 EcoRI（类型：Type II restriction enzyme ）识别序列：5 GAATTC 3 HindIII（类型：Type II restriction enzyme ）识别序列：5 AAGCTT 3 KpnI （类型：Type II restriction enzyme ）识别序列：5 GGTACC 3 NcoI （类型：Type II restriction enzyme ）识别序列：5 CCATGG 3 NdeI （类型：Type II restriction enzyme ）识别序列：5 CATATG 3 NheI （类型：Type II restriction enzyme ）识别序列：5 GCTAGC 3 NotI （类型：Type II restriction enzyme ）识别序列：5 GCGGCCGC 3 SacI （类型：Type II restriction enzyme ）识别序列：5 GAGCTC 3 SalI （类型：Type II restriction enzyme ）识别序列：5 GTCGAC 3 SphI （类型：Type II restriction enzyme ）识别序列：5 GCATGC 3 XbaI （类型：Type II restriction enzyme ）识别序列：5 TCTAGA 3 XhoI （类型：Type II restriction enzyme ）识别序列：5 CTCGAG 3