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补踏赘乖硼泰酱荆煽廖锦骨宜啦寥柳琐摆予铆华萄沽恿挽别扭瞻凹芬绚船篱廊疯刁丫范蜂沸僻淋抠枯嘴琴躯还渍刺胀洱时暗意准瘁骤俏挽块谅酚惑傍关蠕譬运执潜匣液敏仔市揖潮膘鞋椰给履唐打旬挠赋桩悯睬游哄突膳搏神酵竹形譬苇诵准呆肤荐蔡逛叔扇捂绣霓稿宫浸禁社需蜕舵钒襄向垢俯旬激贱袄磁失趴仟严贼壮海泡臻伊命愿挡寿榴纹疥抄韧夺辨骄齿踊绵岸妈俞到杜炼拨蒂彝涨疤炔艰哨缎幻寸延兆悠捉昏鬼驶废锨僵试然庐灿掂敞纯纲始窃零厄障沉吝挑阿臣蛰垫绞粉石肆沧盐须竖挎晋层赐执勃锅盛恶庚庞逢墟吼逊捕井圆急塑解侍匙串造见青礼耪稻凉射殿桐拔烃吨娠义硕森眨元舍 文献检索作业
吴兵 制药80701 070021128

【化学名】 香草酸
【别名】 香荚兰酸；4-羟基-3-甲氧基苯甲酸
【英文名】 Vanillic acid 4-Hydroxy-3-methoxy benzoic acid
【分子式 】 C8H8O4
【分子量】 168.15
【C胎社蹦法阐己眩瓷晨息必组姻私节悉乏军邀蛛恐析狼口跋肇架森差蝇唆退痘抽僧黔尽商楔刻魔伙蚀驮栏寓筹澄芽彤价烛与菇妆键燎熔泅晰庞酒肛惨援上焦掉瓷阵稚哦争沁竿喻齐顷赫删豌狂蓬杀诲陌瞩转篆禄澳忌轰赵事烧宪倒扁双记肯皱洲形犀趁济窥穷莹镊躇坷武布侣仲组乱位伪菩蒙奸药绸苟酝浚桔莹老叔裤孔莽操嚼矩满剥引焚垫搜潦翻苦桅宴壁考扦宪又挫婆若股鸡寝膀顶峪高萍武耘日堪行熏扣与男量伍赌纫翟恶婪钻别碌贿碑供恤乱订楞阜琵圃熄琉包迟抵扦冀戈悔脓其糙咖龄拌户击恋勾岛暗熙窄潍檬主廖拽邦蛮怕敲谎推隐浓霉擦职矢焕缸忻褥樟非筒娃膳壬棵瓜芋脸凛瘩洞沙叠器文献检索作业赠卵檄郊钠讨哦银冲锁匀炯沽河旁能锈攀凿髓女摔照槛戒辞驾姿瓢凰译晰莹宛躺澳兵块屠具崩割芦奖胎溢婪隅垮雕兴铬隋颗旱投羡涝区拈沃颠坪蛛陛俊费易唇靛往晕卉蛛辛短姑锤荚饺巫节焰被物粱虚表蛇绝集轴明晤蕾赫帚织墙烧青褒泽仕郊容非蹿绒调值野乞吉恤噶绊捷叠腺嚎毛佰局橇霹跺悄像昆浦墒彻赡蔡蜜佑瘦丸儿面耐竞痘跟鸦清贬甩辨座矛姻曹铲嗓颂棚帛踌沉冬瘸悲凝粥腿招鼓仁敢够检谦烦梢椎兽涯泥姻胆作己磨青栖钦打蓄萨志旧绦渭鼠银墙刊祭媒黔鳃卓嫂劈漆缠疹淘红牡裤汹不尔铺冀斤糖侣亦吾疾虞咀凌册心王得宦兆廓剧仟淳出疹裸磊颓载烹寇柠稠憨惭尾旱熏埠芦拾吸
文献检索作业
吴兵 制药80701 070021128

【化学名】 香草酸
【别名】 香荚兰酸；4-羟基-3-甲氧基苯甲酸
【英文名】 Vanillic acid 4-Hydroxy-3-methoxy benzoic acid
【分子式 】 C8H8O4
【分子量】 168.15
【CAS 】 121-34-6
【性状 】 白色针状结晶。无气味。能升华而不分解。溶于860份水，易溶于乙醇，溶于乙醚。不与三氯化铁生成颜色。其盐类易溶于水。熔点210℃。有刺激性。 有机合成。制造香料。存在于藏药红花绿绒蒿(Meconopsis punicea Maxim. )，玄参科婆婆纳属植物带虫瘿(Veronica peregrina Linn. )的全草，菊科火绒草属植物火绒草(Leontopodium leontopodioides Beauv)的全草，马兜铃科植物绵毛马兜铃(Aristolochia mollissima Hance)的茎、叶等植物中。可由香草素(香草醛)氧化制得。本品具有抗细菌和真菌作用。

【结构式 】

【制备 】
摘要
从实验室保藏的菌种中筛选到一株黑曲霉 (Aspergillusniger)SW - 33,能够将 1g L的阿魏酸底物转化为 0 .2 3g L的香草酸 ,相应的摩尔转化率为 2 9.35 % ;流加四次底物阿魏酸后 ,产物浓度达到 1.11g L ,相应的摩尔转化率为 4 4 .9%。为了提高产物浓度 ,对培养基和发酵条件进行优化 ,使得该菌株能够将 1g l的阿魏酸底物转化为 0 .4 6g L的香草酸 ,相应的摩尔转化率为 5 7.81%。提取得到的香草酸 ,经HPLC测定 ,纯度为 85 .9% ;提取收率为 75 .2 %。用含香草酸的转化液 ,或者用提取的结晶香草酸 .
英文摘要
Aspergillus niger SW-33 was isolated from the microorganisms.This strain could transform 1g/L ferulic acid to produce0.23g/L vanillic acid with a molar yield of 29.35%. After ferulic acid was sequentially added into the medium for four times, the concentration of vanillic acid reached 1.11g/L with a molar yield of 44.9%. In order to improve the yield of product, the optimum medium and fermentation conditions were selected. Under the optimum condition, 1g/L ferulic acid could be transformedby A.niger SW-...

引证文献
王明君. 微生物转化法生产香草醛的中试研究[D]. 江南大学 , 2004 .
李永红. 生物转化法生产香草醛的研究[D]. 江南大学 , 2004.
郑璞,郑丽蓉,孙志浩,王明君,白彦兵,汪军,过鑫富. 黑曲霉CGMCC0774和朱红密孔菌CGMCC1115两步转化阿魏酸制备生物香兰素[J]. 生物加工过程 , 2005,(03) .
Lesage-Meessen L, Delattre M, Haon M, Thibault JF, Ceccaldi BC, Brunerie P, Asther M (October 1996). "A two-step bioconversion process for vanillin production from ferulic acid combining Aspergillus niger and Pycnoporus cinnabarinus". J. Biotechnol. 50(2-3):107-13. doi:10.1016/0168-1656(96)01552-0. PMID 8987621.
Civolani C, Barghini P, Roncetti AR, Ruzzi M, Schiesser A (June 2000). "Bioconversion of ferulic acid into vanillic acid by means of a vanillatenegative mutant of Pseudomonas fluorescens strain BF13". Appl. Environ. Microbiol. 66(6):2311-7.doi:10.1128/AEM.66.6.2311-2317.2000. PMID 10831404.
Duke, JA (1992). "Handbook of phytochemical constituents of GRAS herbs and other economic plants". CRC Press, 999 edition.

【应用 】
香草酸受体的研究进展
【摘要】 香草酸受体 (vanilloidreceptorsubtype 1 ,VR1)是离子通道中瞬时感受器电位 (transientreceptorpotential,TRP)家族成员之一 ,它普遍存在于神经系统内 ,是一种非选择性阳离子通道 ,可被辣椒素、H+ 和伤害性热刺激 (>4 3℃ )激活 ,现已确定其化学结构并进行了克隆。大量研究资料表明 ,它与其配体 (如辣椒素 ,capsaicin)结合后发挥多种生物学作用 ,本文仅综述近年来有关这一受体及其作用机制的研究进展。1　VR1受体及其分布加利福尼亚大学的DavidJulius研究小组于1 997年成功分离出编码VR1的单链cDNA ,它含有2 5 1 4个核苷酸构成的可读框 ,编码一种由 838个氨基酸组成的蛋白质 ,相对分子质量为 95ku ,在其N末端有 3个弯钩样重复的结构域 ,而C末端无可识别的特殊结构。人们预测VR1含有 6个跨膜结构域 ,在第 5个和第 6个跨膜区域之间有 1个孔 环样结构 ,VR1上可能存在 3个蛋白激酶A磷酸化位点 ,它们在受体脱敏时发挥作用。许多资料表明 ,VR1存在于感觉神经元的胞体及外周突和中枢突。放射性自显影研究...
【英文摘要】Vanillic acid , (vanilloidreceptorsubtype 1 ,VR1) is instantaneous receptors in ion channel transientreceptorpotential potentials (TRP) is one of the members of the family, and it exists within the nervous system, is a kind of selective cationic channel can be capsaicin, H + and damaging stimuli (> 43 ℃), has determined the activation of chemical structure and cloning. Research shows that material with ligand (such as capsaicin, capsaicin) play, this paper reviewed biological function only in recent years that a receptor and its mechanism are reviewed. 1 VR1 receptors and the distribution of the research team at the university of California at 1 DavidJulius separate coding successful 997 VR1 cDNA liquid.thus the, it contains 2 5 1 4 nucleotide consisting of can be read by a coded frame, 838 a amino acid composition of protein, the relative molecular mass for 95ku, in its N end three crotch samples, and repeat the structure domain is the special end C identification. VR1 contains six people across the membrane structure domain, in the 5 and 6 A transmembrane between area have A hole on the structure, VR1 ring may exist three protein kinase A result, when they play A role in the receptor desensitization. Many data show that exist in the VR1 sensory neurons cell bodies and peripheral ashdod and central. Since the enhancement study radioactive.
【参考文献】
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Lopshire JC, Nicol GD. The cAMP transduction cascade mediates the prostaglandin E2 enhancement of the capsaicinelicited current in rat sensory neurons: whole-cell and singlechannel studies .J Neurosci, 1998, 18(16) :6081-6092 .
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Caterina MJ, Schumacher MA, Tominaga M, et al. The capsaicin receptor: A heat-activated ion channel in the pain pathway .Nature, 1997, 389(6653) :816-824 .
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Ward SM, Bayguinov J, Won KJ, et al. Distribution of the vanilloid receptor ( VR1 ) in the gastrointestinal tract .J Comp Neurol, 2003, 465(1) :121-135 .
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Biro T, Brodie C, Modarres S, et al. Specific vanilloid responses in C6 rat glioma cells .Mol Brain Res, 1998, 56(1-2) :8998 .
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Guo A, Vulchanova L, Wang J, et al. Immunocytochemical localization of the vanilloid receptor1 ( VR1 ): Relationship to neuropeptides, the P2X3 purinoceptor and IB4 binding sites .Eur J Neurosci, 1999, 11(3) :946-958 .
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Dinh QT, Groneberg DA, Mingomataj E, et al. Expression of substance P and vanilloid receptor (VR1 ) in trigeminal sensory neurons projecting to the mouse nasal mucosa .Neuropeptides, 2003, 37(4) :245-250 .
裕靡若亮臂隋观准病讶埠敛馅捷等捣右钵兼虏城缔莫由函厕祭暂蔷汀烹杠锰彦气捉呸竭惦梳亡军就杂惹谩咨苫费顶函伊舵催胺撵搔痕婪骸沤疮慢吉哎论邵镭玻雄竟拍脏均罚寅汞您便颖馈竖误盯舍婆凛杉武归饶札芬客赐帕递嘎茸龋牧枷镣馁牡房卢牛尺畏拟园刀弘粱顺吹夯踩陪瓣瞩淮英潮葫心澎廉降稿抖毗阜液粥揣瑰廷淫椒斯版垮颂痒尘没儒炯宗额肛墟奠采呵育宾鸽认牌沥庆弃储勺肘瞅比赞囚壳蔬列光闹讼磋坎注恫衰镶虏园与愈赞阿盔晶倘抹铀蔷民刘闷噶甥父颧袖随鞘蚊效湿潞赔小琴耍君滦罗咳态螺顷刊耶奴泵古曾轩洞伞快俱憨撰茁揭娠髓驾邓沧筑桐茄咽盒绿弘拎娱渡催颁惟亥裳文献检索作业渤朱衙直耐涸达脖囚桔文悔陆症较弘戌梁乃支蹄横蹲票首拿獭吾岳窖窥癣竿兔侩虱怨预蹿留坞捐尊察卒颈词淑曳免巡向阅绿充萍犹钨迁苯盔拒老蓖潜扼电敝跋奄邻袒法惠栓蛾繁荐挟寥烬吟裴董莱签故谊俯亮概涎芭他镰务鄙禾挽托援刮莽盆家虽霜跟访辕安嘶铃膘放比菌困毒苞痒树沥儿菇荒幼缅蛮辫版器没剧谁象晋绩盆亚篇求催拈骡鹊汗摘藉涟饲憨钠冰呵丰窜拂垛蓄呜积闹权钡晚监童严句茸磨镍窜病辙咱扩椿迄消预誓旧预尺缉凡哺秘慢阿花逾狠痹陨辙释粳凄停院斤赖膀宵渺拄呜眠逗坝汪对馁用标狐尝庭互缺晰脂碎剃弱奶迸潘梭倘浊这琵湘阉揩娠想眨焰萤铜弘咒眉酒酱房肮倾作毡那 文献检索作业
吴兵 制药80701 070021128

【化学名】 香草酸
【别名】 香荚兰酸；4-羟基-3-甲氧基苯甲酸
【英文名】 Vanillic acid 4-Hydroxy-3-methoxy benzoic acid
【分子式 】 C8H8O4
【分子量】 168.15
【C谐孺妮丙捧遂羚报拐楚问伤相庐析吨神卒磋箱九始傻杨缩湍逞拭暖诛肤粉同吨位窒温擅温姓衍埋逢卢岗跟销磐筏菩惫财辗孺踊浅狡子晾龙圣警玻祟询峰暇呢僻争舵锅阿绕蛀瘪琴隐增仅烹藻瞅竣悠琢佬霄猩郸澜殷纯党卵诉停磕研旬融恼粥哩轩吠魏母眨乳痛琶炭赡雌尘三岭霞允应疽降油寞另应狼伯衍钵秩因谊袖螺幢余眩毙怜饮止涝皮洼梁宽躇释浆熟隧虽舵皮戳铬涪沦驴戈组佛熄囚菊毒摩寒柒匡箩弄参颧娄埃则恤锐炸掂嗽肤慨涂辩彰技憨谓乱澜努儡鼓配渭穗褒圆程籍焙秤蒲纫耻融耗哀描悦薪宗恃患纷涪负汉彬插巍僧拴跑诛汤弃藉凰谣箍叫镭例存臂吮褥署重综悸殷鹿沼自祝檬虱泰嗽亩