血府逐瘀胶囊对急性心肌梗死再灌注后心肌及内皮素.doc

如有你有帮助，请购买下载，谢谢！
血府逐瘀胶囊对急性心肌梗死再灌注后心肌
及内皮素

作者：侯霄雷, 李白翎, 赵雷, 黄盛东, 徐志云, 张冠鑫

【摘要】 目的：观察血府逐瘀胶囊对急性心肌梗死（ acute

myocardial infarction, AMI）再灌注后心肌组织及一氧化氮（nitric

oxide, NO）/一氧化氮合酶（nitric oxide synthase, NOS）体系和

内皮素 1（endothelin 1, ET 1）的影响，探讨其改善血管内皮功

能的机制。方法：Yorkshire 猪 45 只，随机分成假手术组、模型对照

组和血府逐瘀胶囊治疗组。开胸结扎左冠状动脉前降支 90 min 后，松

解 2 h 制备 AMI 再灌注模型。测定造模前后和再灌注后血清 ET 1 和

NO 的含量；冠状动脉造影观察心肌血流恢复情况；Western blot 分析

心肌组织内皮型一氧化氮合酶（endothelial nitric oxide synthase,

eNOS）和 ET 1 蛋白表达；病理学分析心肌组织形态变化。结果：血

府 逐 瘀 胶 囊 组 造 模 后 和 再 灌 注 后 血 ET 1 水 平 低 于 模 型 组

（P<0.01） 而 NO 表达水平明显高于模型组（P<0.01） 冠状动
， 。

脉造影提示治疗组冠状动脉血流通畅情况优于对照组，但结果没有统

计学意义（P＝0.253） Western blot 显示血府逐瘀胶囊组 eNOS 蛋白 。

表达较模型组有所提高，ET 1 蛋白表达则有所下降（P<0.01） 。

HE 染色及微血管密度分析显示与模型对照组相比，血府逐瘀胶囊组毛

细血管扩张程度明显减轻，心肌组织损伤明显改善，微血管密度明显

增加。结论：内皮细胞受损可能是无再流现象发生的重要机制之一，

血府逐瘀胶囊可能通过调控 NO 和 ET 1 基因表达来达到对 AMI 的治疗

1页

如有你有帮助，请购买下载，谢谢！

作用。

【关键词】 血府逐瘀胶囊; 内皮缩血管肽 1; 一氧化氮; 一氧

化氮合酶; 心肌梗死

Objective: To observe the effects of Xuefu Zhuyu Capsule

(XFZYC), a compound traditional Chinese herbal medicine, on

endothelin 1 (ET 1) release in myocardium and vascular

endothelium and nitric oxide (NO)/nitric oxide synthase (NOS)

system of swines after acute myocardial infarction (AMI) and

reperfusion, and to explore the action mechanisms of XFZYC in

improving the endothelium function.

Methods: Forty five Yorkshire swines were randomized into

3 groups: sham operated group, untreated group and

XFZYC treated group. A Yorkshire swine model of reperfusion in

AMI was established by ligation of left anterior descending

coronary artery for 90 min followed by 2 h relaxation. The

content of serum ET 1 and NO was measured by radioimmunoassay

before and after AMI and after reperfusion, respectively.

Twenty four hours after operation, all Yorkshire swines

underwent diagnostic coronary angiography to delineate coronary

arteries. The expressions of ET 1 and endothelial nitric oxide

synthase (eNOS) in myocardial tissue of ischemic area were

quantified with Western blotting. Microvessel density of the

2页

如有你有帮助，请购买下载，谢谢！

implanting sites was assessed by using HE staining.Results:

Compared with the untreated group, the levels of serum ET 1

after AMI and reperfusion were significantly decreased in

XFZYC treated group (P<0.01), while the NO levels after AMI

and reperfusion in XFZYC treated group were significantly

increased (P<0.01). There was no significant difference in

diagnostic coronary angiography between XFZYC treated group

and untreated group (P＝0.253). Western blotting showed that the

level of ET 1 in ischemic area in XFZYC treated group was lower

than that in the untreated group (P<0.01), while the eNOS

protein expression in XFZYC treated group was higher than that

in the untreated group (P<0.01). The results of HE staining

and microvessel density analysis of the implanting sites all

showed that the degree of telangiectasis was reduced, the

cardiac muscle damage was improved, and the density of

capillaries was increased obviously in XFZYC treated group as

compared with the untreated group.Conclusion: The endothelium

injury may be one of the important mechanisms for no reflow

phenomenon. XFZYC may reduce the no reflow by protecting

endothelium cells.

Keywords: Xuefu Zhuyu Capsule; endothelin 1; nitric

oxide; nitric oxide synthase; myocardial infarction

3页