粒细胞集落刺激因子对CD34骨髓干细胞在心肌内归巢的影响.doc

粒细胞集落刺激因子对 CD34+骨髓干细胞在
心肌内归巢的影响

作者：冯金华，李玉光，石刚刚，侯玉清，曹世平

【关键词】 干细胞；心肌梗死；归巢；粒细胞集落刺激因子

【 Abstract 】 AIM: To explore the possible effects of

granulocyte colonystimulating factor (GCSF) on the homing of

CD34+ bone marrow stem cell (BMSC) to myocardium. METHODS:

Seventeen adult mongrel dogs were randomly assigned to 4 groups.

Group 1 (GCSF and MI, n=5) were treated with 10 mg/(kg・d)

×5 d of GCSF injection sc and then myocardial infract operation;

group 2 (GCSF and sham operation, n=4), treated with 10 m

g/(kg・d)×5 d of GCSF injection sc and sham operation;

group 3 (nonGCSF and MI, n=4) were injected with 10 m

g/(kg・d)×5 d sc normal saline and underwent myocardial

infarct operation; group 4 (nonGCSF and sham operation, n=4)

were injected with 10 mg/(kg・d)×5 d sc normal saline

and get sham operation. Thoracotomy was performed on the left

thorax of each animal and left anterior descending artery was

exposed and separated about 1 cm. In group 1 and 3 the separated

arteries were occluded for 3 h and then reopened, while the other

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2 groups were not treated in this way. Every dogs received

catheterizing in right femoral artery into the root of aorta arch

and a topmodified pigtail catheter cannulating through left

jugular superficial vein into coronary venous sinus. 5 mL blood

from each animal aorta and coronary sinus were harvested for two

times, in which erythrocytes were lysed. Mononuclear cells were

separated with lymphocyte separating solution and CD34+ BMSC

were clarified and counted by flow cytometry. Data were

calculated and managed with SPSS 10.0 statistical package.

RESULTS: The amount of the CD34+ stem cells homing to myocardium

in each group were (183.0±9.2)×104/L, (71.1±9.2)×104/L,

(82.3±9.5)×103/L and (78.5±9.8)×103/L, respectively. We

found after comparing the data of each group that the difference

among every group was very significant (P=0.000, respectively)

except between group 3 and 4 (P=0.912). CONCLUSION: GCSF could

not only vastly mobilize the BMSC into periphery blood, but also

could increase the CD34+ stem cells homing to myocardium

independent of myocardial infarct.

【Keywords】 stem cell; myocardial infarction; homing;

granulocyte colonystimulating factor

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【摘要】 目的：观察粒细胞集落刺激因子（GCSF）对 CD34+骨

髓干细胞（BMSC）在心肌内归巢的影响. 方法：成龄杂种犬 17 只，随

机分 4 组. A 组 5 只：接受 10 mg/（kg・d） 5 d 皮下注射和 ，

实验性心梗；B 组 4 只：接受 10 mg/（kg・d） 5 d 皮下注射 ，

和假手术；C 组 4 只：接受 10 mg/（kg・d） 5 d 生理盐水皮 ，

下注射和实验性心梗；D 组 4 只：接受 10 mg/（kg・d） 5 d ，

生理盐水皮下注射和假手术，对每只动物股动脉插管至主动脉根部，

左颈浅静脉插管至冠状静脉窦，分别于造型前后抽血 5 mL（抽复管） ，

裂解红细胞，分离单个核细胞，流式细胞仪测定 CD34+细胞数，数据

分析用 SPSS 10.0 统计软件包. 结果：4 个实验组 CD34+ BMSC 心肌内

归巢数分别为： 183.0±9.2）×104 个/L,（71.1±9.2）×104 个/L, （

（82.3±9.5）×103 个/L 和（78.5±9.8）×103/L，除 C，D 组之间

差别无统计学意义（P=0.912）外，其余各组间均有统计学意义（均为

P=0.000). 结论：GCSF 不仅能动员 BMSC 大量释放入外周血，同时还

能增加 CD34+ BMSC 在心肌内的归巢.

【关键词】 干细胞；心肌梗死；归巢；粒细胞集落刺激因子

0 引言

心肌梗死（myocardial infarction, MI）已成为第一位致死性

疾病，以往认为坏死心肌无法治愈，现在研究发现干细胞能在体内外

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