麝香乌龙丸论文：麝香乌龙丸对AA大鼠滑膜衬里层病理形态及bFGF、TGF-β1表达的影响.doc

麝香乌龙丸论文：麝香乌龙丸对AA大鼠滑膜衬里层病理形态及bFGF、TGF-β1表达的影响

【中文摘要】观察麝香乌龙丸对佐剂关节炎(AA)大鼠滑膜衬里层病理形态及碱性成纤维细胞生长因子(bFGF)、转化生长因子β1(TGF-β1)表达水平的影响,探讨麝香乌龙丸治疗类风湿关节炎(rheumatoid arthritis,RA)是否与调节bFGF和TGF-β1水平有关。方法将健康Wistar大鼠30只,随机分为正常组(A组)、模型组(B组)、麝香乌龙丸组(C组),采用弗氏完全佐剂法建立模型,麝香乌龙丸灌ig1周后,HE染色、光镜下观察各组滑膜衬里层病理变化、衬里层滑膜细胞层数,免疫组织化学法检测各组滑膜衬里层bFGF、TGF-β1表达。结果1滑膜衬里层病理形态观察:A组大鼠滑膜衬里层由1-2层扁平型或低立方形上皮样滑膜细胞组成且部分不连续,滑膜组织无肥厚、充血;B组滑膜衬里层增生到4-8层呈乳头样或栅栏样突起,细胞呈卵圆形,排列紊乱,体积增大,滑膜组织肥厚、充血水肿,形成许多绒毛状突起,突向关节或侵入软骨及骨质;C组衬里层数较B组减少,滑膜细胞轻度增生,滑膜组织充血水肿减轻。2滑膜衬里层细胞层数及bFGF、TGF-β1测定结果比较2.1滑膜衬里层层数B组、C组与A组比较,层数低于A组,差异有统计学意义(P<0.01);C组与B组比较,层数低于B组,差异有统计学意义(P<0.05)。实验结果表明麝香乌龙丸能降低滑膜衬里层细胞层数,减轻滑膜细胞增生。2.2 bFGF表达水平B组、C组与A组比较,bFGF表达高于A组,差异有统计学意义(P<0.01);C组与B组比较,bFGF表达低于B组,差异有统计学意义(P<0.05)。实验结果表明麝香乌龙丸能降低滑膜衬里层bFGF的表达水平。2.3 TGF-β1表达水平B组、C组与A组比较,TGF-β1表达高于A组,差异有统计学意义(P<0.01);C组与B组比较,TGF-β1表达低于B组,差异有统计学意义(P<0.05)。结论实验结果表明,AA大鼠经麝香乌龙丸ig1周后,麝香乌龙丸能明显减轻滑膜组织充血水肿及滑膜细胞增生,降低衬里层细胞层数,同时显著降低衬里层bFGF、TGF-β1的表达水平。提示麝香乌龙丸治疗RA的作用机理可能与减低滑膜衬里层bFGF、TGF-β1的表达水平,抑制滑膜衬里层细胞增生、减少血管翳形成,从而达到保护软骨和骨的作用。
【英文摘要】To observe the influences of Musk—Aconite—Earthworm Pill on pathomorphology and expression of Basic fibroblast growth factor (bFGF) and Transforming growth factorβ1(TGF—β1) in synovial lining layer of rats with adjuvant arthritis(AA),and to explore its therapeutic effectsrats on rheumatoid arthritis (RA).Methods 30 healthy Wistar rats were randomly divided into three groups: normal group(A group), model group(B group), and Musk—Aconite—Earthworm pill group(C group). To establish the rats with adjuvant arthritis model using Complete Freund adjuvant, fills thestomach with Musk—Aconite—Earthworm pill one week. Then, pathomorphological changes of synovial lining layer, synovial lining cell layers and expression of bFGF and TGF-β1 were observed .Results1.The observation of synovial lining layer pathological form: Group A : rats’synovial lining layer building up by 1-2 layer flat type or low set square epithelial appearance of synovial cells and some of discontinuous , synovial organization without hypertrophy and congestion. Group B: hyperplasia of the synovial lining to 4 to 8 layers in sample or the fence papillary layer sample swelled ,and the cells are ovoid, arranging disorderly, increasing in size; Synovial organization is hypertrophy, congestion and oedema, forming many fluff, quilted axon to joints or invades cartilage and bone. The lining layer in group C belows it in group B, synovial cells mild hyperplasia, synovial organization hyperemia edema ease.2. Result comparison of cell layers of Synovial lining layer and bFGF, TGF-β1 determination:2.1 Synovial lining layers in group B and C compared with group A, layers below in group A, a statistically significant difference (P < 0.01); Group C compared with group B, layer below the B group, a statistically significant difference (P < 0.05).Experimental results show that Musk-Aconite-Earthworm pill can reduce cell layers of synovial lining layer, and synovial cell hyperplasia .2.2 bFGF expression level in group B and C compared with group A , bFGF expression above group A ,a statistically significant difference (P < 0.01); Group C and group B compared , the expression of bFGF belows it in group B, a statistically significant difference (P < 0.05); Experimental results show that Musk-Aconite-Earthworm pill can reduce the bFGF expression level of the synovial lining layer .2.3 The expression level of bFGF, TGF-β1in group B and C compared with group A, the expression of bFGF, TGF-β1 aboves in group A, A statistically significant difference (P < 0.01); Group C compared with Group B ,the expression of TGF-β1 belows it in group B , it is a statistically significant difference (P < 0.05).Conclusion(s) Experimental results show that AA rats fills the stomach with Musk-Aconite-Earthworm pill one week, Musk-Aconite-Earthworm pill can significantly reduce hyperemia ,edema and the hyperplasia of synovial membrane cell, reduce lining layer cell layers ,also significantly reduce lining layer expression level of bFGF, TGF-β1 .Clew that the treatment mechanism of Musk-Aconite-Earthworm pill in RA may reduce lining layer expression level of bFGF, TGF-β1 , Inhibit synovial lining layer cell hyperplasia, reduce blood vessels′formation, thus achieves the function to protect of cartilage and bone
【关键词】麝香乌龙丸 佐剂性关节炎 滑膜衬里层病理形态 bFGF TGF-β1
【英文关键词】Musk-Aconite-Earthworm Pill Adjuvant arthritis synovial lining layer pathomorphology bFGF TGF-β1
【目录】麝香乌龙丸对AA大鼠滑膜衬里层病理形态及bFGF、TGF-β1表达的影响

中文摘要

5-7

英文摘要

7-8

英文缩写

9-10

前言

10-13

材料与方法

13-17

结果

17-19

讨论

19-29

结论

29-30

参考文献

30-35

附图

35-37

综述一 中医治疗类风湿关节炎研究概况

37-41

综述二 转化生长因子β1 与类风湿关节炎

41-49

参考文献

45-49

致谢

49-50

个人简历

50