安捷伦7000A气质联用仪介绍 英文版_29页.pdf

July,2008Page 1Agilent GC/MS PortfolioSmallest footprint,single GC columnPower of MRMIndustry leading GC/MS System 5975C with 7890GC7000A GC/MS Triple Quad5975VL with 6850GCJuly,2008Page 2Agilent 7000A GC/MS Triple Quadrupole7000A Width36 cm/14 inchesOnly 2 inches wider than the 5975C MSDJuly,2008Page 3 Generic GC/MS Triple Quad(QQQ)DetectorQuad 1MassAnalysisCollision GasQuad 2 Collision CellIon SourceIonizeQuad 3MassAnalysisCarrier GasElements of a typical triple quadrupole MS systemJuly,2008Page 4Agilent 7000A GC/MS Triple Quad SystemTriple AxisDetectorHeated GoldQuartz Quad1Collision Gas(N2)HexapoleCollision CellIon SourceEI Inert SourceHeated GoldQuartz Quad2The ion source,heated gold plated quartz quadrupoles,and thetriple axis detector are technologies taken from GC/MSDsQ1 Post-filterQ2 Pre-filterCarrier GasJuly,2008Page 5Why GC/MS/MS?A Picture Is Worth a Thousand WordsGC/MS/MS QQQ MRMGC/MS Single Quad SIMEI 100fg HCB in“DIRTY”Matrix S/N:116:1 RMS100 fg HCBMS SIMMS/MS MRMA chromatographersdream:single peak onflat baselineJuly,2008Page 6SIM vs MRM for HCB100 fg HCB in Clean Matrix300 fg HCB in DieselSingle MS:SIM 283.8MS/MS:283.8:213.9S/N=6:1 RMSS/N=86:1RMSS/N=37:1RMSS/N=26:1RMSJuly,2008Page 7TechnologyAgilent took the best technologies from theirindustry leading 597X Series GC/MSD:Heated monolithic gold plated quartz quadrupole Proven reliable high performance source design AUTOTUNEand the 6410A LC/QQQ:Linear acceleration enhanced Collision Cell Wide Mass-Bandwidth QQQ ion optics Mass Hunter softwareJuly,2008Page 8Why Heated Quartz“Gold”Quads?Unlike LC/MS,many high boiling neutral molecules enterthe source and manifold of a GC/MS or GC/MS/MS Higher temperature reduces potential for contamination 200C max Virtually eliminates the need to clean quads Low coefficient for thermal expansion for quartz Stable structure during maintenance cycles(hot-cool-hot)for sourceor detectorMore stable tunes and methods over a longer period of timein real world sample environmentsJuly,2008Page 9GC/QQQ AnalyzerInert,hightemp source(same as 5975)Triple-Axisdetector(sameas 5975)Two gold plated quartz,hyperbolic analyzers(same as 5975)July,2008Page 10GC/QQQ AnalyzerShown with shields foranalyzers and collision cellNew highperformance,hexapolecollision cell withpatent technologyEdwardsSplit Flow TurboJuly,2008Page 11P15-20E-5 TorrP28E-5 TorrRough PumpHe or H2(1-8 sccm)CH4(0-2 sccm)EdwardsSplit Flow TurboCollision CellN2SourceAnalyzerHigh Performance Differentially PumpedManifoldJuly,2008Page 12Analyzer DetailsQuadrupole driveelectronicsHeated Transferlineidentical to GC/MSDJuly,2008Page 13Analyzer Details(hinged side plates)HingesCollision cell cradleJuly,2008Page 14Analyzer Doors OpenDesigned for SupportabilityTriple-AxisDetectorInert,350CSourceJuly,2008Page 15Proven Inert Source PerformanceSeveral thousands sources in current useStay-Clean design extends maintenance intervals withdirty samplesSource tune parameters in the Autotune fileDual filament design reduces maintenance intervalsJuly,2008Page 16EI Source DetailsPartition between sourceand analyzer compartmentsJuly,2008Page 17High Performance Collision Cell DesignLinear acceleration design is optimized for high speedperformance without ion ghosting or cross-talkMRM speed to 500 MRMs/sec allows determination ofmore compounds per ion groupHigh sensitivity with wide mass bandwidth eliminatesthe need to“tune on your compound”for optimumsensitivityPatent pending“Helium Quenching”chemical noisereduction increases S/NJuly,2008Page 18Q1-Collision Cell InterfaceQuadrupolepost-filterJuly,2008Page 19Collision Cell DetailsQuadrupolepost-filterQuadrupolepre-filterSupportcradle(Cell housing and 2 rods removed for clarity)July,2008Page 20High Performance Triple-Axis DetectorUltra low neutrals noiseLong life and high linearity“Gain Normalization”corrects tune file for detector aging toallow repeatable long term method sensitivitySame design as used in the 5975C GC/MSDJuly,2008Page 21Triple-Axis Detectorhyperbolic quartz transmission quadrupole analyzertriple channelelectronmultiplierhigh energydynodesteering rodion beamXYOffsetZshield forsecondaryparticlesJuly,2008Page 22Q2-Detector InterfaceJuly,2008Page 23AutotuneProprietary program tunes the source,mass analyzer,anddetector for(as applicable):Ion transmission Mass axis calibration Mass resolution Detector gain vs VoltageAutotune settings are saved with the method for repeatablemethod performance.Manual Tune override is availableJuly,2008Page 24Agilent 7000A GC/MS/MSOFN(octafluoronaphthalene)Sensitivity272:241 Transition186:1 RMS S/N100fg OFN July,2008Page 25MassHunter MS Workstation SoftwareModern software interpretation of the proven industry standardGC/MS Chemstation platformSingle software platform for all Agilent MS Systems LC/SQ,LC/QQQ,LC/TOF,LC/QTOF GC/SQ,GC/QQQ ICP/MSJuly,2008Page 26GC/MS/MS Software ModulesAcquisition Analysis ExcelReportingJuly,2008Page 27Instrument Control UI/NavigationJuly,2008Page 28MS/MS Acquisition Method Editor DialogSingle window acquisition setupJuly,2008Page 29Agilent 7000A GC/MS Triple Quad FeaturesOutstanding sensitivity 100fg of OFN on column at 100:1 S/N RMS in MS/MSmode using AUTOTUNE parameters verified at customer installation.1050 amu Mass Range500 MRM/sec SpeedReliable heated monolithic gold plated hyperbolic quadrupolesDifferentially pumped vacuum systemNew Helium seeded collision cell technologyAgilent 7890 GC with Capillary Flow technology.MassHunter Software written for GC/QQQ