培养基成分知识.docx

146种培养基成分

1. Acetobacter Medium (醋酸菌培养基) (3~q7Y&B  
Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g +[4FNB  
CaCO3 20g Agar (琼脂) 15g e]UV2&  
Distilled 6r|E:  
适用范围：恶臭醋酸杆菌混浊变种 :MfabpI  
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9}rQ?Btract (牛肉膏) 30g ;]p )Si4  
NaCl 5g Agar (琼脂) 15g k I,w*  
Distilled water (蒸馏水) 7f8N;  
[Note]：When cultivation of Bacillus，5mg of to MnSO4.H2O may be added . s ~.E>BXP  
It is favorable to promote spore formation . E:e%J  
适用范围：产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚种〔青虫菌〕、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、大肠埃希氏菌〔大肠杆菌〕、铜绿假单胞菌〔绿脓杆菌〕、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌 r,\ N\D  
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3. Azotobacter Medium (固氮菌培养基) 8cLC3Fp ;dsCO  
MgSO4.7H2O 0.2g CaSO4.2H2O A8} */$,9w  
Ｎa2MoO4.2H2O Trace(微量) / p${X  
Mannitol(甘露醇) 20g FeCl3 Tract(微量) ^h+mpq2y  
Distilled water (蒸馏水) 1000ml Agar (琼脂) 15g I9tuvcGqng  
;(]Ej8)mM  
适用范围：固氮菌、胶质芽孢杆菌 bVB *uBjN  
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4. Corn Meal Medium (玉米粉培养基) -X.o2X  
Maize flour (玉米粉) 5g Peptone (蛋白胨) IKSbF6^  
Glucose (葡萄糖) 1g Tap water (自来水) 1000ml {3Ug]_mw6f  
[Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小时,分装入18ⅹ18毫米试管,每管深度达6厘米。15磅再次灭菌15小时。) jy5{;/@  
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5. Lactic-bacteria Medium I (乳酸菌培养基 I ) {a[Y L&e  
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Glucose (葡萄糖) 10g KH2PO4 2g _X"=2Z.|j1  
Tomato juice (西红柿汁) 100ml Tween P Arw$Gr  
Distilled water z0l^) 5  
适用范围：植物乳杆菌〔胚芽乳杆菌〕、嗜热乳酸链球菌 zfn2_Pm3#  
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6. Lactic-bacteria Midium Ⅱ (乳酸菌培养基Ⅱ) V?d^< ]  
Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g ,:sq[dl  
Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g ^Hr6i u  
Tween (吐温) 80 1g K2HPO4 2g JWi]D+p  
Na-acetate (醋酸钠) 5g Diamine citrate (柠檬酸二胺) 2g .H.SPIB!w ?2yC;`kU  
Distilled water (蒸馏水) 1000m pH 6.5-6.8 }S9d SiQ  
适用范围：植物乳杆菌〔胚芽乳杆菌〕 qA8BHxO.I  
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7. Peotone Glucose Yeast extract Medium PGY 〔蛋白胨、酵母膏、葡萄糖培养基〕 Z{$9}F-93x  
Peptone〔蛋白胨〕10g Yeast extract 〔酵母膏〕5g }e\nw  
Glucose 〔葡萄糖〕1g Distilled water 〔蒸馏水〕1L Yq:W^_=c  
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8. Glycerol Agar 〔甘油琼脂〕 [VFC x'IO  
Peptone 〔蛋白胨〕5g Beef extract 〔酵母膏〕3g \ # <_zs  
Glycerol 〔甘油〕20g Top water 〔自来水〕 1000ml Oc>}$04%  
Agar " 9'.l(s  
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9. Rhizobium medium 〔根瘤菌培养基〕AS 9 EK/q_h2  
Yeast eztract 〔酵母膏〕1g Soil eztract 〔土壤浸提液〕 200ml ZxASrJp  
Mannitol 〔甘露醇〕10g Agar 〔琼脂〕15g GTmI Q>Pr  
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[Note]：Soil extract：Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法：取土壤50克，加水200毫升，15磅蒸煮1小时，经滤纸过滤后加水补足到200毫升。) W "f-(+8/F  
适用范围：大豆根瘤菌〔慢生型〕、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、 HBBi])q  
大豆根瘤菌〔快生型〕、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌 KTFzg  
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10. Mannitol Agar (甘露醇琼脂) c)VY_r>-  
Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3g c{L"*n>  
Mannitol (甘露醇) 25g Agar (琼脂) 15g 6cc'i  
Distilled water (蒸馏水) 1000ml _ P=FRa7  
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11. Glucose Asparagine 〔葡萄糖、天门冬素琼脂〕 2O [c`Gc^!  
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K2HPO4 0.5g Water 〔水〕 1000ml SUj u]  
pH 7.2-7.4 [d}mm  
适用范围：刺孢小单孢菌绛红变种、紫色小单孢菌〔绛红小单孢菌〕 VYe6:U!k  
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Agar 〔琼脂〕20g water 〔水〕 1000ml ;%IEdSmD  
pH 7.2-7.4 y9l>!~xx  
适用范围：刺孢小单孢菌绛红变种、紫色小单孢菌〔绛红小单孢菌〕、白黄链霉菌、白色链霉菌、抗生链霉菌、双重轮丝链霉菌、产色链霉菌、烬灰链霉菌、天蓝色链霉菌、灭蚊链霉菌、红霉素链霉菌、青色链霉菌、球孢链霉菌、浅灰链霉菌、灰色链霉菌、吸水链霉菌、淡紫灰链霉菌、黄色长孢链霉菌、藤黄色链霉菌、细黄链霉菌、黑化链霉菌、玫瑰色链霉菌、华美链霉菌、嗜热链霉菌、委内瑞拉链霉菌、紫色直丝链霉菌、紫色链霉菌、绿色链霉菌 q.1Zd<(A  
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13. Wort Agar 〔麦芽汁琼脂〕 v`p0<)C  
Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then distribute the medium into tubes. Autoclave at 110 for 30 minutes. (将发酵啤酒的原料〔未加酒花〕，稀释至12柏林，加琼脂15克，溶化后分装。15磅灭菌30分钟。) L.(v红曲霉菌 ]3 P:e}l  
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14. Potato Dextrose Agar PDA (马铃薯、葡萄糖琼脂) U bVvhC  
Potato extract (马铃薯汁) 1000ml Dextrose(glucose) (葡萄糖) 20g Qeb*XWq  
Agar (琼脂) 20g A4tion of juice and boil until soft. (approximately 30min.). Filter through cotton-cloth. Autoclave at 115℃ for 20 minutes.〔注：取去皮马铃薯200克，切成小块，加水1000毫升煮沸30分钟，滤去马铃薯块，将滤液补足至1000毫升，加葡萄糖20克，琼脂15克，溶化后分装，15磅灭菌30分钟。〕 M!FaO Wb  
适用范围：酵米面假单胞菌〔酵米面黄杆菌〕、白色链霉菌、烬灰链霉菌、青色链霉 R:dq/qkh3  
菌、球孢链霉菌、灰色链霉菌、龟裂链霉菌、伞枝梨头霉、雅致放射毛霉、棒曲霉、 iW~G* 霉、棉铃虫拟 ;C|&w:U,f  
青霉、拟青霉、球形阜孢、白腐菌、少根根霉、华根霉、科恩根霉、戴尔根霉、日本根霉、爪哇根霉、米根霉、点头根霉、绿穂霉、簇孢匍柄霉、雅致枝霉、康宁木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢 ?* :<5[U  

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15. Czapek′s Agar〔察氏琼脂〕 Qz+9B&I  
Sucrose 〔蔗糖〕30g NaNO3 3g ^On nv UIV[9uw}T  
FeSO4.7H2O 0.01g K2HPO4 1g '*nsNAH M  
Agar (琼脂) 13g Distilled water (蒸馏水) 1000ml -n~#MTE  
pH 7.2 !o7(5f &  
适用范围：红霉素链霉菌、洋葱曲霉、金头曲霉、泡盛曲霉、泡盛曲霉烟色变种、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、无花果曲霉、黄柄曲霉、黄曲霉、烟曲霉、构巢曲霉、黑曲霉、赭曲霉、米曲霉、寄生曲霉、酱油曲霉、土曲霉、宇佐美曲霉、焦曲霉、杂色曲霉、温特曲霉、阿达青霉、产黄青霉、黄绿青霉、桔青霉、顶青霉、园弧青霉、绳状青霉、灰黄青霉、岛青霉、淡紫青霉、特异青霉、赭绿青霉、产紫青霉、娄地青霉、小刺青霉、荨麻青霉、 j?UQq[k$ K  
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16. Concentrated Sucrose Czapek′s (浓糖察氏琼脂) r? AGZ p6  
Sucrose (蔗糖) 200g NaNO3 3g wF.W$K Xysobmr0X  
FeSO4.4H2O 0.01g K2HPO4 1g f|&!qmM  
Agar (琼脂) 13g Distilled water (蒸馏水) 1000ml Zp#_}  
适用范围：谢瓦曲霉 XJNsz  
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|Pnthetic Potato Medium (综合马铃薯培养基) i>2g3D2GO  
20%Potato extract (马铃薯汁) 1L Glucose (葡萄糖) 20g NcNr;Es?B )12;9/y  
Vitanib B1 (硫胺素) Trace (微量) Agar (琼脂) 15g > (  
pH 6 JTZ(1m  
适用范围：米曲霉、酱油曲霉、栖土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、顶头孢霉、巴西毛客、长刺毛壳、球毛壳、橄榄包毛壳、反曲毛壳、琥珀毛壳、圆酵毛壳、多主枝孢、束状刺盘孢、葫芦科刺盘孢、新月弯孢霉、奇异翅孢壳、地生翅孢壳、串珠镰孢蚀脉镰孢霉、盘长孢菌〔鲁保一号〕、银白杨盘长孢、瓶毛壳、金龟子绿僵菌、生香毛霉、卷枝毛霉、两型孢毛霉、直立毛霉、球孢毛霉、丝球毛霉、大毛霉、多型孢毛霉、总状毛霉、鲁氏毛霉、五通桥毛霉、黑球漆斑菌、露湿漆斑菌、玫烟色拟青霉、棉铃虫拟青霉、淡紫色拟青霉、拟青霉、白腐菌、少根根霉、根霉属的菌、华根霉、科恩根霉、戴尔根霉、台湾根霉、日本根霉、爪哇根霉、黑根霉、雪白根霉、少根根霉、米根霉、点头根霉、三宝垄根霉、根霉属的种、绿穂霉、康宁木霉、木素木霉、拟康氏木霉、木霉、粉红木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢、双孢蘑菇、大肥菇、发光假蜜环菌、木耳、皱木耳、毛木耳、大秃马勃、长根菇、金针菇、棘托竹荪、短裙竹荪、短裙竹荪东北变种〔暂定〕、长裙竹荪、白鬼笔、无裙竹荪、宽鳞大孔菌、凸圆灵芝〔白芝〕、紫芝、灵芝〔红芝，赤芝，日本灵芝，韩国灵芝〕、灰树花、猴头菌、香菇、粗毛斗菇、洁丽香菇、豹皮菇、玉蕈、真姬菇、安络小皮伞菌、鬼毛针、黑柄皮伞菌、尖顶羊肚菌、圆锥羊肚菌、高羊肚菌、黑脉羊肚菌、羊肚菌、滑菇、光帽黄伞、尖鳞黄伞、金顶側耳、榆黄磨、金顶磨、白黄側耳、紫孢平菇、姬菇、裂皮側耳、拟囊体側耳〔鲍鱼菇〕、真线側耳短孢变种、真线側耳、扇形平菇、扁形平菇、佛罗里达无孢子側耳、佛罗里达側耳、蚝菇、糙皮側耳、平菇、贝形側耳、鹰翅菌、肺形側耳、柳树菌、树窝、扁柄側耳、美味側耳、紫孢側耳、粉褶側耳、粉红側耳、凤尾菇、漏斗状側耳、榆干側耳、大榆磨、側耳、茯苓、黑菇、烟色红菇、裂褶菌、鸡纵、金耳、黄木耳、银耳、白木耳、紫晶口磨、银丝菇、丝盖小包脚菇、草菇 Rm{RU  
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18. Corn Meal Medium (玉米粉培养基) %=5]*hu<  
Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into 100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at 121℃ for 30minutes . (20%玉米粉装瓶，15磅灭菌30分钟。) V@ zGy';j  
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19. Filter Paper Medium 〔滤纸培养基〕 ~S H3 D,  
〔NH4〕2SO4 1g KH2PO4 1g F c> ~4k) 96x5!Nj  
Distilled water 〔蒸馏水〕 1000ml A strip of filter paper 〔滤纸条〕 6ⅹ1cm〔厘米〕 \s=|0u  
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20. Soybean Cake Meal Agar (黄豆饼粉琼脂) S"%O_sz  
10% infusion of soybean cake meal (10%黄豆饼粉浸汁) 1000ml Gf q[DF-  
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21. Sawdust， Wheat-bran Medium 〔木屑、麸皮培养基〕 fNo]L*r  
Sawdust of broad leaf tree 75g Wheat bran 25g V(?tVg <|  
Preparation：Mix these two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth . !)_F(~{\{  
壳斗科植物或阔叶树的木屑75克　　麸皮　25克 |# > MT6  
制法：加水适量充分混匀，水量以手捏时指间有水珠为宜，装瓶并压紧，在中心处扎一小洞，堵上棉塞，20磅灭菌1小时。 $;.oyJ  
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22. Pine Block or Pine Sowdust Medium 〔松木条或松木屑培养基〕 N C)5F/O6  
1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (将10ⅹ2ⅹ1厘米的松木条浸泡在1-2%的蔗糖水中。使木条吸足糖水，装瓶不宜过紧，用纸包好，20磅灭菌1小时。) Q"g9}7!  
2. Pine Sawdust Medium we,ci1W  
Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24% (/vm R+U/  
Socrose (蔗糖) 1-2% Water (appropriate)水〔适量〕 x ;A1 a  
Autoclave at 20 lbs for 1 hr.(20磅灭菌1小时〕 4N_w#|RU]  
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23. Rice Straw. Dung Medium (稻草、马粪培养基) ].y)}k!x\I  
Dried horse dung (or cow dung) 〔干马粪或牛粪〕 65% db=. `_y  
Died straw 〔干稻草〕 35% Urea 〔尿素〕 0.5% j18:AnE5`  
Calcium superhosphate 〔过磷酸钙〕0.7% Gypsum powder〔石膏粉〕 1% XS_gW>%s  
Ammonium sulphate 〔硫酸铵〕 0.3% fb6prl$[  
[Note]: Add appropriate water, pile them up and cause them fermentation. 〔水适量进行堆积发酵〕 ~?NUL`t  
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+\jgJPE  
Rj{#,mktJ  
24. Straw Rice Bran Medium 〔稻草、米糠培养基〕 FOappeared between fingers). ( [注]：选无霉稻草，切成长约3厘米的小段，用清水浸泡12小时，使充分吸水，然后倾去水，加米糠和水，水量以用手捏时指间有水珠为宜。) XZF\vb:^'  
R+WzUO]<  
@g(TH!:F  
eFoUvnR  
) 2g of rice into a 15ⅹ150mm tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes. (取大米10克，入50毫升椎形瓶中，水20-30毫升于15磅灭菌30分钟即成，或取大米2克，装入15ⅹ150毫米试管中，加水6毫升，于15磅灭菌30分钟。) xM;(y)LO)  
M'^^cik  
bY[R+ <]  
FUL?x&wR  
g]. @'%=  
26. Filter Paper Medium (滤纸条培养基) VSz6K  
(NH4)2SO4 1g KH2PO4 1g 9bq} !-Y  
MgSO4.7H2O 0.5g Yeast Extract L\rFy8)X  
Distilled Water (蒸馏水) 1000ml M Bu2dQF  
Strip of filter paper 1ⅹ7 cm (滤纸条 1ⅹ7 厘米) C ;j|/n  
[Note]：Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121℃ for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (将上述溶液分配成溶液后分装入试管，每管1毫升，15磅灭菌30分钟。再将灭菌的滤纸条〔1条或2条〕投入试管中即好使用。) tt*FV__D  
\GU~%*7KS:  
3BOMP '[p  
g"b,0x}  
__ f2&tuk  
27. Yeast Extract-Medium Extract Agar I (酵母膏、麦芽膏琼脂) tqo=T1r% X  
Yeast extract (酵母膏) 10g Mall extract (麦芽膏) 10g (Z,,TD  
Glucose (葡萄糖) 4g Distilled water (蒸馏水) 1000ml J b[rh!b  
Agar (琼脂) 20g pH 7.0 &+?8r!  
适用范围：红霉素链霉菌 &2dBo'P:R  
f3 FD-8h>  
F{-iW7R@  
(G3[K L;  
VkQGp)P  
28. Yeast Extract Peptone 〔酵母膏、蛋白胨琼脂〕 BiP g,><  
Yeast extract 〔酵母膏〕1g Multi-peptone〔多蛋白胨〕2g sR)yY}@w  
Beef extract 〔牛肉膏〕1g Glucose 〔葡萄糖〕10g `S$8)]eb&  
Agar 〔琼脂〕20g Distilled water 〔蒸馏水〕1L *T(~(!Vj  
pH 7.0 f l n5wG  
7RJS]f^LO  
\i@warxOr  
K<'gsQVx  
6gS%& 7kW  
29. Glucose Yeast Extract Starch Agar 〔葡萄糖、酵母、淀粉琼脂〕 Z4[L98oX9  
Glucose 〔葡萄糖〕10g Yeast extract 〔酵母膏〕10g :_D#1zd!  
Starch 〔淀粉〕10g NaCl 5g 99}Z;RI  
CaCO3 3g Agar 〔琼脂〕20g Vnj`w"  
R3,YbDt  
Et'#.  
66[(@6M:  
~ovBe]!)  

30. Wheat Bran Medium 〔麸皮培养基〕 #:a xN+(  
Wheat bran 〔麸皮〕36g〔NH4〕2HPO4 10g KkIG D-v  
K2HPO4 0.2g MgSO4。7H2O 6bIw.Nv V  
Agar 〔琼脂〕20g Distilled water 〔蒸馏水〕1L |$FKP  
pH 7.0 ^2}&bDul  
m,?6QlzL0  
kHd> L^2  
cb1  
zKW,f\i'f  
31. Yeast Sporulation Medium (酵母菌产生子囊孢子培养基) yIZG%zU  
(1) Kleyn Medium /y0W8)H  
KH2PO4 0.012% K2HPO4 0.02% ;O&b) )&Q  
Sodium acetate (醋酸钠) 0.5% Glucose (葡萄糖) 0.062% `z]rd,x  
NaCl 0.062% Peptone (蛋白胨) 0.25% d jM=b0  
Biotin (生物素) 2μg% Agar (琼脂) 2% z oGz c  
Trace element solution (混合盐类) 1ml/100ml byQ\Vvu  
[Note]: Trace element solution 2%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2% 9skPx  
(2) Gorodkowa Medium |2\]p!  
Glucose (葡萄糖) 0.1% Peptone (蛋白胨) 1% tI9 ?sug  
NaCl 0.5% Agar (琼脂) 2% 5;4sy*E  
(3) McClary Medium bzpg"3IXA  
Glucose (葡萄糖) 0.1% KCl 0.18% CN ;oO  
Yeast juice (酵母汁) 0.25% Sodium actate (醋酸钠) 0.82% 2fqI B  
Agar (琼脂) 1.5% "f*h-  
7z-{bu#v  
_@$Zrp;%  
-1Z.WOP  
dJwh"y,/  
32. BPY Medium (BPY 培养基) Nj~.GIdd  
Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10g uR!W#x  
NaCl 5g Yeast extract (酵母膏) 5g \@.X`pw 1  
Glucose (葡萄糖) 5g Agar (琼脂) 1-2% _ Hlxt  
Distilled *moW {"r  
适用范围：大肠埃希氏菌〔大肠杆菌〕、恶臭假单胞菌 o$R2d=/)h  
!G>MST  
egWT;2BZ9  
wMc wQ'm  
F$GEH,>d  
33. LB Medium (LB 培养基) v (4mXkf9  
Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10g T5:, :  
NaCl 10g Agar (琼脂) 1-2% 3x{TtoA  
y#q.%  
适用范围：大肠埃希氏菌〔大肠杆菌〕 bx0J*tj0G  
$UvI%; e  
y5Z$WtM  
W8S  
34. Penssay Broth (Penssay 肉汤) )yXwuU 1I  
Beef extract (牛肉膏) 1.5g Yeast 3 B/7 I  
Tryptone ^:#Vx##4f  
Glucose P kry(R  
j,&t23~1Q  
oWEI  n~A  
lmBFn  
fBzAl+GW  
Gk #tW20@  
35. Blond Agar (血琼脂培养基) "2&?z$  
Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g H^ l$y dzAK=48  
Sodium citrate (柠檬酸钠) 5g Brain infusion (脑浸汁) 800ml f)}vti8  
Pig heart infusion (猪心浸) 200ml Sleep Blood (羊血) 10% E L$u4bYZ  
pH 7.2 yO^ dG53t2  
[Note]：Preparation of pig brain and pig heart infusion： Y2xP.A-  
〔1〕 Smash up 100g fresh pig heart 〔without fat and blood vessel〕.Add distilled water up to 1000ml; D5Il"-  
〔2〕 Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml; ~Iq'gN  
〔3〕 Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes; M!XaF~(  
〔4〕 Cool and filtrate through gauze . [注]猪心和猪脑浸汁法：〔1〕新鲜猪心除去心头和脂肪，搅碎100克加蒸馏水至1000毫升；〔2〕新鲜猪脑除去血管，取200克加蒸馏水至1000毫升；〔3〕50℃保温1小时，然后煮开15分钟；〔4〕冷却后用纱布过滤备用。 Hz zMx}!iZ  
C{B[mv7|  
Jpa{>N)xf  
[\O\o|,  
{C H_G  
36. Cellulose﹣decomposing Bacteria Synthetic Medium (纤维细菌合成培养基) Hm#M_Q2 20W@ illed water (蒸馏水) 1000ml YAEw6DP  
xZmM!7R  
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. 〔斜面培养基去滤纸条用2%琼脂及2%羧甲基纤维素钠 CMCNa 〕 tl8a5a i  
QRs*N  
eI Sp  
S=}-J@  
37. Clostridium Pasreurianum Synthetic Medium 〔巴氏梭状芽孢杆菌合成培养基〕 7B>|`?V  
Glucose