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1、ppd 1 1/30.1ml5ppd6-10mm1ppd 2 0.16-10mm7mm9mm8mm 3 4 4872+/2 5mm 59mm+1019mm+20mm+1 2 3 428 1 2 3 4 5 1ppd 1 248 3 4hiv 5 6 7 2ppd 1 2 3 4 5 6-10mm10mm6mm1 7-ppd 1 2 3-4 548-72(6)()bcg vaccine for intradermal injection pinei zhusheyong kajiemiao 3 35iu ppdppd48-725mm 5(0.25mg)/1.1ml1 2.75%1ml(25-26
2、)0.1ml 1.2.3.4.5.2-8 10/1000/()-hbsag-5 g/163 1 2 3 4-10&mug 1.>10 miu/ml 2.5 g-95%85%3.-s19983018 6 86-755-26988688 ():poliomyelitisvaccineindrageecandy(humandiploidcell),live:1g:-20:2:3234-64111:1 2 3:1 237 3:s10870002:1.1/4-2.31230.5ml46 18240.5ml 1.-2.():188781.5-21 295%7-14217-12 1-2 2-3
3、8 4-75%0.5ml 5 ,512(38.3)a615 3 4 a46112233433216433 a28 5 102412 6 1-2 3 10 2412 7 15ml 2 5 3 415-30 8-11234 219322 3a31 4a 5?1.2.1 3.80.5ml270.5ml 2 1.2.3.4.hepatitis a(live)vaccine,freeze-dried ppdgf-nep ppdgf-nep ppdgf-nep taqt4dnadna markertakaradna psimple-19 ecor/baptakarapegfp-1pcdna3.1(+)pc
4、sc-sppw-net 2.1.pdgf dnadnagenbankpdgfgenbankhsn 10c 3,2782bp,ecorf/rbsp106kpndnaf0/r1f1/rpcrpcr1pcr2pcr1pcr2f/rpcr2782bppdgfpcrpsimple-19 ecor/bappsimple-19-pdgf1 f05-cttacgggctgtgtgacctt-3 f5-atcgatagcatggagaggctacaggtagagt-3 f15-tctctgccactgtgccacgct-3 r5-ggtaccggcagtcgatggttcgtcttcactc-3 r15-agc
5、gtggcacagtggcagaga-3 1-pdgf promoter 2.2.nep ncbi genbanknep-actin-actin f 5-acactgtgcccatctacgagggg-3 r 5-atgatggagttgaaggtagtttcgtggat-3 pcsc-sppw-netapaneppegfp-1apaneppegfp-1t4dnabamhkpnnepkpnbamhnepbamhnotpcdna3.1(+)kpnbamhnept4dnakpnnot 2.3.ppdgf-egfp bsp106kpnpsimple-19-pdgfpdgfhindkpnp-egfp-
6、1hind bsp1063ppdgf-egfp f5-agcttcgaattctgcagtcgacggtaccgcgggcccgat r5-cgatcgggcccgcggtaccgtcgactgcagaattcga 2.4.ppdgf-nep kpnnotppdgf-egfppdgfpdgfkpnnotnept4dnappdgf-nep ppdgf-nep-rnatrizoldna markerrakaralipofectaminetm2000invitrogennepsanta cruz-actincell signalingnep-actineclsanta cruz-sk-n-sh293
7、skov3ecvht-29 ppdgf-nep-sk-n-sh293skov3ecvht-29-sk-n-sh-sk-n-sh293skov3ecvht-29 2.2.1rt-pcr trizol(invitrogen)rnarevertaid first strand cdna svnthesis kit20lrna 2lpcr50l2ltaq2.5 u10buffer 5l2 mmol/l dntp mixture 2lnep1l-actinll365l94 3.min9445 s5345 s721 min30-7210 min9l1.5%2.3 26neprt-pcr 1sk-n-sh
8、2sk-n-sh 3293skov3ecvht-29 2.2.2western blot 8090ripabca12sdspage1 h(1/1000)1h4tbsb310min1 htbsb310min 3 36nepwestern blot 1293skov3ecvht-29 2sk-n-sh 3sk-n-sh nepa-mtttakaraelisapromega-a25-35 sigmaa25-35943ul1mm377 a25-354?c20um48h 1.mtt 4sk-n-shppdgf-nep+appdgf-egfp+aask-n-sh5103/9650%60%ppdgf-nep
9、ppdgf-egfpa2535(20mol/l)4810l mtt(5mg/ml)bio-rad570nm63 4 mtt 1sk-n-sh 2ppdgf-nep+a 3ppdgf-egfp+a 4a 2.elisa elisask-n-sh bcg vaccine for intradermal injection /-3 1.35iu5iu ppdppd48725mm 2.1ml 1 3.75G 1ml2526 0.1ml 1.()-t (mcf)-(mif)-mif (maf)-t t 2 4.-2 1.1-2 (1.0cm)(ot)2.3.ot 1.2.()()3.()4.hiv ()
10、5.()6 ()1.2.3.4.2-12 5 6.1.()2.(10mg 2)-(14)-3.-281 yiyanjing pian isoniazid tablets 4-c6h7n3o 137.14 mycolicacid -010%1246t1/20.51.625n-70%2493%63%7%37%-1 2 ppdx 35 hiv 0.3g10mg/kg0.3g5mg/kg0.3g15mg/kg900mg231020mg/kg0.3g30mg/kg500mg-35-1 2-3 4-5b6 1 2-12mg/l,50 1 21 3 4-53 6b6,b6-,-,b6 7(),8alfentanil(disulfiram)-9 10 11 12p-450 13 14-(1)50mg(2)100mg(3)300mg
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