常用载体及通用测序引物复习课程.docx

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3、消费“多样化”Vector

1、荣晓华、孙喜林《消费者行为学》东北财经大学出版社 2003年2月

随着社会经济、文化的飞跃发展，人们正从温饱型步入小康型，崇尚人性和时尚，不断塑造个性和魅力的现代文化价值观念，已成为人们的追求目标。因此，顺应时代的饰品文化显示出强大的发展势头和越来越广的市场，从事饰品销售是有着广阔的市场空间。Primer(F)
Primer(R)
抗性
载体大小(bp)
EZ-T
M13-47
RV-M
Amp+
2984
LZRS-RfA
LZRS-F
LZRS-R

11100
p3xFLAG CMV14
CMV30(834bp)
CMV24(1151bp)

6310
p3XFlag-myc-CMV-24
CMV-F(852bp)/PCEP-F(896bp)
R(hGH polyA)(1217bp)

4752
pAAV-MCS
F(β-globin)(1192bp)
R(hGH polyA)(1521bp)

4650
pAc5.1/V5-His A
Ac5-Profor(2525bp)
BGH(2768bp)/EBVrev(2844bp)
Amp+
5375
pAc5.1/V5-His B
Ac5-Profor(2525bp)
BGH(2772bp)/EBVrev(2848bp)
Amp+
5379
pAc5.1/V5-His C
Ac5-Profor(2525bp)
BGH(2764bp)/EBVrev(2840bp)
Amp+
5371
pAcGP67_A
PH-Profor(4016bp)
pAcGP67rv(4329bp)
Amp+
9761
pAcGP67_B
PH-Profor(4016bp)
pAcGP67rv(4329bp)
Amp+
9761
pAcGP67_C
PH-Profor(4016bp)
pAcGP67rv(4329bp)
Amp+
9761
pACT
T7(1061bp)
T3(1420bp)
Amp+
5566
pACT2
5'AD(同GAL4AD)(5134bp)
3'AD(4939bp)/pGAL4-Adrv(4964bp)(pGAL4-Adrv离MCS少于20bp，优先用3'AD)
Amp+
8117
PACYCDuet-1(MCSⅠ)
DuetUP1(3949bp)
DuetDOWN1(209bp)
Chl+
4008
PACYCDuet-1(MCSⅡ)
DuetUP2(189bp)
T7ter(468bp)
Chl+
4008
pAdTrack
pEGFP-N-3’(同EGFP-Nrev)(1294bp)
CMV-F(1433bp)
Amp+/Kan+
8111
pAdTrack-CMV
pEGFP-N-3’(同EGFP-Nrev)(1272bp)
EBVrev(1982bp)
Kan+
9010
PAO815
5'AOX
3'AOX

7700
pAS2-1
5'BD(同GAL4BD)(5894bp)
3'BD(6112bp)/pGAL4-BDrv(6046bp)(优先用3'BD)
Amp+
8392
pB42AD
pB42ADF(787bp)
pB42ADR(924bp)
Amp+
6449
PBABE-IRES-Egfp
pLXSN-F(同pLXSNfw)(1027bp)/PBABE5'(1063bp)/pBMN5'(1416bp)
无反向引物

5944
pBabe-puro
pBabe5'(1318bp)
pBabe3'(1429bp)

5169
pBACPAK8
BAC1(1181bp)/PH-Profor(1174bp)/BaculoVfor(1211bp)
BAC2(1374bp)/BaculoVrev(1375bp)
Amp+
5538
pBAD/His A
pBADfw(211bp)/Xpressfor(375bp)(优先用pBADfw)
pBADrv(514bp)

4102
pBBR1MCS
M13F(3161bp)/T7(3188bp)/M13-47(3135bp)
M13R(3390bp)/T3(3352bp)/RV-M(3409bp)
Amp+
4707
pBI121
35S(5735bp)
无

14758
pBK-CMV
T7(975bp)/M13F(951bp)
T3(1159bp)/M13R(1196bp)
Amp+
4518
pBluescript II KS (-)
M13F(599bp)/T7(626bp)
T3(792bp)/M13R(829bp)

2961
pBluescript II KS (+)
M13F(599bp)/T7(626bp)
T3(792bp)/M13R(829bp)

2961
pBluescript II SK (-)
M13F(599bp)/T7(626bp)
T3(792bp)/M13R(829bp)

2961
pBlueScript II SK(+)
M13F(599bp)/T7(626bp)
T3(792bp)/M13R(829bp)

2961
pBluescript KS (-)
M13F(602bp)/T7(626bp)
T3(790bp)/M13R(828bp)

2958
pBluescript KS (+)
M13F(602bp)/T7(626bp)
T3(790bp)/M13R(828bp)

2958
pBluescript SK (-)
M13F(602bp)/T7(626bp)
T3(790bp)/M13R(828bp)

2958
pBluescript SK (+)
M13F(602bp)/T7(626bp)
T3(790bp)/M13R(828bp)

2958
pBSN-T
T7
T3
Amp+

pBudCE4.1(MCSI)
CMV-Profor(552)/T7(638)
c-mycrev(742bp)/EBVrev842bp)

4595
pBudCE4.2(MCSⅡ)
PEF-F(2999)
BGH(3235)

4595
pBV220
PBV220F(3592bp)
PBV220R(93bp)

3667
pCAMBIA1300
P1 (11472bp)
P2 (2728bp)

11566
pCAMBIA1301
P1 (10935bp)
P2 (11149bp)

11849
pCAMBIA1304
P1(11454bp)
P2(11668bp)

12361
pCAMBIA2300
M13F(8418bp)/M13R(-48) (8313bp)
M13F(-47)(8461bp)/ M13R(8349bp)

8742
pCANTAB_5E
pCANTAB5-S1(2265bp)/pCANTAB5-R1(2222bp)
pCANTAB5-S6(2471bp)/pCANTAB5-R2(2504bp)

4517
pCAT3-enhancer
RVP3(4215bp)
无
Amp+
4273
pCDFDuet
T7(214bp)
T7ter(467bp)

3781
pCDH-CMV-MCS-EF1-Puro
CMV-F(2164bp)/PCEP-F(2208bp)/pLNCX-F(2210bp)
无

7384
pcDNA1.1
CMV-F(2076bp)/T7(2170bp)
无

4000
pcDNA2.1
M13F/T7
M13R/SP6

3013
pcDNA3.0
pcDNA3fw(841bp)/CMV-Profor(777bp)/T7(863bp)
SP6(1016bp)/BGH(1053bp)

5446
pcDNA3.1 (-)
T7(863bp)/CMV-Profor(777bp)/pcDNA3fw(841bp)
BGH(1039bp)

5428
pcDNA3.1 (+)
T7(863bp)/CMV-Profor(777bp)/pcDNA3fw(841bp)
BGH(1039bp)

5428
pcDNA3.1/His A
T7(863bp)/CMV-Profor(777bp)
BGH(1122bp)
Amp+
5514
pcDNA3.1/His B
T7(863bp)/CMV-Profor(777bp)
BGH(1122bp)
Amp+
5514
pcDNA3.1/His C
T7(863bp)/CMV-Profor(777bp)
BGH(1122bp)
Amp+
5514
pcDNA3.1/myc-His A
T7(863bp)/CMV-Profor777bp)
BGH(1099bp)/c-mycrev(1020bp)
Amp+
5494
pcDNA3.1/myc-His B
T7(863bp)/CMV-Profor(777bp)
BGH(1099bp)/c-mycrev(1020bp)
Amp+
5494
pcDNA3.1/myc-His C
T7(863bp)/CMV-Profor(777bp)
BGH(1099bp)/c-mycrev(1020bp)
Amp+
5494
pcDNA3.1/V5-His A
T7(863bp)/CMV-Profor(777bp)
BGH(1108bp)/V5rev(1035bp)
Amp+
5502
pcDNA3.1/V5-His B
T7(863bp)/CMV-Profor(777bp)
BGH(1108bp)/V5rev(1035bp)
Amp+
5502
pcDNA3.1/V5-His C
T7(863bp)/CMV-Profor(777bp)
BGH(1108bp)/V5rev(1035bp)
Amp+
5502
pcDNA3.1/Zeo(-)
T7(863bp)/CMV-F(769bp)
BGH(1038bp)
Amp+
5014
pcDNA3.1/Zeo(+)
T7(863bp)/CMV-F(769bp)

BGH(1039bp)
Amp+
5015
pcDNA3.3/TOPOTA
CMV-F(584bp)/PCEP-F(628bp)/pLNCX-F(630bp)
无
Amp+
5407
pcDNA4/HisMax A
Xpressfor(1119bp)
BGH(1282bp)
Amp+
5258
pcDNA4/HisMax B
Xpressfor(1119bp)
BGH(1282bp)
Amp+
5258
pcDNA4/HisMax C
Xpressfor(1119bp)
BGH(1282bp)
Amp+
5258
pcDNA4/HisMax-TOPO
Xpressfor(1119bp)
BGH(1302bp)
Amp+
5274
pcDNA4/HisMax-TOPO/lacZ
Xpressfor(1119bp)
BGH(4482bp)
Amp+
8354
pcDNA4/myc-His A
CMV-Profor(777bp)/T7(863bp)
BGH(1103bp)/ c-mycrev(1024bp)
Amp+
5079
pcDNA4/myc-His B
CMV-Profor(777bp)/T7(863bp)
BGH(1103bp)/ c-mycrev(1024bp)
Amp+
5079
pcDNA4/myc-His C
CMV-Profor(777bp)/T7(863bp)
BGH(1103bp)/ c-mycrev(1024bp)
Amp+
5079
pcDNA4/TO
TREfor(908bp)/CMV-Profor(777bp)
BGH(1106bp)
Amp+
5078
pcDNA4/V5-His A
CMV-Profor(777bp)/T7(863bp)
BGH(1117bp)/V5rev(1044bp)
Amp+
5092
pcDNA4/V5-His B
CMV-Profor(777bp)/T7(863bp)
BGH(1117bp)/V5rev(1044bp)
Amp+
5092
pcDNA4/V5-His C
CMV-Profor(777bp)/T7(863bp)
BGH(1117bp)/V5rev(1044bp)
Amp+
5092
pcDNA5/FRT
CMV-Profor(777bp)/T7(863bp)
BGH(1039bp)
Amp+
5069
pcDNA6/myc-His A
CMV-Profor(777bp)/T7(863bp)
BGH(1099bp)/c-mycrev(1020bp)
Amp+
5126
pcDNA6/myc-His B
CMV-Profor(777bp)/T7(863bp)
BGH(1099bp)/c-mycrev(1020bp)
Amp+
5126
pcDNA6/myc-His C
CMV-Profor(777bp)/T7(863bp)
BGH(1099bp)/c-mycrev(1020bp)
Amp+
5126
pcDNA6/V5-His A
CMV-Profor(777bp)/T7(863bp)
BGH(1121bp)/V5rev(1048bp)
Amp+
5148
pcDNA6/V5-His B
CMV-Profor(777bp)/T7(863bp)
BGH(1121bp)/V5rev(1048bp)
Amp+
5148
pcDNA6/V5-His C
CMV-Profor(777bp)/T7(863bp)
BGH(1121bp)/V5rev(1048bp)
Amp+
5148
pcDNAII
T7(2997bp)
SP6(152bp)

3013
pcDNA™6.2-GW/EmGFP-miR
miRNA-F
miRNA-R
Amp+
5699
pCEP4
EBVrev(367bp)
pCEP-F(500bp)
Amp+
10410
pCI(neo)
17Base(996bp)/T7(1067bp)(17Base在T7之前) pCI-neoF886bp)
EBVrev(1206bp)/T3(1158bp)
Amp+
5472
pCMS-EGFP
T7(1067bp)
T3(1158bp)
Amp+
5541
pCMV-3Tag-4A
T3(621bp)/PFLAG-CMV-F(536bp)
T7(906bp)/BGH(952bp)

4233
pCMV5
CMV-F(853bp)/PCEP-F(897bp)
无
Amp+
4657
pCMV5-Flag
pCMV5F
pCMV5R
Amp+

PCMV6
V1.5(815bp)/CMV-Profor(861bp)
XL39(1077bp)/HGHrev(1022bp)
Amp+
4665
PCMV6-XL4
CMV-F(882bp)/T7(952bp)/SP6(952bp)
M13R(1048bp)/HGHrev(1089bp)
Amp+
4707
PCMV-AC
V1.5
XL39(同CMV-24)

5939
pCMV-HA
CMV-Profor(485bp)/TREfor(570bp)
EBVrev(965bp)
Amp+
3782
pCMV-MCS
F(β-globin)
R(hGH polyA)

4492
pCMV-MYC
pCMV-F(794bp)/CMVProfor(490bp)/TREfor(570bp)
pCMV-R(965bp)/EBVrev(973bp)
Amp+
3791
pCMV-Sport1
T7(646bp)
SP6(806bp)/pLNCX-F(924)/CMV-F(970bp)(优先用SP6)
Amp+
4380
pCMV-Sport6
M13F(663bp)/T7(691bp)
SP6(872bp)/M13R(899bp)

4397
pCMV-Tag(KAN+)
T3(621bp)
T7(840bp)
Amp+
4219
pCMV-Tag1(KAN+)
T3(621bp)
T7(840bp)

4219
pCMV-Tag2(KAN+)
T3(621bp)
T7(840bp)

4219
pCMV-Tag-2B
T3(621bp)
T7(845bp)

4323
pCMV-Tag3(KAN+)
T3(621bp)
T7(847bp)

4219
pCMV-Tag-3B
T3(621bp)
T7(846bp)

4300
pCMV-Tag4(KAN+)
T3(621bp)
T7(840bp)

4219
pCMV-Tag5(KAN+)
T3(621bp)
T7(843bp)

4219
PCMX-GAL4(MCS区)
CMV-F
CMV-R

4500
pCOLADuet-1(MCS2)
T7(214bp)/DuetUP2(189bp)(优先用T7)
T7ter(467bp)
Kan+
3719
pcoldⅡ
pcoldI-F(208bp)
pcoldI-R(387bp)

4392
pcoldⅢ
pcoldI-F(208bp)
pcoldI-R(374bp)

4377
pcoldⅣ
pcoldI-F(208bp)
pcoldI-R(356bp)

4377
pcoldI
pcoldI-F(208bp)
pcoldI-R(404bp)

4407
pcoldI
pcoldI-F(208bp)
pcoldI-R(404bp)

4407
pcoldIII
pcoldI-F
pcoldI-R

4751
pCold-SUMO
pCold-SUMO-F
pCold-SUMO-R
Amp+
4751
pCold-TF
pCold-TF-F1
pCold-SUMO-R
Amp+
5769
pCR blunt II TOPO
SP6(239bp)/M13R(205bp)/RV-M(186bp)
T7(425bp)/M13F(449bp)/M13-47(475bp)
Amp+/Kan+

3512
pCR2.1
M13R(205bp)
M13F(406bp，)/T7(382bp)

3907
pCR2.1-TOPO
M13R(205bp)
M13F(409bp)/T7(385bp)(T7离MCS只有6bp，优先用M13F)
Amp+/Kan+
3909
pCR3.1
T7(638bp)
BGH(831bp)
Amp+/Kan+
5060
pCR3.1-Uni
T7(638bp)
BGH(790bp)

5020
pCR4-TOPO
T3(243bp)/M13R(205bp)/RV-M(186bp)
T7(374bp)/M13F(371bp)/M13-47(397bp)
Amp+/Kan+
3956
PCRE-LUC
RVP3(4976bp)
GLP2(327bp)

5034
pCR-ScriptSK
T7/M13F
T3/M13R
Amp+/Kan+

pCS2+ (MCSI)
SP6(35bp)
EBVrev(188bp)
Amp+
4095
pCS2+ (MCSII)
pSG5rv(222bp)
T3(409bp)
Amp+
4095
pDBLEU
pDBLeu-F(6137bp)
pDBLeu-R(6395bp)

9903
pDEST15 Gateway
T7(25bp)/PGEX-F(721bp)/pGEXrv(5270bp)
T7ter(2579bp)
Amp+
7013
PDEST20
PH-Profor(43bp)/PGEX-F(777bp)/EBVrev(2771bp)
无
Amp+
7066
PDEST22
PDEST22-F(4610bp)/pDEST22-F1998(4540bp)
PDEST22-R(6539bp)/pDEST22-R4000(6627bp)
Amp+
8923
pDNR-LIB(MCSA)
M13F(4065bp)/T7(4089bp)
M13R(385bp)

4200
pDNR-LIB(MCSB)
EBVrev(253bp)
M13R(385bp)

4200
pDONR/Zeo
M13F(536bp)
M13R(3044bp)

4291
pDONR201
SeqL-A(286bp)
SeqL-B(2789bp)

4470
pDONR207
SeqL-A(5567bp)
SeqL-B(2485bp)

5585
pDONR221
M13F(536bp)
M13R(3044bp)

4762
pDrive
T7(239bp)/M13R(204bp)
SP6(418bp)/M13F(449bp)
Amp+/Kan+
3851
pDsRED1-C1
pDsRED-ex-C1-F(1222bp)
无

4686
pDSRED1-N1
CMV-Profor(519bp)
RFP-Nrev(751bp)

4692
pDsRED2-C1
pDsRED-ex-C1-F(1219bp)
无反向引物

4675
pDSRED-N1
pEGFP-N-5'
pDSRED-N1-R
Kan+

pEarlygate102
35S(2580bp)

12453
PEarlygate201
35S(2580bp)

11778
pEasy-blunt
M13R(205bp)
M13F(406bp)/T7(382bp)

3929
pEasy-E1
T7(209bp)
T7ter(440bp)(离MCS不到20bp)

5715
pEasy-E2
T7(5117bp)
T7ter(5327bp)

5393
pEasy-T1
M13R(205bp)/RV-M(186bp)
M13F(405bp)/M13-47(431bp)(优先用RV-M和M13-47)

3928
pEasy-T1 Simple
M13R(205bp)/RV-M(186bp)
M13F(306bp)/M13-47(332bp)(优先用RV-M和M13-47)

3829
pEasy-T3
M13F(2996bp)/T7(3036bp删几个碱基后才能找到，最好不用)
M13R(214bp)/SP6(180bp)(优先用M13引物)

3036
pECFP-C1
EGFP-Cfor(1219bp)
SV40-pArev(1576bp)

4731
pECFP-N1
CMV-Profor(524bp)/pEGFP-N-5’(545bp)
pEGFP-N-3’(同EGFP-Nrev)(745bp)

4733
pEF/myc/cyto/ER/mito/nuc
PEF-F(同EF-1aForward)(1133bp)
pCDNA3.1R(1483bp)/c-mycrev(1405bp)

5603
pEF/myc/ER
PEF-F(同EF-1aForward)(1133bp)
BGH(1483bp)
Amp+
5603
pEGFP(3'MCS)
pEGFP-C-5’(942bp)
无反向引物

3355
pEGFP(5'MCS)
M13R(距第一个MCS位点29bp)(205bp)
pEGFP-N-3’(同EGFP-Nrev)(355bp)

3355
pEGFP-C1
EGFP-Cfor(1219bp)/pEGFP-C-5(1267bp，离MCS较近，不建议使用)
SV40-pArev(1575bp)/pEGFP-C-3(1410bp，离MCS较近，不建议使用)

4731
pEGFP-C2
EGFP-Cfor(1219bp)/pEGFP-C-5(1267bp离MCS较近，不建议使用)
SV40-pArev(1579bp)/pEGFP-C-3(1434bp，离MCS较近，不建议使用)

4735
pEGFP-C3
EGFP-Cfor(1239bp)/pEGFP-C-5(1265bp，离MCS较近，不建议使用)
SV40-pArev(1571bp)/pEGFP-C-3(1425bp，离MCS较近，不建议使用)

4727
pEGFP-F
EGFP-Cfor(1219bp)
SV40-pArev(1650bp)

4806
pEGFP-N1
CMV-Profor(519bp)/pEGFP-N-5'(566bp)
pEGFP-N-3’(同EGFP-Nrev)(745bp)

4733
pEGFP-N2
CMV-Profor(524bp)
pEGFP-N-3’(同EGFP-Nrev)(749bp)

4737
pEGFP-N3
CMV-Profor(524bp)
pEGFP-N-3’(同EGFP-Nrev)(741bp)

4729
pENTR/D (-TOPO)
M13F(536bp)/SeqL-A(481bp)
M13R(861bp)/SeqL-B(894bp)
Kan+
2580
pET-*(His)
T7(4010bp)
T7Ter(4191p)
Kan+
4636
pET21a
T7(5117bp)
T7Ter(5357bp)

5443
pET22a(AMP+)
T7
T7Ter

5493
pET22b(AMP+)
T7(5117bp)
T7Ter(5426bp)

5493
pET22c(AMP+)
T7
T7Ter

5493
pET-24(+)
T7(4984bp)
T7Ter(5169bp)

pET-24a(+)
T7
T7Ter

5236
pET-24b(+)
T7
T7Ter

5236
pET-24c(+)
T7
T7Ter

5236
pET-24d(+)
T7
T7Ter

5236
pET28a(+)
T7
T7Ter

5369
pET28b(+)
T7
T7Ter

5369
pET28c(+)
T7
T7Ter

5369
pET-30_Ek/LIC
T7
T7Ter
Amp+
5439
pET-30_Xa/LIC
T7
T7Ter
Amp+
5448
pET-30a(+)
T7(4988bp)
T7ter(5355bp)

5422
pET-30b(+)
T7
T7Ter

5422
pET-30c(+)
T7
T7Ter

pET-32a
S.tag(5631bp)
T7ter(5833bp)

5900
pET-32b
S.tag
T7ter

5900
pET-32c
S.tag
T7ter

5900
pET-33b(+)
T7(4984bp)
T7ter(5316bp)
Kan+
5383
pET-34b(+)
T7
T7ter
Kan+
5918
pET-3a
T7(1bp)
T7ter(186bp)
Amp+
4640
pET-3b
T7(1bp)
T7ter(185bp)
Amp+
4639
pET-3c
T7(1bp)
T7ter(184bp)
Amp+
4638
pET-3d
T7(1bp)
T7ter(183bp)
Amp+
4637
pET-40b(+)
S.tag(5888bp)
T7ter(6123bp)
Kan+
6190
pET-41a(+)
S.tag(5603bp)/PGEX-F(5455bp)
T7ter(5866bp)
Kan+
5933
pET-41b(+)
S.tag(5603bp)/PGEX-F(5455bp)
T7ter(5865bp)
Kan+
5932
pET-41c(+)
S.tag(5603bp)/PGEX-F(5455bp)
T7ter(5867bp)
Kan+
5934
pET-42a(+)
S.tag(5603bp)/PGEX-F(5455bp)
T7ter(5863bp)
Kan+
5930
pET-42b(+)
S.tag(5603bp)/PGEX-F(5455bp)
T7ter(5862bp)
Kan+
5929
pET-42c(+)
S.tag(5603bp)/PGEX-F(5455bp)
T7ter(5864bp)
Kan+
5931
pET-43.1a(+)
无
T7ter(7209bp)

7275
pET-43.1b(+)
无
T7ter(7207bp)

7274
pET-43.1c(+)
无
T7ter(7163bp)

7230
pET-44_Ek/LIC
S.tag/T7(如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
T7ter
Amp+
7331
pET-44a(+)
S.tag(6545bp)/T7(4870bp,如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
T7ter(7244bp)
Amp+
7310
pET-44b(+)
S.tag/T7(如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
T7ter
Amp+
7310
pET-44c(+)
S.tag/T7(如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
T7ter
Amp+
7310
pET-45b(+)
T7(4870bp)
T7ter(5193bp)
Amp+
5260
pET46-Ek/LIC
T7(4870bp)
T7ter(5133bp)
Amp+
5200
pET49b
PGEX-F
T7ter

5926
pET-49b(+)
PGEX-F(5455bp)
T7ter(5859bp)

5926
pET50b(+)
S.tag(6545bp)/T7(4751bp，如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
T7ter(6666bp)

6733
pET-9a
T7(3711bp)
T7ter(3896bp)
Kan+
4341
pET-9b
T7(3711bp)
T7ter(3895bp)
Kan+
4340
pET-9c
T7(3711bp)
T7ter(3894bp)
Kan+
4339
pETDuet-1(MCSI)
pBRrevBam(5297bp)
DuetDOWN1(208bp)
Amp+
5420
pETDuet-1(MCSII)
DuetUP2(189bp)/T7(214bp)
T7ter(467bp)
Amp+
5420
pEYFP-C1
pEGFP-C-5’(1266bp)
pEGFP-C-3'(1429bp)/SV40-pArev(1575bp)
Kan+
4731
pEYFP-N1
pEGFP-N-5’(545bp)/CMV-Profor(519bp)
pEGFP-N-3’(同SV40-pArev)(745bp)
Kan+
4733
pFastBac Dual (MCSI)
BaculoVfor(4531bp)/PH-Profor(4494bp)
FastBac-rev(4753bp)/SV40-pArev(4884bp)

5238
pFastBac Dual (MCSII)
HSVtk-pArev(4236bp)
p10-Profor(4456bp)

5238
pFastBac HT_A
pFastBac-fw(4040bp)/PH-Profor(3920bp)/BaculoVfor(3957bp)
pFastBac-rev(4271bp)

4856
pFastBac HT_B
pFastBac-fw(4040bp)/PH-Profor(3920bp)/BaculoVfor(3957bp)
pFastBac-rev(4271bp)

4856
pFastBac HT_C
pFastBac-fw(4040bp)/PH-Profor(3920bp)/BaculoVfor(3957bp)
pFastBac-rev(4271bp)

4856
pFastBac1
PH-Profor(3920bp)/BaculoVfor(3957bp)
pFastBac-rev(4191bp)

4776
pFastBac1-Gus
无正向引物
SV40-pArev(6208bp)

6661
pFastBacHT-CAT
无正向引物
SV40-pArev(5046bp)

5500
pFLAG-ATS
N26for(18bp)
pTrcHis-rv(同pBADrv)(285bp)

5408
pFLAG-CMV1
pFLAG-CMV-F(863bp)/CMV-30(834bp)
pFLAG-CMV-R(1125bp)/CMV-24(1144bp)
Amp+
4732
pFLAG-CMV2
pFLAG-CMV-F(863bp)/CMV-30(834bp)
pFLAG-CMV-R(1083bp)/CMV-24(1102bp)
Amp+
4679
pFLAG-MAC
N26for(18bp)
pTrcHis-rv(同pBADrv)(225bp)

5063
pGAD424
GAL4AD(5'AD)(757bp)
3'AD(937bp)/pGAL4-Adrv(900bp)
Amp+
6659
pGADGH
GAL4AD(5'AD)(748bp)
T7(933bp)/3'AD(1045bp)
Amp+
8009
pGADT7
5'AD(1827bp)/T7(1905bp)
pGAL4-Adrv(2075bp)

7988
pGADT7