工作汇报-课件.ppt

单击此处编辑母版标题样式,单击此处编辑母版文本样式,第二级,第三级,第四级,第五级,*,*,研究背景,工作进展,小结和工作计划,工作回顾,2,报告内容,C,ontents,Background,Review,Progress,Summary&Plan,1.,研究背景,真菌次生代谢产物结构新颖活性丰富，已成为临床用药重要来源,1,1,J.Antibiot.,2005,58,1,26.,3,抗生素,:,青霉素,降胆固醇：,洛伐他汀,免疫抑制剂,:,环孢菌素,真菌来源广泛，特殊生境中真菌资源丰富,4,1.,研究背景,编号,拉丁名,属名,宿主,采集地,采集时间,J13-2-12Y,Phyllosticta,sp,.,叶点霉,属,石菖蒲,广西药用植物园,2013.12,子囊菌门,(Ascomycota),盘菌亚门,(,Pezizomycotina,),座囊菌纲,(,Dothideomycetes,),葡萄座腔,菌目,(,Botryospaerials,),葡萄座腔,菌科,(,Botryospaeriacea,e,),8,1.,研究背景,叶点霉属,(,Phyllosticta,sp,.,),6,Phytochemistry.,2014,100,103-109.,7,J,ournal of th,e,C,hemical,S,ociety,.,1971,10,1623-1627.,8,BMC Microbiol,ogy.,2014,14,297.,9,A,frican,Jo,urnal of,B,iotechnology.,2011,10,1428-1435.,叶点霉属真菌（,Lasiodiplodia,sp,.,）的次生代谢产物文献报道,含氮类化合物,(,1,0/55,),6-9,7,1.,研究背景,萜类化合物,(,2/55,),2,3-,2,4,2,3,Phytochemistry,.,1994,35,835-839.,2,4,J,ournalof,B,r,a,zilian,Chem,ical,So,ciety,.,2008,19,478-482.,11,1.,研究背景,非芳香类聚酮(,16/55,),1,0,-1,6,聚酮类化合物,(,40/55,),1,0 Tetrahedron Letter,s.,2014,55,453-455.,1,1 Journal of.Bioscience.,2000,55,546-551.,1,2,Tetrahedron Letters.,1983,24,2407-2410.,13 Tetrahedron Letters.,1982,21,1119-1121.,14 ACS Chemical Biology.,2014,9,1119-1127.,15 Phytochemistry.,2000,54,489-494.,16 Natural Product Research.Ahead of Print.,8,1.,研究背景,含,芳香结构的聚酮(,16/55,),1,0,-1,6,聚酮类化合物,(,40/55,),1,0 Tetrahedron Letter,s.,2014,55,453-455.,1,1 Journal of.Bioscience.,2000,55,546-551.,1,2,Tetrahedron Letters.,1983,24,2407-2410.,13 Tetrahedron Letters.,1982,21,1119-1121.,14 ACS Chemical Biology.,2014,9,11191127.,15 Phytochemistry.,2000,54,489-494.,16 Natural Product Research.Ahead of Print.,8,1.,研究背景,其他类,(,22/55,),1,7,-,22,聚酮类化合物,(,40/55,),17 Patent CN103450143B,2014,.,1,8,Phytochemistry,.,2014,103,145-153.,1,9,B,ioscience,B,iotechnology and,B,iochemistry,.,2005,69,1610-1612.,9,1.,研究背景,20,Phytochemistry,.,1987,26,1037-1039.,21,B,ioscience,B,iotechnology and,B,iochemistry.,2008,72,2069-2073.,22,JP63030445A,1988.,10,1.,研究背景,次生代谢产物及其生物活性,化合物编号,5,、,21,抗菌活性,13,、,16,抗炎活性,11,、,14,、,16,抑制骨质疏松,20,、,21,、,22,、,23,、,24,、,33,、,34,诱导马铃薯微茎,5,对链球菌、拟杆菌、消化链球菌显示出抗菌活性,6,；21,对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌的,MIC,值分别为,64,和,128mg/mL,11,。,13、16,均抑制盐皮质激素受体和前列腺素的合,成,13,。,11、14、16,均促进成骨细胞增殖活性,16,。,20、21、22、23、24、33、34,均,诱导马铃薯,微茎,13,14,15,17,。,生物活性,12,1.,研究背景,液相分析,中性条件,208 nm,220 nm,254 nm,280 nm,208 nm,流速,：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),甲醇-水系统,分析条件,：40%,-,70%(0-8 min),70%-90%(8 min-16 min),90%-100%(16 min-26 min),100%-100%(26 min-,45,min),100%,-40,%(,45,min-,46,min,),ELSD,14,液相分析,酸性条件,208 nm,220 nm,254 nm,280 nm,365 nm,流速,：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),甲醇-水系统,分析条件,：40%,-,70%(0-8 min),70%-90%(8 min-16 min),90%-100%(16 min-26 min),100%-100%(26 min-,45,min),100%,-40,%(45,min-,46,min,),ELSD,15,中性与酸性,208 nm,对比图,中性,酸性,流速,：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),甲醇-水系统,分析条件,：40%,-,70%(0-8 min),70%-90%(8 min-16 min),90%-100%(16 min-26 min),100%-100%(26 min-,45,min),100%,-40,%(,45,min-,46,min,),16,化学分离流程（固相萃取）,环己烷洗脱部位,总样,甲醇洗脱部位,检测方法：,ELSD,流速,：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),酸性甲醇-水系统,分析条件,：,40,%,-70,%,(,0,-,8,min,),70,%,-,90,%,(,8,min,-16 min),90%-100%(16 min-26 min),100%-100%(26 min-,45,min),100%,-40,%(,45,min-,46,min,),17,化学分离流程（固相萃取）,环己烷洗脱部位,总样,甲醇洗脱部位,检测波长：,208 nm,流速：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),酸性甲醇-水系统,分析条件：,40%,-,70%(0-8 min),70%-90%(8 min-16 min),90%-100%(16 min-26 min),100%-100%(26 min-,45,min),100%,-40,%(,45,min-,46,min,),18,固相萃取,TLC,分析,环己烷洗脱部位,=,C,总样,=,Z,甲醇洗脱部位,=,W,氯仿：甲醇,=,98:2,19,检测方法：,ELSD,流速：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),酸性甲醇-水系统,分析条件：,40%,-,70%0-8 min),70%-90%(8 min-16 min),90%-100%(16 min-26 min),100%-100%(26 min-,45,min),100%,-40,%(,45,min-,46,min,),化学分离流程（,ODS,拆分）,W,总,W,1,W,2,W,3,W,4,W,5,KB,20,检测波长：,208,nm,流速：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),酸性甲醇-水系统,分析条件：,40%,-,70%(0-8 min),70%-90%(8 min-16 min),90%-100%(16 min-26 min),100%-100%(26 min-,45,min),100%,-40,%(,45,min-,46,min,),化学分离流程（,ODS,拆分）,W,总,W,1,W,2,W,3,W,4,W,5,KB,21,环己烷与,W4,液相,比对,W,4,C,检测方法：,ELSD,流速：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),酸性甲醇-水系统,分析条件：,40%,-,70%(0-8 min),70%-90%(8 min-16 min),90%-100%(16 min-26 min),100%-100%(26 min45,min),100%,-40,%(,45,min-,46,min,),22,检测波长：,208,nm,流速：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),酸性甲醇-水系统,分析条件：,40%,-,70%(0-8 min),70%-90%(8 min-16 min),90%-100%(16 min-26 min),100%-100%(26 min-,45,min),100%,-40,%(,45,min-,46,min,),环己烷层与,W4,液相,比对,W,4,C,23,环己烷层与,W4,合并,TLC,分析,碘熏,香草醛通过,TLC,分析与硅胶柱层析小试，确定展开系统为环己烷,-,乙酸乙酯。初步选取由纯环己烷 到纯乙酸乙酯等,12,个极性段洗脱。,环己烷：乙酸乙酯,=,9:1,24,J13-CW4,(20 g),J13-CW4-2,(2.2 g,11.0%),J13-CW4-1,(6.5 g 32.5%),J13-CW4-3,(3.7 g,18.5%),J13-CW4-4,(3.4 g,17.0%),J13-CW4-5,(0.8 g,4.0%),J13-CW4-6,(1.0 g,5.0%),J13-CW4-7,(2.1 g,10.5%),149:1,98:2,95:5,9:1,1:1,MeOH,过硅胶柱,(20 60 cm),环己烷层与,W4,合并拆分,7:3,硅胶柱层析：上样量,20 g,，,柱体积,V0=1.1 L,。,硅胶柱规格：,=20 60 cm,，体积为,2.5 L,。,流动相系统：环己烷,-,乙酸乙酯。,25,J13-CW4,液相分析,J13-CW4-3,J13-CW4,检测方法：,ELSD,流速：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),酸性甲醇-水系统,分析条件：,70%(0-,2,min),70%-,85,%(,2,min-,20,min),85,%-,97,%(,20,min-26 min),97,100%(26 min-,42,min),100%,-100,%(,42,min-,52,min,),J13-CW4-4,J13-CW4-6,J13-CW4-1,J13-CW4-7,J13-CW4-5,J13-CW4-2,KB,26,J13-CW4,液相分析,J13-CW4-3,J13-CW4,检测波长：,208 nm,流速：,0.8,ml/min Phenomenex Gemini 5 C18 column(5 m,4.6250 mm),酸性甲醇-水系统,分析条件：,70%(0-,2,min),70%-,85,%(,2,min-,20,min),85,%-,97,%(,20,min-26 min),97,%-100%(26 min-,42,min),100%,-100,%(,42,min-,52,min,),J13-CW4-4,J13-CW4-6,J13-CW4-1,J13-CW4-7,J13-CW4-5,J13-CW4-2,KB,27,Thank you!,