通用引物和常用引物.doc

通用引物、常用引物及序列对照
General Primers
BGHrev
TAGAAGGCACAGTCGAGG
GFPfusionREV
CTCCACTGACAGAAAATTTG
M13 (-21) Forward
TGTAAAACGACGGCCAGT
M13 (-40)
GTTTTCCCAGTCACGAC
M13 Reverse
CAGGAAACAGCTATGAC
MSCV-rev
CAGCGGGGCTGCTAAAGCGCATGC
pBluescriptSK
TCTAGAACTAGTGGATC
pBluescriptKS
TCGAGGTCGACGGTATC
pGEX3'
CCGGGAGCTGCATGTGTCAGAGG
pGEX5'
GGGCTGGCAAGCCACGTTTGGTG
SP6
ATTTAGGTGACACTATAG
T7 Terminal
GCTAGTTATTGCTCAGCGG
Universal T3
GCAATTAACCCTCACTAAAGG
Universal T7
TAATACGACTCACTATAGGG

Primers from Invitrogen
3'AOX1
GCAAATGGCATTCTGACATCC
5'AOX1
GACTGGTCCAATTGACAAGC
AC5
ACACAAAGCCGCTCCATCAG
AUG1 Forward
CAATTTACATCTTTATTTATTAACG
AUG1 Reverse
GAAGAGAAAAACATTAGTTGGC
BGH Reverse
TAGAAGGCACAGTCGAGG
Baculovirus Forward
TTTACTGTTTTCGTAACAGTTTTG
CMV Forward
CGCAAATGGGCGGTAGGCGTG
CYC1
GCGTGAATGTAAGCGTGAC
EBV Reverse
GTGGTTTGTCCAAACTCATC
EF-1a Forward
TCAAGCCTCAGACAGTGGTTC
Ecdysone Forward
CTCTGAATACTTTCAACAAGTTAC
GAL1
AATATACCTCTATACTTTAACGTC
M13/pUC Forward
CCCAGTCACGACGTTGTAAAACG
M13/pUC Reverse
AGCGGATAACAATTTCACACAGG
Metallothionein
CATCTCAGTGCAACTAAA
OpIE2 Forward
CGCAACGATCTGGTAAACAC
V5 Reverse
ACCGAGGAGAGGGTTAGGAT
Xpress Forward
TATGGCTAGCATGACTGGT
alpha-factor
TACTATTGCCAGCATTGCTGC
cI Forward
GGATAGCCGGTCAGGTGTT
pBAD Forward
ATGCCATAGCATTTTTATCC
pBAD Reverse
GATTTAATCTGTATCAGG
pCEP Forward
AGAGCTCGTTTAGTGAACCG
pGAP
GTCCCTATTTCAATCAATTGAA
pGENE Forward
CTGCTATTCTGCTCAACCT
pHybLex Forward
AGGGCTGGCGGTTGGGGTTATTCGC
pHybLex Reverse
GAGTCACTTTAAAATTTGTATACAC
pJG4-5 Forward
GATGCCTCCTACCCTTATGATGTGCC
pJG4-5 Reverse
GGAGACTTGACCAAACCTCTGGCG
pREP Forward
GCTCGATACAATAAACGCC
pRH Forward
CTGTCTCTATACTCCCCTATAG
pRH Reverse
CAAAATTCAATAGTTACTATCGC
pTrcHis Forward
GAGGTATATATTAATGTATCG
pTrcHis Reverse
GATTTAATCTGTATCAGG
pUni Forward
CTATCAACAGGTTGAACTG
pUni Reverse
CAGTCGAGGCTGATAGCGAGCT
pYESTrp Forward
GATGTTAACGATACCAGCC
pYESTrp Reverse
GCGTGAATGTAAGCGTGAC
Polyhedrin forward
AAATGATAACCATCTCGC
Polyhedrin reverse
GTCCAAGTTTCCCTG

Primers from BD Biosciences
3'AOX1
GCAAATGGCATTCTGACATCC
EGFP-C
CATGGTCCTGCTGGAGTTCGTG
EGFP-N
CGTCGCCGTCCAGCTCGACCAG
GAL1
AATATACCTCTATACTTTAACGTC
Gal4-AD
AATACCACTACAATGGAT
HA
ATGTACCCATACCATGTTCCAGATTAC
HAT
GAGGAGCACGCTCATGCCCAC
LNCX
AGCTCGTTTAGTGAACCGTCAGATC
MSCV
CCCTTGAACCTCCTCGTTCGACC
Myc
GCATCAATGCAGAAGCTGATCTCA
DsRed1-N
GTACTGGAACTGGGGGGACAG
DsRed1-C
AGCTGGACATCACCTCCCACAACG
pCMV
GATCCGGTACTAGAGGAACTGAAAAAC

Other Primers
Bglob-intron-F
CTGGTCATCATCCTGCCTTT
Bglob-pA-R
TTTTGGCAGAGGGAAAAAGA
EXFP-R
GTCTTGTAGTTGCCGTCGTC (Golenbock lab)
GFP-F
GGTCCTTCTTGAGTTTGTAAC
GFP-R
CCATCTAATTCAACAAGAATTGGGACAAC (Ahmad lab)
Gal10pro-F
GGTGGTAATGCCATGTAATATG (Stratagene)
Gal4 N-term
GAGTAGTAACAAAGGTCAA
GPDpro-F
CGGTAGGTATTGATTGTAATTCTG
H1
TCGCTATGTGTTCTGGGAAA
HA-F
TACCCATACGACGTCCCAGA
HA-R
TCTGGGACGTCGTATGGGTA
hGH-PA-R
CCAGCTTGGTTCCCAATAGA
IRES-R
CCTCACATTGCCAAAAGACG
L4440
AGCGAGTCAGTGAGCGAG (Caldwell lab)
LacZ-R
GACAGTATCGGCCTCAGGAA
LexA
CGTCAGCAGAGCTTCACCATTG (Caldwell lab)
LKO.1 5'
GACTATCATATGCTTACCGT (Weinberg Lab)
LucNrev
CCTTATGCAGTTGCTCTCC
MBP-F
GATGAAGCCCTGAAAGACGCGCAG (Waugh lab)
mPGK-F
CATTCTGCACGCTTCAAAAG
MT1-F
GCTGTCCTCTAAGCGTCACC
mU6-F
CAGCACAAAAGGAAACTCACC
NOS-F
GCGTTCAAAAGTCGCCTAAG
pACLU
AGAGCCTGATAAAAACGGTTAGCG (Joung lab)
pBABE 3'
ACCCTAACTGACACACATTCC (Weinberg Lab)
pBABE 5'
CTTTATCCAGCCCTCAC (Weinberg Lab)
pBAC
CGCCAGGGTTTTCCCAGTCACGAC (Joung lab)
pBMN 5'
GCTTGGATACACGCCGC
pBRforBam
CTTGGAGCCACTATCGAC
pBRrevBam
GGTGATGTCGGCGATATAGG
pCasper-F
GGGTTTTATTAACTTACAT (Vosshall lab)
pCAG-F
GCAACGTGCTGGTTATTGTG
pcDL-F
GTTGCCTTTACTTCTAGGCCT (Kinet lab)
pENTR-F
CTACAAACTCTTCCTGTTAGTTAG
pGP
GGGGGATTTCTGTTCATGGGGG (Joung lab)
pGP704-R
AACAAGCCAGGGATGTAACG
pGV-2U
ACTAACATACGCTCTCCATCAA (Yamanaka lab)
pLTet-F
ACTGAGCACATCAGCAGGAC
pLXSN 5'
CCCTTGAACCTCCTCGTTCGACC (MSCV)
pMT2-F
TTGCCTTTCTCTCCACAGGT
pMX-S1811
GACGGCATCGCAGCTTGGATACAC (Yamanaka lab)
puro-F
GCAACCTCCCTTCTACGAGC
RCAS-F
ACATGGGTGGTGGTATAGCGCTTGCG (Orsulic lab)
RVprimer3
CTAGCAAAATAGGCTGTCCC (Promega)
SFFV-F
ATTGATTGACTGCCCACCTC
SV40pA-R
GAAATTTGTGATGCTATTGC
SV40pro-F
TATTTATGCAGAGGCCGAGG
sv40-spliceR
CACAAAGATCCGGACCAAAG
tac promoter
GAGCGGATAACAATTTCACACAGG (Waugh lab)
TRC-F
CAAGGCTGTTAGAGAGATAATTGGA (Root lab)
Ubx-F
AACTCGTACTTTGAACAGGC
WPRE-R
CATAGCGTAAAAGGAGCAACA