2D核磁共振谱2D nuclear magnetic resonance spectrum2.pdf

LR-COSYhttp:/www.xy- 021-676323004.3.2 异核化学位移相关谱 HETCOR(Heteronuclear chemical shift correlation,1H-13C COSY)1.C,H-COSY1.C,H-COSY1.C,H-COSY1.C,H-COSY谱（氢碳化学位移相关谱）谱（氢碳化学位移相关谱）2.COLOC2.COLOC2.COLOC2.COLOC（远程氢碳化学位移相关谱远程氢碳化学位移相关谱)3.3.1 1H H检测的异核化学位移相关谱检测的异核化学位移相关谱 (inverse(inverse实验实验）http:/www.xy- 021-676323001.C,H-COSY谱（氢碳化学位移相关谱）F F F F2 2 2 2域是宽带去偶的碳域是宽带去偶的碳13131313C C C C谱，谱，F F F F1 1 1 1域是氢谱域是氢谱 谱图没有对角峰谱图没有对角峰 季碳不出交叉峰季碳不出交叉峰http:/www.xy- 021-67632300 基本脉冲序列基本脉冲序列基本脉冲序列基本脉冲序列 :13C1Ht112The standard pulse sequence for The standard pulse sequence for The standard pulse sequence for The standard pulse sequence for The standard pulse sequence for The standard pulse sequence for The standard pulse sequence for The standard pulse sequence for 1313131313131313C-detectedC-detectedC-detectedC-detectedC-detectedC-detectedC-detectedC-detected1 1 1 1 1 1 1 1H-H-H-H-H-H-H-H-1313131313131313C chemical shift correlation.C chemical shift correlation.C chemical shift correlation.C chemical shift correlation.C chemical shift correlation.C chemical shift correlation.C chemical shift correlation.C chemical shift correlation.AQAQAQAQhttp:/www.xy- 021-67632300化合物6的二维C,H-COSY谱 http:/www.xy- 021-676323002.COLOC（远程氢碳化学位移相关谱)在在C,H-COSYC,H-COSYC,H-COSYC,H-COSY谱中季碳原子不出现相关峰谱中季碳原子不出现相关峰 确定分子骨架和推断结构很重要确定分子骨架和推断结构很重要 COLOCCOLOCCOLOCCOLOC谱图形式类似于谱图形式类似于C,H-COSYC,H-COSYC,H-COSYC,H-COSY谱谱 http:/www.xy- 021-676323003.1H检测的异核化学位移相关谱 C,H-COSYC,H-COSYC,H-COSYC,H-COSY和和COLOCCOLOCCOLOCCOLOC谱是对谱是对13131313C C C C采样的采样的,因因13131313C C C C核核灵敏度比灵敏度比1 1 1 1H H H H核低得多，测试样品多，累加时间长。核低得多，测试样品多，累加时间长。把检测把检测13131313C C C C信号变为检测信号变为检测1 1 1 1H H H H信号，将大大提高相关信号，将大大提高相关谱的灵敏度。谱的灵敏度。1 1 1 1H H H H检测的异核化学位移相关谱实验检测的异核化学位移相关谱实验称为反转实验（称为反转实验（inverseinverseinverseinverse实验）。包括：实验）。包括：HMQCHMQCHMQCHMQC（1 1 1 1H H H H检测的异核多量子相干实验）检测的异核多量子相干实验）HSQCHSQCHSQCHSQC（1 1 1 1H H H H检测的异核单量子相干实验）检测的异核单量子相干实验）HMBCHMBCHMBCHMBC（1 1 1 1H H H H检测的异核多键相干实验）检测的异核多键相干实验）http:/www.xy- 021-67632300（1）HMQC(Heteronuclear Multiple Quantum Coherence)薄荷醇的HMQC谱 OH23456789101http:/www.xy- 021-67632300C1 C2 C3C4 H1H2H3H4（2）HSQC(Heteronuclear Single Quantum Coherence)HSQC HSQC HSQC HSQC HSQC HSQC HSQC HSQC 与与与与HMQCHMQCHMQCHMQCHMQCHMQCHMQCHMQC的谱图相同，的谱图相同，的谱图相同，的谱图相同，都是都是都是都是显示显示显示显示1 1 1 1 1 1 1 1H H H HH H H H核和与其直接相连的核和与其直接相连的核和与其直接相连的核和与其直接相连的1313131313131313C C C C C C C C核的相关峰，核的相关峰，核的相关峰，核的相关峰，其作用相应于其作用相应于其作用相应于其作用相应于C,H-COSYC,H-COSYC,H-COSYC,H-COSYC,H-COSYC,H-COSYC,H-COSYC,H-COSY谱谱谱谱 http:/www.xy- 021-67632300HSQC 与HMQC的比较 HSQC谱的F1域的分辨率比HMQC的高 HSQC谱的不足之处是脉冲序列比HMQC复杂 http:/www.xy- 021-67632300ppmppmppmppm1.01.01.01.01.51.51.51.52.02.02.02.02.52.52.52.53.03.03.03.03.53.53.53.5ppmppmppmppm101010101515151520202020252525253030303035353535404040404545454550505050555555556060606065656565Current Data ParametersCurrent Data ParametersCurrent Data ParametersCurrent Data ParametersNAME buthsqcNAME buthsqcNAME buthsqcNAME buthsqcEXPNO 1EXPNO 1EXPNO 1EXPNO 1PROCNO 1PROCNO 1PROCNO 1PROCNO 1F2-Acquisition ParametersF2-Acquisition ParametersF2-Acquisition ParametersF2-Acquisition ParametersDate_ 20001102Date_ 20001102Date_ 20001102Date_ 20001102Time 10.31Time 10.31Time 10.31Time 10.31INSTRUM DRX500INSTRUM DRX500INSTRUM DRX500INSTRUM DRX500PROBHD 5 mm TBI 1H/PROBHD 5 mm TBI 1H/PROBHD 5 mm TBI 1H/PROBHD 5 mm TBI 1H/PULPROG invietgpsiPULPROG invietgpsiPULPROG invietgpsiPULPROG invietgpsiTD 1024TD 1024TD 1024TD 1024SOLVENT CDCl3SOLVENT CDCl3SOLVENT CDCl3SOLVENT CDCl3NS 1NS 1NS 1NS 1DS 16DS 16DS 16DS 16SWH 2185.315 HzSWH 2185.315 HzSWH 2185.315 HzSWH 2185.315 HzFIDRES 2.134096 HzFIDRES 2.134096 HzFIDRES 2.134096 HzFIDRES 2.134096 HzAQ 0.2345700 secAQ 0.2345700 secAQ 0.2345700 secAQ 0.2345700 secRG 2298.8RG 2298.8RG 2298.8RG 2298.8DW 228.800 usecDW 228.800 usecDW 228.800 usecDW 228.800 usecDE 6.00 usecDE 6.00 usecDE 6.00 usecDE 6.00 usecTE 300.0 KTE 300.0 KTE 300.0 KTE 300.0 KD0 0.00000300 secD0 0.00000300 secD0 0.00000300 secD0 0.00000300 secD1 2.00000000 secD1 2.00000000 secD1 2.00000000 secD1 2.00000000 secD4 0.00170000 secD4 0.00170000 secD4 0.00170000 secD4 0.00170000 secD11 0.03000000 secD11 0.03000000 secD11 0.03000000 secD11 0.03000000 secD13 0.00000300 secD13 0.00000300 secD13 0.00000300 secD13 0.00000300 secD16 0.00010000 secD16 0.00010000 secD16 0.00010000 secD16 0.00010000 secD24 0.00090000 secD24 0.00090000 secD24 0.00090000 secD24 0.00090000 secDELTA 0.00116720 secDELTA 0.00116720 secDELTA 0.00116720 secDELTA 0.00116720 secDELTA1 0.00110700 secDELTA1 0.00110700 secDELTA1 0.00110700 secDELTA1 0.00110700 secIN0 0.00003600 secIN0 0.00003600 secIN0 0.00003600 secIN0 0.00003600 secl3 256l3 256l3 256l3 256=CHANNEL f1=CHANNEL f1=CHANNEL f1=CHANNEL f1=NUC1 1HNUC1 1HNUC1 1HNUC1 1HP1 5.60 usecP1 5.60 usecP1 5.60 usecP1 5.60 usecP2 11.20 usecP2 11.20 usecP2 11.20 usecP2 11.20 usecP28 2500.00 usecP28 2500.00 usecP28 2500.00 usecP28 2500.00 usecPL1 -4.00 dBPL1 -4.00 dBPL1 -4.00 dBPL1 -4.00 dBSFO1 500.1310815 MHzSFO1 500.1310815 MHzSFO1 500.1310815 MHzSFO1 500.1310815 MHz=CHANNEL f2=CHANNEL f2=CHANNEL f2=CHANNEL f2=CPDPRG2 garpCPDPRG2 garpCPDPRG2 garpCPDPRG2 garpNUC2 13CNUC2 13CNUC2 13CNUC2 13CP3 17.70 usecP3 17.70 usecP3 17.70 usecP3 17.70 usecP4 35.40 usecP4 35.40 usecP4 35.40 usecP4 35.40 usecPCPD2 89.00 usecPCPD2 89.00 usecPCPD2 89.00 usecPCPD2 89.00 usecPL2 -1.00 dBPL2 -1.00 dBPL2 -1.00 dBPL2 -1.00 dBPL12 13.00 dBPL12 13.00 dBPL12 13.00 dBPL12 13.00 dBSFO2 125.7633722 MHzSFO2 125.7633722 MHzSFO2 125.7633722 MHzSFO2 125.7633722 MHz=GRADIENT CHANNEL=GRADIENT CHANNEL=GRADIENT CHANNEL=GRADIENT CHANNEL=GPNAM1 sine.100GPNAM1 sine.100GPNAM1 sine.100GPNAM1 sine.100GPNAM2 sine.100GPNAM2 sine.100GPNAM2 sine.100GPNAM2 sine.100GPX1 0.00%GPX1 0.00%GPX1 0.00%GPX1 0.00%GPX2 0.00%GPX2 0.00%GPX2 0.00%GPX2 0.00%GPY1 0.00%GPY1 0.00%GPY1 0.00%GPY1 0.00%GPY2 0.00%GPY2 0.00%GPY2 0.00%GPY2 0.00%GPZ1 80.00%GPZ1 80.00%GPZ1 80.00%GPZ1 80.00%GPZ2 20.10%GPZ2 20.10%GPZ2 20.10%GPZ2 20.10%P16 1000.00 usecP16 1000.00 usecP16 1000.00 usecP16 1000.00 usecF1-Acquisition parametersF1-Acquisition parametersF1-Acquisition parametersF1-Acquisition parametersND0 2ND0 2ND0 2ND0 2TD 512TD 512TD 512TD 512SFO1 125.7634 MHzSFO1 125.7634 MHzSFO1 125.7634 MHzSFO1 125.7634 MHzFIDRES 27.126736 HzFIDRES 27.126736 HzFIDRES 27.126736 HzFIDRES 27.126736 HzSW 110.437 ppmSW 110.437 ppmSW 110.437 ppmSW 110.437 ppmFnMODE undefinedFnMODE undefinedFnMODE undefinedFnMODE undefinedF2-Processing parametersF2-Processing parametersF2-Processing parametersF2-Processing parametersSI 1024SI 1024SI 1024SI 1024SF 500.1300144 MHzSF 500.1300144 MHzSF 500.1300144 MHzSF 500.1300144 MHzWDW SINEWDW SINEWDW SINEWDW SINESSB 2SSB 2SSB 2SSB 2LB 0.00 HzLB 0.00 HzLB 0.00 HzLB 0.00 HzGB 0GB 0GB 0GB 0PC 0.20PC 0.20PC 0.20PC 0.20F1-Processing parametersF1-Processing parametersF1-Processing parametersF1-Processing parametersSI 1024SI 1024SI 1024SI 1024MC2 echo-antiechoMC2 echo-antiechoMC2 echo-antiechoMC2 echo-antiechoSF 125.7577969 MHzSF 125.7577969 MHzSF 125.7577969 MHzSF 125.7577969 MHzWDW SINEWDW SINEWDW SINEWDW SINESSB 2SSB 2SSB 2SSB 2LB 0.00 HzLB 0.00 HzLB 0.00 HzLB 0.00 HzGB 0GB 0GB 0GB 0H H H HO O O OC C C CH H H H1 1 1 1H H H HC C C CC C C CC C C CH H H H2 2 2 2H H H HH H H H3 3 3 3H H H HH H H H4 4 4 4H H H HH H H H1 1 1 1 1 1 1 12 2 2 2 2 2 2 23 3 3 3 3 3 3 34 4 4 4 4 4 4 41 1 1 1 1 1 1 12 2 2 2 2 2 2 23 3 3 3 3 3 3 34 4 4 4 4 4 4 4Sensitivity Enhanced Gradient HSQCSensitivity Enhanced Gradient HSQCSensitivity Enhanced Gradient HSQCSensitivity Enhanced Gradient HSQCSensitivity Enhanced Gradient HSQCSensitivity Enhanced Gradient HSQCSensitivity Enhanced Gradient HSQCSensitivity Enhanced Gradient HSQChttp:/www.xy- 021-67632300http:/www.xy- 021-6763230013C1Ht1AQC C C C2 2 2 2,C,C,C,C3 3 3 3 and C and C and C and C4 4 4 4:Quaternary or protonated carbons X:O,N:Quaternary or protonated carbons X:O,N:Quaternary or protonated carbons X:O,N:Quaternary or protonated carbons X:O,NC C C C1 1 1 1C C C C2 2 2 2C C C C3 3 3 3C C C C4 4 4 4HHHH1 1 1 1C C C C1 1 1 1X X X XC C C C2 2 2 2C C C C3 3 3 3HHHH1 1 1 1Pulse sequence for HMBCPulse sequence for HMBCPulse sequence for HMBCPulse sequence for HMBCPulse sequence for HMBCPulse sequence for HMBCPulse sequence for HMBCPulse sequence for HMBC用于季碳或自旋系统之间连接点的归属，用于季碳或自旋系统之间连接点的归属，用于季碳或自旋系统之间连接点的归属，用于季碳或自旋系统之间连接点的归属，对应于对应于对应于对应于COLOCCOLOCCOLOCCOLOCCOLOCCOLOCCOLOCCOLOC谱谱谱谱（3）HMBC(Heteronuclear Multiple-Bond Correlation http:/www.xy- 021-67632300HMBC 与HMQC的区别 HMQCHMQCHMQCHMQC是通过异核多量子相干实验把是通过异核多量子相干实验把1 1 1 1H H H H核核和与其直接相连的和与其直接相连的13131313C C C C核关联起来。核关联起来。HMBCHMBCHMBCHMBC则是通过异核多量子相干实验则是通过异核多量子相干实验把把1 1 1 1H H H H核和远程偶合的核和远程偶合的13131313C C C C核关联了起来，其作用核关联了起来，其作用类似于类似于COLOCCOLOCCOLOCCOLOC谱谱 C1 C2 C3C4 H1H2H3H4C C C C1 1 1 1C C C C2 2 2 2C C C C3 3 3 3C C C C4 4 4 4HHHH1 1 1 1http:/www.xy- 021-67632300化合物9的HMBC谱 NOO12345678910109 9 9 9http:/www.xy- 021-67632300化合物10的HMBC谱 OHHOHO1234567891011121314151617181920212223242529302627281 1 1 10 0 0 0http:/www.xy- 021-67632300ppmppmppmppm3.53.53.53.54.04.04.04.04.54.54.54.55.05.05.05.05.55.55.55.56.06.06.06.06.56.56.56.57.07.07.07.07.57.57.57.58.08.08.08.0ppmppmppmppm30303030404040405050505060606060707070708080808090909090100100100100110110110110120120120120130130130130140140140140150150150150160160160160Current Data ParametersCurrent Data ParametersCurrent Data ParametersCurrent Data ParametersNAME cfyhmbcNAME cfyhmbcNAME cfyhmbcNAME cfyhmbcEXPNO 1EXPNO 1EXPNO 1EXPNO 1PROCNO 1PROCNO 1PROCNO 1PROCNO 1F2-Acquisition ParametersF2-Acquisition ParametersF2-Acquisition ParametersF2-Acquisition ParametersDate_ 20001103Date_ 20001103Date_ 20001103Date_ 20001103Time 7.36Time 7.36Time 7.36Time 7.36INSTRUM DRX500INSTRUM DRX500INSTRUM DRX500INSTRUM DRX500PROBHD 5 mm TBI 1H/PROBHD 5 mm TBI 1H/PROBHD 5 mm TBI 1H/PROBHD 5 mm TBI 1H/PULPROG inv4gplrndPULPROG inv4gplrndPULPROG inv4gplrndPULPROG inv4gplrndTD 1024TD 1024TD 1024TD 1024SOLVENT CDCl3SOLVENT CDCl3SOLVENT CDCl3SOLVENT CDCl3NS 4NS 4NS 4NS 4DS 16DS 16DS 16DS 16SWH 3443.526 HzSWH 3443.526 HzSWH 3443.526 HzSWH 3443.526 HzFIDRES 3.362818 HzFIDRES 3.362818 HzFIDRES 3.362818 HzFIDRES 3.362818 HzAQ 0.1488800 secAQ 0.1488800 secAQ 0.1488800 secAQ 0.1488800 secRG 11585.2RG 11585.2RG 11585.2RG 11585.2DW 145.200 usecDW 145.200 usecDW 145.200 usecDW 145.200 usecDE 6.00 usecDE 6.00 usecDE 6.00 usecDE 6.00 usecTE 300.0 KTE 300.0 KTE 300.0 KTE 300.0 KD0 0.00000300 secD0 0.00000300 secD0 0.00000300 secD0 0.00000300 secD1 1.50000000 secD1 1.50000000 secD1 1.50000000 secD1 1.50000000 secD6 0.07000000 secD6 0.07000000 secD6 0.07000000 secD6 0.07000000 secD13 0.00000300 secD13 0.00000300 secD13 0.00000300 secD13 0.00000300 secD16 0.00050000 secD16 0.00050000 secD16 0.00050000 secD16 0.00050000 secIN0 0.00002380 secIN0 0.00002380 secIN0 0.00002380 secIN0 0.00002380 sec=CHANNEL f1=CHANNEL f1=CHANNEL f1=CHANNEL f1=NUC1 1HNUC1 1HNUC1 1HNUC1 1HP1 6.00 usecP1 6.00 usecP1 6.00 usecP1 6.00 usecP2 12.00 usecP2 12.00 usecP2 12.00 usecP2 12.00 usecPL1 -4.00 dBPL1 -4.00 dBPL1 -4.00 dBPL1 -4.00 dBSFO1 500.1326379 MHzSFO1 500.1326379 MHzSFO1 500.1326379 MHzSFO1 500.1326379 MHz=CHANNEL f2=CHANNEL f2=CHANNEL f2=CHANNEL f2=NUC2 13CNUC2 13CNUC2 13CNUC2 13CP3 15.70 usecP3 15.70 usecP3 15.70 usecP3 15.70 usecPL2 -2.00 dBPL2 -2.00 dBPL2 -2.00 dBPL2 -2.00 dBSFO2 125.7693867 MHzSFO2 125.7693867 MHzSFO2 125.7693867 MHzSFO2 125.7693867 MHz=GRADIENT CHANNEL=GRADIENT CHANNEL=GRADIENT CHANNEL=GRADIENT CHANNEL=GPNAM1 sine.100GPNAM1 sine.100GPNAM1 sine.100GPNAM1 sine.100GPNAM2 sine.100GPNAM2 sine.100GPNAM2 sine.100GPNAM2 sine.100GPNAM3 sine.100GPNAM3 sine.100GPNAM3 sine.100GPNAM3 sine.100GPX1 0.00%GPX1 0.00%GPX1 0.00%GPX1 0.00%GPX2 0.00%GPX2 0.00%GPX2 0.00%GPX2 0.00%GPX3 0.00%GPX3 0.00%GPX3 0.00%GPX3 0.00%GPY1 0.00%GPY1 0.00%GPY1 0.00%GPY1 0.00%GPY2 0.00%GPY2 0.00%GPY2 0.00%GPY2 0.00%GPY3 0.00%GPY3 0.00%GPY3 0.00%GPY3 0.00%GPZ1 50.00%GPZ1 50.00%GPZ1 50.00%GPZ1 50.00%GPZ2 30.00%GPZ2 30.00%GPZ2 30.00%GPZ2 30.00%GPZ3 40.10%GPZ3 40.10%GPZ3 40.10%GPZ3 40.10%P16 1000.00 usecP16 1000.00 usecP16 1000.00 usecP16 1000.00 usecF1-Acquisition parametersF1-Acquisition parametersF1-Acquisition parametersF1-Acquisition parametersND0 2ND0 2ND0 2ND0 2TD 256TD 256TD 256TD 256SFO1 125.7694 MHzSFO1 125.7694 MHzSFO1 125.7694 MHzSFO1 125.7694 MHzFIDRES 82.064079 HzFIDRES 82.064079 HzFIDRES 82.064079 HzFIDRES 82.064079 HzSW 167.039 ppmSW 167.039 ppmSW 167.039 ppmSW 167.039 ppmFnMODE undefinedFnMODE undefinedFnMODE undefinedFnMODE undefinedF2-Processing parametersF2-Processing parametersF2-Processing parametersF2-Processing parametersSI 1024SI 1024SI 1024SI 1024SF 500.1300144 MHzSF 500.1300144 MHzSF 500.1300144 MHzSF 500.1300144 MHzWDW QSINEWDW QSINEWDW QSINEWDW QSINESSB 0SSB 0SSB 0SSB 0LB 0.00 HzLB 0.00 HzLB 0.00 HzLB 0.00 HzGB 0GB 0GB 0GB 0PC 0.20PC 0.20PC 0.20PC 0.20F1-Processing parametersF1-Processing parametersF1-Processing parametersF1-Processing parametersSI 512SI 512SI 512SI 512MC2 QFMC2 QFMC2 QFMC2 QFSF 125.7577961 MHzSF 125.7577961 MHzSF 125.7577961 MHzSF 125.7577961 MHzWDW QSINEWDW QSINEWDW QSINEWDW QSINESSB 0SSB 0SSB 0SSB 0LB 0.00 HzLB 0.00 HzLB 0.00 HzLB 0.00 HzGB 0GB 0GB 0GB 0N N N NN N N NN N N NN N N NO O O OO O O OC C C CH H H H3 3 3 3C C C CH H H H3 3 3 3H H H H3 3 3 3C C C CH1 1 1 13 3 3 35 5 5 52 2 2 28 8 8 87 7 7 76 6 6 64 4 4 4Gradient HMBCGradient HMBCGradient HMBCGradient HMBCGradient HMBCGradient HMBCGradient HMBCGradient HMBC1 1 1 1 1 1 1 12 2 2 2 2 2 2 23 3 3 3 3 3 3 35 5 5 5 5 5 5 51 1 1 1 1 1 1 14 4 4 4 4 4 4 43 3 3 3 3 3 3 3 2 2 2 2 2 2 2 25 5 5 5 5 5 5 56 6 6 6 6 6 6 67 7 7 7 7 7 7 78 8 8 8 8 8 8 8CDClCDClCDClCDClCDClCDClCDClCDCl3 3 3 3 3 3 3 3CDClCDClCDClCDClCDClCDClCDClCDCl3 3 3 3 3 3 3 3http:/www.xy- 021-676323004.3.3 二维接力相关谱(2D Relayed NMR Spectroscopy)常规的常规的C,H-COSYC,H-COSYC,H-COSYC,H-COSY和和COLOCCOLOCCOLOCCOLOC谱以及反转实谱以及反转实验一般可以解决大部分有机分子的碳骨架验一般可以解决大部分有机分子的碳骨架连接问题，但有时候仍难以准确归属。二连接问题，但有时候仍难以准确归属。二维接力相关谱是可以提供碳碳骨架信息有维接力相关谱是可以提供碳碳骨架信息有用方法，它是用方法，它是C,H-COSYC,H-COSYC,H-COSYC,H-COSY谱的延伸谱的延伸 它有两种实验方法：氢接力的它有两种实验方法：氢接力的C,H-COSY C,H-COSY C,H-COSY C,H-COSY 谱和氢接力谱和氢接力的的H,H-COSYH,H-COSYH,H-COSYH,H-COSY谱谱 http:/www.xy- 021-67632300(1)氢接力的H,H-COSY谱（H-Relayed H,H-COSY）磁化矢量从磁化矢量从H H H HA A A A转移到转移到H H H HB B B B，再由，再由H H H HB B B B转移转移到到H H H HC C C C的接力，可检测出通过二根键、三根键和四的接力，可检测出通过二根键、三根键和四根键连接的质子间的交叉峰根键连接的质子间的交叉峰 CACBCCHAHBHchttp:/www.xy- 021-67632300谷氨酸 的氢接力H,H-COSY谱(p207)http:/www.xy- 021-67632300氢接力的C,H-COSY (H-Relayed C,H-COSY)磁化矢量从一个磁化矢量从一个1 1 1 1H H H H核（核（H H H HA A A A）磁化转移到邻位磁化转移到邻位的另一个的另一个1 1 1 1H H H H核（核（H H H HB B B B）上，再通过上，再通过H H H HB B B B把磁化把磁化转移到与其相连的转移到与其相连的C C C CB B B B的磁化矢量上，称之的磁化矢量上，称之H H H HA A A AH H H HB B B BC C C CB B B B接力。如果已确认接力。如果已确认了了H H H HA A A A，就可就可以通过以通过H H H HA A A A指认邻位指认邻位C C C CB B B B的化学位移的化学位移 HACACBHBhttp:/www.xy- 021-67632300CCCHaHbCHcHdhttp:/www.xy- 021-67632300http:/www.xy- 021-676323004.5 总相关谱(TOCSY谱)如果在氢接力如果在氢接力H,H-COSYH,H-COSY谱谱的脉冲序列中增加接力的级数，偶的脉冲序列中增加接力的级数，偶合相关的传递将逐渐增加。因此，从理论上讲，随着级数的合相关的传递将逐渐增加。因此，从理论上讲，随着级数的不断增加，将显示自旋体系的全部相关峰。但接力级数增不断增加，将显示自旋体系的全部相关峰。但接力级数增加，灵敏度下降很快，检测时间也会变得越来越长，限制了加，灵敏度下降很快，检测时间也会变得越来越长，限制了接力级数的增加。接力级数的增加。TOCSYTOCSYTOCSYTOCSY谱就是通过特殊的脉冲序列，实现从一个氢核的谱谱就是通过特殊的脉冲序列，实现从一