1、orionis biotek corporation 51101 / 2003-07- 2002(cad)pcr(taqman)pcr(cad)pcr(cad)pcrdnasars(cad)pcr(cad)pcr(cad)pcr30% 1.1. (cad)pcr(taqman)pcr1.2. sarssarssars1.3. 2.1. 2.2. 2.3. (appliedbiosystem)(cad)pcrchironmba()(abbott)(pharmacia)(pfizer)(celgen)(cad)pcr(cad)pcr3.1 3.1.1. (dnarna)(dna)3.1.2. 3.
2、1.2.1. 3.1.2.2. 3.1.2.3. 503.1.2.4. 3.1.2.5. 3.1.2.6. 3.1.3. (dna)3.1.4. ()(dna)dnapcr(dna)(cad)pcr() 3.2 pcrpcr(cad)pcrpcr3.2.1. pcrpcr()dnarnadnadnadna3.2.2. pcrdnadnaroche(taqman)pcr3.2.3. pcrdnadnadnapcrpcrdna(taqman)pcr3.3 (taqman)pcrrochetaqman(taqman)pcr3.3.1.3.3.1.1. 23.3.1.2. 3.3.1.4. 3.3.1
3、.5. dnapcr3.3.2. (taqman)pcr(taqman)pcrdna(taq)3.3.2.1. dnapcr(<100)sars3.3.2.2. (taqman)pcr(taqman)pcrdna3.3.2.3. dnadna(taqman)pcr(taqman)pcr,3.3.2.4. (taqman)pcrdna3.3.2.5. (taqman)pcrroche(taqman)pcrsarssarssarssars(taqman)pcrrocherochesarsroche(cad)pcrsars3.4 (cad)pcr3.4.1. (cad)pcr(cad)pcrp
4、crpcr(cad)pcr(taqman)pcr(taqman)pcr(cad)pcr2002 (taqman)pcr(cad)pcrdna(cad)pcr(taqman)pcrpcr3.4.2. pcrdnadnapcrdna(cad)pcrpfuultra(taqman)pcrtaq18(cad)pcr(cad)pcrdna(taqman)pcr303.4.3. (cad)pcr(taqman)pcr(cad)pcr(taqman)pcr(cad)pcr3.4.3.1. (taqman)pcr(cad)pcrabipcrprizm7000pcr(taqman)pcrpcrabipcrdna
5、1010000abipcrdna(taqman)pcrabipcr20pcrabi6200.20.dna(taqman)pcrpcr(taqman)pcr(cad)pcr(cad)pcrdna(taqman)pcr(taqman)pcr(taqman)pcr(cad)pcr(taqman)pcr(cad)pcr(taqman)pcr3.4.3.2. pcrpcr(taqman)pcr(cad)pcrsnp 10k 1 .1 .01 ct = -3.33lgc + 39.19 r2: 0.994 efficiency:99.7% ct = -3.66lgc + 40.31 r2: 0.995 efficiency:87.6% 10k 1 .1 .01(taqman)pcr(cad)pcrdna10000100010010dna3.4.3.3. pcrsdnadnadna(taqman)pcrdnadnadnadna