TM29基因在番茄花序及果实发育中作用.pptx

Down-RegulationofTM29,aTomatoSEPALLATAHomolog,CausesParthenocarpicFruitDevelopmentandFloralReversionCharles Ampomah-Dwamena,Bret A.Morris2,Paul Sutherland,Bruce Veit3,and Jia-Long YaoZhangYunyi09/06/16SEP functions in ArabidopsisSequenceanalysisofTM29Spatial and temporal expression of TM29in tomatoTM29transgenic plant phenotypesTM29 protein interacts with SlHDACsConclusionReferencesContextSEP functions in ArabidopsisBandCfloralorganidentityfunctionsrequireSEPALLATAMADS-boxgenesPelaz et al.,Nature405,200-203(11 May 2000)SEP functions in ArabidopsisSequenceanalysisofTM29Spatial and temporal expression of TM29in tomatoTM29transgenic plant phenotypesTM29 protein interacts with SlHDACsConclusionReferencesContextTM29Groups to the SEPSubfamilyPhylogeneticanalysisSequenceanalysisSEP functions in ArabidopsisSequenceanalysisofTM29Spatial and temporal expression of TM29in tomatoTM29transgenic plant phenotypesTM29 protein interacts with SlHDACsConclusionReferencesContextSpatial and Temporal Expression of TM29in Tomatofb,Flowerbudsfr,Youngfruitslf,Youngleavessh,Shoottipsrt,RootsNorthern-blothybridizationpa,Pre-anthesisa,AnthesisDPA,Fruitat3,6,14,and21dayspost-anthesislf,YoungleavesTM29 expressionoccursfromtheearlystagesofflowerdevelopmenttotheearlystagesoffruitdevelopment.Spatial and Temporal Expression of TM29in TomatoSympodialbudFloral(fm)/Inflorescence(im)meristemSepalOvaryInsituhybridizationsb,sympodialbud;lf,leaf;fm,floralmeristem;im,inflorescencemeristem;vb,vascularbundle;se,sepalprimordiuman,antherregion;ov,ovaryprimordium;per,pericarpregion;st,stamen;tap,tapetalregion;SEP functions in ArabidopsisSequenceanalysisofTM29Spatial and temporal expression of TM29in tomatoTM29transgenic plant phenotypesTM29 protein interacts with SlHDACsConclusionReferencesContextTM29Transgenic PlantsPhenotypesSepallata-likeflowersDelayedsenescencepetalstamenTM29Transgenic PlantsPhenotypesDelayedripeningprocessEctopicshootsgrewinsidethefuritTM29Transgenic PlantsPhenotypesel,Ectopicleaf;ef,Ectopicflower;fr,Fruit;es,Ectopicinflorescence;ov,ovule;per,Pericarp;pl,Placenta;sd,SeedTM29mayberequiredforthemaintenanceoffloralmeristemidentifySEP functions in ArabidopsisSequenceanalysisofTM29Spatial and temporal expression of TM29in tomatoTM29transgenic plant phenotypesTM29 protein interacts with SlHDACsConclusionReferencesContextvTM29 protein interacts with SlHDACsSlHDACs=SolanumlycopersicumHistone DeacetylasesYeasttwo-hybridassayPull-downassaySEP functions in ArabidopsisSequenceanalysisofTM29Spatial and temporal expression of TM29in tomatoTM29transgenic plant phenotypesTM29 protein interacts with SlHDACsConclusionReferencesContextTM29 shares a high amino acid sequence homology to the Arabidopsis SEP1,2,and 3 genesTM29 is involved in floral organs development like SEP genesTM29 may also play a role in the maintenance of floral meristem identity and fruit development in tomatoTM29 protein may recruit HDACs to regulate gene expression in reproductive development in tomato ConclusionSEP functions in ArabidopsisSequenceanalysisofTM29Spatial and temporal expression of TM29in tomatoTM29transgenic plant phenotypesTM29 protein interacts with SlHDACsConclusionReferencesContextSoraya Pelaz1,Gary S.Ditta1,Elvira Baumann2,Ellen Wisman2&Martin F.Yanofsky1(2000)B and C floral organ identity functions require SEPALLATA MADS-box genes.Nature 405,200-203Charles Ampomah-Dwamena,Bret A.Morris,2 Paul Sutherland,Bruce Veit,3 and Jia-Long Yao4,*(2002)Down-Regulation of TM29,a Tomato SEPALLATA Homolog,Causes Parthenocarpic Fruit Development and Floral Reversion.Plant Physiol.2002 Oct;130(2):605617Flanagan CA1,Ma H(1994)Spatially and temporally regulated expression of the MADS-box gene AGL2 in wild-type and mutant arabidopsis flowers.Plant Mol Biol.1994 Oct;26(2):581-95Linmao Zhao,JingxiaLu,JianxiaZhang,Pei-YingWu,SongguangYang and KeqiangWu(2014)Identification and characterization of histone deacetylases in tomato(Solanumlycopersicum).Front Plant Sci.2015 Jan 6;5:760References