2023年中国农业分子育种行业发展白皮书.pdf

亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)目录C O N T E N T S?1.1?锋?务?1.2?筛?1.3?1.4?务?务?12.1?2.2?2.3?2.4?2.5?2.6?BT?IT?2?4.1?务?4.2?务?4.3?务?4?5.1?筛?模?5.2?5.5?5.3?5.4?撑?5.6?5.7?5?33.1?愿?3.2?副?3.3?务?3.4?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)目录C O N T E N T S?1.1?锋?务?1.2?筛?1.3?1.4?务?务?12.1?2.2?2.3?2.4?2.5?2.6?BT?IT?2?4.1?务?4.2?务?4.3?务?4?5.1?筛?模?5.2?5.5?5.3?5.4?撑?5.6?5.7?5?33.1?愿?3.2?副?3.3?务?3.4?01/02亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?锋?务?锋?撰?锋?群?锋?务?撰?务?撰?研?研?2014?2022?务?51,851.1厅?84,438.6厅?8?撑?CARG?6.29%?务?务?2014-2022?务?6.06厅?6.87厅?8?撑?CARG?1.57%?95%?副?470?1 务?1.1?1.2?1.3?1.4 务?尊?务?51,851.1?54,205.3?55,659.9?58,059.8?61,452.6?66,066.5?71,748.2?78,339.5?84,438.6?201420152016201720182019202020212022?2014-2022?务?2014-2022?务?63,964.8?66,060.3?66,043.5?66,160.7?65,789.2?66,384.3?66,949.2?68,284.8?68,652.8?201420152016201720182019202020212022?研?务?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)5u?锋?务?务?告?务?副?撰?务?赠?筛?务?务?务?40%?28%?群?务?25.3%?锋?筛?锋?副?务?719.6?706.8?729.9?755.3?793.2?791.0?760.6?843.5?982.6?1,225.4?1,168.8?1,115.7?1,258.6?1,371.5?1,509.7?1,708.7?2,198.2?2,360.6?201420152016201720182019202020212022?2014-2022?务?务?副?务?副?副?2022?务?务?务?务?去?25.3%?研?务?盟?研?务?40%?28%?1 务?1.1?1.2?1.3?1.4 务?尊?务?研?务?03/04亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?锋?务?务?2014?2022?务?13,506?12,760?8?撑?-0.71%?务?锐?18厅占?务?撰?2019?2020-2022?务?筛?粒?2022?务?6,667?52.1%?务?研?络?研?务?96%?副?95%?75%?45%?40%?务?2017-2018?告?2017?5,599?/?5,849?/?4.47%?务?2014-2022?务?2014-2022?务?13,505.7?13,499.9?13,492.1?13,486.3?13,499.1?12,786.2?12,786.7?12,786.0?12,760.1?-0.1%0.0%-0.1%0.0%0.1%-5.3%0.0%0.0%-0.2%-1 00%-8 0%-6 0%-4 0%-2 0%0%20%-3 400160 0660 0116 00166 00216 00266 00201 4201 5201 6201 7201 8201 9202 0202 1202 2?(?)?5,445.9?5,553.0?5,539.2?5,607.4?5,621.2?5,719.7?5,733.5?5,805.0?5,801.7?0.1%2.0%-0.2%1.2%0.2%1.8%0.2%1.2%-0.1%-1 0.50%-8.50%-6.50%-4.50%-2.50%-0.50%1.50%3.50%530 0540 0550 0560 0570 0580 0590 0600 0610 0620 0630 0201 4201 5201 6201 7201 8201 9202 0202 1202 2?/?1 务?1.1?1.2?1.3?1.4 务?尊?务?研?务?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?锋?务?去?/?务?研?务?研?务?撑?40%?2021?3.5厅?锋?务?锋?务?务?2019?务?0.302?0.678?0.466?0.353?0.318?5?务?务?173435701211611661812523502012201320142015201620172018201920202021?2013-2021务?2019?10?1?1?0.4160.5601.0000.6782?1.0000.1040.1120.4663?0.5980.1710.1990.3534?0.2661.0000.0300.3185务?0.0080.2880.6030.3026?0.2120.0000.1450.1437?0.1240.0000.1040.0918?0.0560.0790.0510.0599?0.1140.0000.0010.04510资?千0.0660.0000.0000.0261 务?1.1?1.2?1.3?1.4 务?尊?务?705/06?研?1?务?务?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?锋?务?务?务?尽?2022?务?副?TOP10?尽?盟?研?/?务?2.9%?2022?11.4%?务?务?务?盛?撰?务?务?锋?2020?锋?CR51?52%?务?CR5?12%?2020?锋?CR32?47%?CR5?5%?研导?务?锋?副?务?2022?务?2020?23.0%14.0%10.0%53.0%PIC?DanBred?Topigs?Norsvin?2020?5.0%95.0%CR5?2020?务?2020?务?20.0%17.0%7.0%4.0%4.0%48.0%?Vilmorin?4.0%3.0%2.0%2.0%1.0%88.0%?2.9%11.4%务?1 务?1.1?1.2?1.3?1.4 务?尊?务?研?1?CR5?2?CR3?Kynetec?务?务?PIC?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)?研?u 筛?筛?筛?2021?务?2023?务?筛?锐?/?1 务?1.1?务?1.2?1.3?1.4 务?尊?务?907/08亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u 筛?筛?撰?筛?赠?筛?务?务?去?去?20212021?1 1?务?去?去?20212021?7 7?务?去?务?20212021?8 8?2021-2035?务?2025?2030?2035?20212021?1010?2021-2023?20212021?3 3?2035?1 务?1.1?务?1.2?1.3?1.4 务?尊?务?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u 筛?20232023?1 1?2023?务?的?2020?12?-2023?8?20222022?1 1?2022?务?去?20222022?5 5?务?20222022?8 8?务?20222022?3 3?去?去?去?20232023?4 4?2023?20232023?2 2?务?务?2023?1 务?1.1?务?1.2?1.3?1.4 务?尊?务?1109/10?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u 筛?2021?2023?务?筛?2021?务?2022?2022?2023?务?的?锐?2023?务?2023?络?盟?务?络?络?副?务?务?去?去?去?。?去?去?的?的?20212021?20222022?20232023?1 务?1.1?务?1.2?1.3?1.4 务?尊?务?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u 筛?2021?3?去?务?够?2035?务?络?务?撑?2023?2?务?务?2023?络?络?务?撑?络?去?络?络?络?撰?1?络?2?络?络?络?3?锐?络?络?4?模?赠?络?锐?络?撰?络?络?络?络?去?络?络?锐?去?1 务?1.1?务?1.2?1.3?1.4 务?尊?务?1311/12?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u 筛?副?筛?2023?络?2023?筛?研?锐?锐?筛?映?模?筛?务?副?映?2023?202120212022202220232023?2021-2023?务?映?模?筛?1 务?1.1?务?1.2?1.3?1.4 务?尊?务?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?2023?6?16?务?务?筛?络?络?务?络?筛?映?尽?尽?尽?CRO?告?BT+IT?副?尽?尽?务?盟?务?1121150135115012143201820192020202120222023.1-8?1 务?1.1?务?1.2?1.3?1.4 务?尊?务?13/14?研?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?副?的?副?撰?务?络?撰?溶?尽?务?务?务?务?尽?的?务?2022?3.06厅?副?锐?够?锐?筛?副?锐?务?2022?2023?1?-8?去?务?研?筛?告?务?2023-09-264?务?/?B+?2023-08-27?3?/?/?/?/?/?/?2023-08-20?务?2023-08-18?2023-08-16?/?/精?/?务?Pre-A?2023-08-10?2023-06-19?1 务?1.1?务?1.2?1.3?1.4 务?尊?务?研?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?亿欧智库：近三年中国境内重点种业厂商融资事件列表?2023-05-214306.67?/?/务?(?)?Pre-A?2023-04-25?2023-04-242.24?2023-04-201005.96?2023-04-191006?Pre-A?2023-04-19?/?/?/务?/?务?Pre-A?2023-03-224000?2023-03-071100.0?2023-03-06?/务?2023-02-263117.08?2023-02-051500.0?A+?2023-02-20?/精?/?/?/?/?/?A?2022-12-31?务?2022-12-142323.52?2022-10-31?IDG?2022-10-25?油?/?/?2022-10-11?务?2022-09-2923.33?/?/?/?C?2022-08-30?/?2022-07-05?2022-04-243.06?2022-04-28?2022-04-242.6?B?2022-04-211?/?/?/?/?研?Pre-A?2022-03-23?/?2022-03-201?2022-03-132700?务?2022-02-07700?IDG?/?/?/务?2022-01-261.49?2021-12-21835.44?2021-10-171513.2?务?1 务?1.1?务?1.2?1.3?1.4 务?尊?务?15/16?研?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u 务?务?锐?映?1.0?2.0?3.0?4.0?1 1.0 0?务?去?2 2.0 0?20?务?3 3.0 0?20?4 4.0 0?+?+?研?尽?3.0?4.0?务?2.0?3.0?务?务?务?1.01.0?去?去?2.02.0?去?去?3.03.0?去?去?撰?4.04.0?去?去务?1 务?1.1?务?1.2?1.3?1.4?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u 务?务?务?务?导?务?筛?务?务?务?务?务?19491949-19781978?19791979-19991999?20002000-20102010?务?务?筛?筛?筛?筛?模?务?务?务?模?务?20112011-20212021?去?20212021?筛?筛?送?务?务?1 务?1.1?务?1.2?1.3?1.4?17/18?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u 务?务?撰?务?2.0?3.0?务?务?52?告?模?务?告?模?副?筛?务?务?副?研?研?副?副?模?务?1 务?1.1?务?1.2?1.3?1.4?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)目录C O N T E N T S19/20?1.1?锋?务?1.2?筛?1.3?1.4?务?务?12.1 分子育种技术的发展历程与现状综述2.2 分子标记辅助育种：利用分子标记选择目标性状2.3 转基因技术：引入外源基因产生可预期的、定向的遗传改变2.4 基因编辑技术：精确修改目标基因位点2.5 合成生物学：人工设计调控元件和调控线路，来改变生物性状2.6 BT与IT交叉融合的智慧育种：精准化、高效化、智能化种业技术革命2?4.1 分子育种技术在畜禽水产作物中产业应用情况4.2 中国分子育种产业化进程的国际差距分析4.3 分子育种产业化进程中存在的问题与制约因素分析4?5.1 分子育种政策持续完善，推动行业快速发展5.2 强化种质资源库建设和产业应用5.5 进一步深化分子育种产学研跨领域沟通5.3 企业引领分子育种行业发展5.4 智能分子育种需要复合型人才持续储备5.6 知识产权保护是分子育种行业发展的“护城河”5.7 分子育种与农业数字化有机结合，促进农业发展智慧化变革5?33.1 分子育种产业链概述与产业链图谱3.2 产业链上游结构属性和价值分析3.3 产业链中游结构属性和价值分析3.4 产业链下游结构属性和价值分析亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?MAS?Marker-assistedselection?GS?Genomic selection?溶?溶?务?务?研?副?的?去?撰?粉?去?溶模?愿?副?群?锐?锐?研?去?l?l?l?l?l 务?996?务?998?l?l?l DNA?l?粒?撑?l ZFNs?l TALENs?l CRISPR/Cas?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?19?务?20?1953?DNA?20?70?90?的?RAPD?的?AFLP?SSR?SNP?1去?2去?3去20?70?20?80?20?90?1973?1982?1983?1986?1994?副?副?1996?21?SangerSanger?2013?2015?21?10?1996?ZFN?锐?映?副?盐?2011?TALEN?CRISPR/Cas9?RFLP?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?21/22?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?DNA?20?90?去?映?务?DNA?务?副?覆?务?-?-?模?模?务?去?Stereo-seq?愿?1?相较传统的Bulk测序，单细胞测序技术可以更好的区分异质性细胞，捕捉到不同细胞类型的基因表达特征，绘制细胞图谱，筛选出调控关键性状的候选基因，例如胁迫响应或发育相关的基因等，支持鉴定和挖掘关键基因功能，为动植物的性状改良和育种提供新的技术方法。?Stereo-seq?务?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?研?1 Hao,?F.,?Liu,?X.,?Zhou,?B.?et?al.?Chromosome-level?genomes?of?three?key?Allium?crops?and?their?trait?evolution.?Nat?Genet?55,?1976?1986?(2023).?https:/doi.org/10.1038/s41588-023-01546-0?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?锋?Gb?Tb?务?愿?愿?愿?研?研?研?研?研?务?研?撑?MGISP-384?MGISP-100?MGISP-960?撑?NGS?DNBSEQ-T7?MGISEQ-2000?DNBSEQ-G99?MGISEQ-200?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?23/24?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?务?愿?溶?模?溶?去?研?告?溶?研?研?研?研?研?去?研?映?研?务?溶?告?务?研?副?研?研?撰?研?务?1?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?研?1?,?,?.?研?J.务?,2023,25(04):227-238.?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?MAS?MAS?去?务?-?453?RAD-Seq(?BGISEQ-500?Geneseek?Procine?50K?SNP?SNP?RAD-Seq?撰?SNP?告?RAD-seq?SNP?SNP?3?1?务?务?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?25/26?研?1?.?SNP?务?D.?务?,?2020.?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)42,527?35,535?31,792?17,532?8,752?8,656?9,026?5,335?4,409?1,348?732?20122013201420152016201720182019202020212022u?务?告?撑?QTL?撑?撰?撰?Genomic Selection?GS?副?撑?务?尽?DNBSEQ-T7?DNBSEQTM?Adapter Rate?Duplication Rate?撑?IndexHopping?溶?务?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?去?务?溶?锋?锐?锐?Ti?Ri?T-DNA?群?T-DNA?务?DNA?务?副?DNA?务?务?务?务?务?DNA?LTR?副?群?务?务?2.分子育种技术是种业革?2.1?2.2?2.3?2.4?2.5?尊?2.6 BT?IT?27/28?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?锋?撑?/?/?/?撰?锋?副?锋?98%?副?粒?A?/?导?III?ATryn?溶?副?副?副?导?rhSA?溶?务?48.9%32.7%12.6%4.9%0.9%?2022?169.20%28.30%2.50%?2022?III(ATryn)III(ATryn)?副?导?Note:?HT?indicates?herbicide-tolerant?varieties;?Bt?(Bacillus?thuringiensis)?indicates?insect-resistant?varieties?(containing?genes?from?the?soil?bacterium?Bt).?Data?for?HT/Bt?cornand?cotton?are?not?mututally?exclusive,?as?HT?and?Bt?categories?include?those?varieties?with?overlapping?(stacked)?HT?and?Bt?traits.Source:?USDA,?Economic?Research?Service?using?data?from?the?2002?ERS?report,?Adoption?of?Bioengineered?Crops?(AER-810)?for?1996-99?and?National?Agricultural?Statistics?Service(annual)?June?Agricultural?Survey?for?2000-23?1996-2023?2.分子育种技术是种业革?2.1?2.2?2.3?2.4?2.5?尊?2.6 BT?IT?研?1?http:/ 38570-2020?溶?PCR?PCR?PCR?群?群?导?GB/T?38570-2020?务?务?尽?络?务?务?尽2.分子育种技术是种业革?2.1?2.2?2.3?2.4?2.5?尊?2.6 BT?IT?29/30?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?DNA?ZFNs?TALENs?CRISPR?ZFNs?TALENs?溶?撰?务?告?DNA?CRISPR/Cas?DNADNA?DNDNA A?ZFNs?ZFNs?DNA?C?Fok I?ZF?Fok I?3-4?ZF?9-12bp?*23?TALENsTALEN?TALE?DNA?Fok?TALE?TALE?RVD?Fok I?8-31?8-31bp?*21?ZFN?0-34%CRISPR/Cas9?CRISPR/Cas9?Cas9?研?sgRNA?Cas9?gRNA?DNA?DNA?RNACas9?RNA?20bp+NGG*11?PAM?割?NHEJ?5151-7979%2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?2012?CRISPR-Cas9?去?务?去CRISPR?溶?撰?告?去?溶?DNA?锋?溶?去?去?DNA霞?群?撰?溶?CRISPR-Cas?去?去?务?DNA?DNA?撑?/?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?31/32?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?溶?溶?粒?1?溶?35?2?务?溶?粒?4567?务?务?锋?副?撰?2,3?2016?2021?务?溶?32.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?研?1?.?,?M.?:务?,20202?,?,?,?.?务?J.?,2020,28(12):2230-2239.3ZHU?H,?LI?C,?GAO?C.?Applications?of?CRISPR-Cas?in?agriculture?and?plant?biotechnologyJ.?Nat.?Rev.?Mol.?Cell?Biol.,2020,21(11):661-677.4?Lu,?Y.,?Wang,?J.,?Chen,?B.,?Mo,?S.,?Lian,?L.,?Luo,?Y.,?.?&?Jiang,?L.?(2021).?A?donor-DNA-free?CRISPR/Cas-based?approach?to?gene?knock-up?in?rice.?Nature?plants,?7(11),?1445-1452.5?,?,?.?J.?,2017,35(02):1-22.DOI:10.19588/?j.issn.1003-935X.2017.02.001.6?.?D.?务?,2006.7?.?D.?务?,2019.?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?CRISPR?务溶?告?副?去?务?研?研?NatureBiotechnology?DNBSEQ-T7?模?Digenome-seq?CRISPR-Cas12f1?2?副?DNBSEQ-T7?研?Sanger?NGS?IDLVs?BLESS?GUIDE-seq?告?DSB?DSB 太?Digenome-seq?Circle-seq?SITE-seq?Cas9?1?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?33/34?研?1?,?,?,?,?.CRISPR/Cas9?务?J.?,2020,36(03):78-87.DOI:10.13560/ki.biotech.bull.1985.2019-0871.2Kim,D.,Bae,S.,Park,J.,Kim,E.,Kim,S.,Yu,H.R.,.&Kim,J.S.(2015).Digenome-seq:genome-wideprofiling of CRISPR-Cas9 off-targeteffects in human cells.Naturemethods,12(3),237-243.?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u?撰?1?2?务?DNA?撰?务?务?副?务?务?务?去?锋?去?尽Ginkgo Bioworks?尽Joyn Bio?务?CRISPR?尽?Pairwise?Tropic?Biosciences?Joyn Bio?Pivot?Bio?Zymergen?Provivi?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?2.6 BT?IT?研?1?,?,?.?J .?,2020,1(02):121-140.2?.务?J .务?:?,2019,49(12):1543-1572.ZHANG X E.Synthetic biology in China:reviewand prospects J.Sci.Sin.(Vitae),2019,49(12):1543-1572.?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)u BT?IT?撰?撑?撰?Biological Technology?BT?Information Technology?IT?4.0?BT?IT?去?3.0?BT?4.0?覆?BT?研?覆?IT?研?研?研?撰?撰?研?筛?筛?务?筛?络?络?够?够?务?2025?副?够务?确?务?2020?12?4?去?务?BT+IT?副?2022副?送?BT+IT?副?副?BT+IT?务?研?盟?研?AI?BT+IT?送?BT+IT?务?2.分子育种技术是种业革?2.1?2.2?2.3?尊2.4?2.5?尊?2.6 BT?IT?35/36?研?亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿欧智库-李叶(396226)亿